Protocol to perform polysome profiling in primary differentiating murine adipocytes

Protocol to perform polysome profiling in primary differentiating murine adipocytes

Summary: Here, we present a protocol for polysome profiling in differentiating adipocytes from the mouse stromal vascular fraction. We describe steps for lysate preparation, ultracentrifugation through sucrose gradients, mRNA isolation, RNA sequencing, and motif enrichment. This protocol enables the...

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Bibliographic Details
Main Authors: Mirian Krystel De Siqueira, Zaynab Nouhi, Yutian Zhao, Siqi Wang, Xinshu Xiao, Xia Yang, Laura Hulea, Claudio J. Villanueva
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Cell Biology
RNA-seq
Metabolism
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002059
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