Research progress on genes and enzymatic characteristics of proteases from Aspergillus oryzae

Aspergillus oryzae is a key microorganism in soy sauce brewing. The protease secreted by A. oryzae is mainly involved in the transformation process of brewing material proteins to amino acids and flavor peptides, which provides nutrients for the growth and fermentation of yeasts, lactic acid bacteri...

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Bibliographic Details
Main Author: WU Ribang, CHEN Chen, FANG Bowen, ZHOU Qiyang
Format: Article
Language:English
Published: Editorial Department of China Brewing 2024-04-01
Series:Zhongguo niangzao
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Online Access:https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-4-8.pdf
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Summary:Aspergillus oryzae is a key microorganism in soy sauce brewing. The protease secreted by A. oryzae is mainly involved in the transformation process of brewing material proteins to amino acids and flavor peptides, which provides nutrients for the growth and fermentation of yeasts, lactic acid bacteria and other microorganisms, and material basis for the formation of other flavor substances. Therefore, protease is an important index in the breeding of soy sauce fermentation strains. With the development of whole genome sequencing of A. oryzae, analyzing proteases of A. oryzae at the gene level has become an important pathway for discovering of new protease resources. At the same time, analyzing the cooperation among proteases and the relationship between proteases and flavor formation according to the enzymatic characteristics will provide guidance to the improvement of A. oryzae strains for soy sauce brewing. In this review, the gene mining current situation of A. oryzae proteases resources were analyzed from genome data of A. oryzae, and the enzymatic characteristics of reported proteases (secretory endopeptidase, secretory exopeptidase) from A. oryzae were reviewed, which provided references for new protease resource mining from A. oryzae and the development of new strains.
ISSN:0254-5071