Design and Testing of β-Actin Primers for RT-PCR that Do Not Co-amplify Processed Pseudogenes
Quantitative reverse transcription polymerase chain reaction (RT-PCR) is being used increasingly as an alternative to Northern blots analysis or RNase protection assays for quantitation of gene expression. To quantify different samples, measurements are often normalized using the expression of so-ca...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97233st02 |
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