Screening and stress tolerance of yeasts in Morella nana fermentation broth
Using Morella nana (A. Chev.) J. Herb as raw material to process natural fermentation, and yeast strains were isolated and purified by extracting yeast extract peptone dextrose (YPD) medium and wort medium from the fermentation broth. The screened strains were tested for ester production, ethanol pr...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-03-01
|
| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-145.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850077151684984832 |
|---|---|
| author | JIANG Yurou, CAI Jian, GAO Xiu, XU Qingfang, LIU Weiliang, XU Qihe, ZHANG Lifang, WANG Yuchen, ZHU Ling |
| author_facet | JIANG Yurou, CAI Jian, GAO Xiu, XU Qingfang, LIU Weiliang, XU Qihe, ZHANG Lifang, WANG Yuchen, ZHU Ling |
| author_sort | JIANG Yurou, CAI Jian, GAO Xiu, XU Qingfang, LIU Weiliang, XU Qihe, ZHANG Lifang, WANG Yuchen, ZHU Ling |
| collection | DOAJ |
| description | Using Morella nana (A. Chev.) J. Herb as raw material to process natural fermentation, and yeast strains were isolated and purified by extracting yeast extract peptone dextrose (YPD) medium and wort medium from the fermentation broth. The screened strains were tested for ester production, ethanol production and H<sub>2</sub>S production, screened by Duchenne tubular fermentation experiments, and then the tolerance tests of the yeasts were conducted. Finally, the screened yeasts were identified by molecular biological identification based on 26S rDNA gene fragment sequence. The results showed that 59 yeasts were isolated and purified from the fermentation broth of M. nana, and 3 superior yeasts were screened and numbered JYR-5, JYR-14 and JYR-17. These three yeast strains could still grow normally under the condition of temperature 40 ℃, ethanol volume fraction 5%, pH 2.5, glucose concentration 300 mg/L, and SO<sub>2</sub> concentration 400 mg/L. Strains JYR-5 and JYR-14 were identified as Saccharomyces cerevisiae, and strain JYR-17 was identified as Pichia kudriavzevii. Therefore, three yeasts screened from the fermentation broth of M. nana provided certain reference value for the development and utilization of microbial resources for bayberry wine brewing. |
| format | Article |
| id | doaj-art-1111ba08ef114c3fbd5810d62af9dbb4 |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-1111ba08ef114c3fbd5810d62af9dbb42025-08-20T02:45:52ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-03-0143314515010.11882/j.issn.0254-5071.2024.03.023Screening and stress tolerance of yeasts in Morella nana fermentation brothJIANG Yurou, CAI Jian, GAO Xiu, XU Qingfang, LIU Weiliang, XU Qihe, ZHANG Lifang, WANG Yuchen, ZHU Ling01. Yunnan Engineering Research Center of Fruit Wine, Qujing Normal University, Qujing 655011, China;;2. School of Chemistry, Biology and Environment, Yuxi Normal University, Yuxi 653100, ChinaUsing Morella nana (A. Chev.) J. Herb as raw material to process natural fermentation, and yeast strains were isolated and purified by extracting yeast extract peptone dextrose (YPD) medium and wort medium from the fermentation broth. The screened strains were tested for ester production, ethanol production and H<sub>2</sub>S production, screened by Duchenne tubular fermentation experiments, and then the tolerance tests of the yeasts were conducted. Finally, the screened yeasts were identified by molecular biological identification based on 26S rDNA gene fragment sequence. The results showed that 59 yeasts were isolated and purified from the fermentation broth of M. nana, and 3 superior yeasts were screened and numbered JYR-5, JYR-14 and JYR-17. These three yeast strains could still grow normally under the condition of temperature 40 ℃, ethanol volume fraction 5%, pH 2.5, glucose concentration 300 mg/L, and SO<sub>2</sub> concentration 400 mg/L. Strains JYR-5 and JYR-14 were identified as Saccharomyces cerevisiae, and strain JYR-17 was identified as Pichia kudriavzevii. Therefore, three yeasts screened from the fermentation broth of M. nana provided certain reference value for the development and utilization of microbial resources for bayberry wine brewing.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-145.pdfmorella nana| saccharomyces cerevisiae|stress tolerance|screening |
| spellingShingle | JIANG Yurou, CAI Jian, GAO Xiu, XU Qingfang, LIU Weiliang, XU Qihe, ZHANG Lifang, WANG Yuchen, ZHU Ling Screening and stress tolerance of yeasts in Morella nana fermentation broth Zhongguo niangzao morella nana| saccharomyces cerevisiae|stress tolerance|screening |
| title | Screening and stress tolerance of yeasts in Morella nana fermentation broth |
| title_full | Screening and stress tolerance of yeasts in Morella nana fermentation broth |
| title_fullStr | Screening and stress tolerance of yeasts in Morella nana fermentation broth |
| title_full_unstemmed | Screening and stress tolerance of yeasts in Morella nana fermentation broth |
| title_short | Screening and stress tolerance of yeasts in Morella nana fermentation broth |
| title_sort | screening and stress tolerance of yeasts in morella nana fermentation broth |
| topic | morella nana| saccharomyces cerevisiae|stress tolerance|screening |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-145.pdf |
| work_keys_str_mv | AT jiangyuroucaijiangaoxiuxuqingfangliuweiliangxuqihezhanglifangwangyuchenzhuling screeningandstresstoleranceofyeastsinmorellananafermentationbroth |