HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male mice

Abstract Viral infections are associated with the disruption of oxidative stress and the progression of inflammatory mechanisms that play pivotal roles in cardiovascular diseases. In the present study, several inflammatory and oxidative stress markers were examined in HTLV‐1‐infected male BALB/c mic...

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Main Authors: Saeed Niazmand, S. A. Rahim Rezaee, Jamshid Gholizadeh Navashenaq, Nema Mohamadian Roshan, Mohsen Ghoryani, Houshang Rafatpanah, Maryam Mahmoudabady, Yousef Baghcheghi, Maryam Paseban, Mahdiyeh Hedayati‐Moghadam
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Physiological Reports
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Online Access:https://doi.org/10.14814/phy2.70409
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author Saeed Niazmand
S. A. Rahim Rezaee
Jamshid Gholizadeh Navashenaq
Nema Mohamadian Roshan
Mohsen Ghoryani
Houshang Rafatpanah
Maryam Mahmoudabady
Yousef Baghcheghi
Maryam Paseban
Mahdiyeh Hedayati‐Moghadam
author_facet Saeed Niazmand
S. A. Rahim Rezaee
Jamshid Gholizadeh Navashenaq
Nema Mohamadian Roshan
Mohsen Ghoryani
Houshang Rafatpanah
Maryam Mahmoudabady
Yousef Baghcheghi
Maryam Paseban
Mahdiyeh Hedayati‐Moghadam
author_sort Saeed Niazmand
collection DOAJ
description Abstract Viral infections are associated with the disruption of oxidative stress and the progression of inflammatory mechanisms that play pivotal roles in cardiovascular diseases. In the present study, several inflammatory and oxidative stress markers were examined in HTLV‐1‐infected male BALB/c mice. Twenty BALB/c mice were divided into two groups: the HTLV‐1‐infected group and the control group. Two months later, samples were collected from blood, aorta, heart, spleen, and lymph nodes. Finally, the levels of various plasma markers (lipid profile, creatine phosphokinase, nitric oxide, GSH, and total thiol), oxidative stress markers (SOD and CAT activity, MDA and total thiol levels), chemokine receptors genes expression (CCR2, CXCR2, CCR1) and eNOS expression in aortic and heart tissues, as well as histopathological changes in the heart, were evaluated. Plasma triglyceride, creatine phosphokinase, nitric oxide, and aorta malondialdehyde levels in the HTLV‐1‐infected group were higher than those in the control group. In contrast, total thiol levels in plasma, heart, and aorta, plasma glutathione levels, and the activities of superoxide dismutase and catalase were lower compared to the control group. The expression of CCR2 and CXCR2 was elevated in the aorta of the HTLV‐1‐infected group, while eNOS expression was reduced in both aortic and heart tissues. HTLV‐1 may contribute to inflammatory responses and oxidative stress in cardiovascular tissues.
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spelling doaj-art-1110fa7433904f9baebc620ad7935a232025-08-20T03:45:10ZengWileyPhysiological Reports2051-817X2025-06-011311n/an/a10.14814/phy2.70409HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male miceSaeed Niazmand0S. A. Rahim Rezaee1Jamshid Gholizadeh Navashenaq2Nema Mohamadian Roshan3Mohsen Ghoryani4Houshang Rafatpanah5Maryam Mahmoudabady6Yousef Baghcheghi7Maryam Paseban8Mahdiyeh Hedayati‐Moghadam9Department of Physiology, Faculty of Medicine Mashhad University of Medical Sciences Mashhad IranImmunology Research Center, Inflammation and Inflammatory Diseases Division Mashhad University of Medical Sciences Mashhad IranNoncommunicable Diseases Research Center Bam University of Medical Sciences Bam IranDepartment of Pathology, Ghaem Hospital Mashhad University of Medical Sciences Mashhad IranDepartment of Laboratory Sciences, School of Paramedical Sciences Torbat Heydariyeh University of Medical Sciences Torbat Heydariyeh IranImmunology Research Center, Inflammation and Inflammatory Diseases Division Mashhad University of Medical Sciences Mashhad IranDepartment of Physiology, Faculty of Medicine Mashhad University of Medical Sciences Mashhad IranBio Environmental Health Hazards Research Center Jiroft University of Medical Sciences Jiroft IranInnovative Medical Research Center and Department of Physiology, Faculty of Medicine Mashhad Medical Science Islamic Azad University Mashhad IranStudent Research Committee Jiroft University of Medical Sciences Jiroft IranAbstract Viral infections are associated with the disruption of oxidative stress and the progression of inflammatory mechanisms that play pivotal roles in cardiovascular diseases. In the present study, several inflammatory and oxidative stress markers were examined in HTLV‐1‐infected male BALB/c mice. Twenty BALB/c mice were divided into two groups: the HTLV‐1‐infected group and the control group. Two months later, samples were collected from blood, aorta, heart, spleen, and lymph nodes. Finally, the levels of various plasma markers (lipid profile, creatine phosphokinase, nitric oxide, GSH, and total thiol), oxidative stress markers (SOD and CAT activity, MDA and total thiol levels), chemokine receptors genes expression (CCR2, CXCR2, CCR1) and eNOS expression in aortic and heart tissues, as well as histopathological changes in the heart, were evaluated. Plasma triglyceride, creatine phosphokinase, nitric oxide, and aorta malondialdehyde levels in the HTLV‐1‐infected group were higher than those in the control group. In contrast, total thiol levels in plasma, heart, and aorta, plasma glutathione levels, and the activities of superoxide dismutase and catalase were lower compared to the control group. The expression of CCR2 and CXCR2 was elevated in the aorta of the HTLV‐1‐infected group, while eNOS expression was reduced in both aortic and heart tissues. HTLV‐1 may contribute to inflammatory responses and oxidative stress in cardiovascular tissues.https://doi.org/10.14814/phy2.70409aortaheartHTLV‐1inflammationoxidative stress
spellingShingle Saeed Niazmand
S. A. Rahim Rezaee
Jamshid Gholizadeh Navashenaq
Nema Mohamadian Roshan
Mohsen Ghoryani
Houshang Rafatpanah
Maryam Mahmoudabady
Yousef Baghcheghi
Maryam Paseban
Mahdiyeh Hedayati‐Moghadam
HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male mice
Physiological Reports
aorta
heart
HTLV‐1
inflammation
oxidative stress
title HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male mice
title_full HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male mice
title_fullStr HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male mice
title_full_unstemmed HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male mice
title_short HTLV‐1 infection altered expression of CCR2, CXCR2, eNOS genes, and oxidative stress in aorta and heart of male mice
title_sort htlv 1 infection altered expression of ccr2 cxcr2 enos genes and oxidative stress in aorta and heart of male mice
topic aorta
heart
HTLV‐1
inflammation
oxidative stress
url https://doi.org/10.14814/phy2.70409
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