Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i>
<i>Helicobacter pylori</i>, a spiral-shaped bacterium found in the stomach, is associated with various gastrointestinal and systemic health conditions. Effective suppression of <i>H. pylori</i> is therefore critical for managing gastrointestinal diseases. In a search for natu...
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MDPI AG
2025-07-01
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| author | So Yeong Jeong Dong-Min Kang Hyun-Jun Kim Sang Won Yeon Hak Hyun Lee Min Hee Kim Bang Yeon Hwang Mi-Jeong Ahn Mi Kyeong Lee |
| author_facet | So Yeong Jeong Dong-Min Kang Hyun-Jun Kim Sang Won Yeon Hak Hyun Lee Min Hee Kim Bang Yeon Hwang Mi-Jeong Ahn Mi Kyeong Lee |
| author_sort | So Yeong Jeong |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i>, a spiral-shaped bacterium found in the stomach, is associated with various gastrointestinal and systemic health conditions. Effective suppression of <i>H. pylori</i> is therefore critical for managing gastrointestinal diseases. In a search for natural products with anti-<i>H. pylori</i> activity, the extract of <i>Atractylodes macrocephala</i> rhizoma showed significant inhibitory effects. Chromatographic purification of <i>A. macrocephala</i> extract yielded thirteen compounds, which were identified as ten sesquiterpenes and three polyacetylenes by spectroscopic analysis. The sesquiterpene compounds belong to the eudesmane or eudesmane lactone types and exhibited structure-dependent efficacy. The major eudesmane lactone sesquiterpene, atractylenolide I (<b>1</b>), showed strong inhibitory activity comparable to metronidazole, a positive control, and atractylenolide III (<b>3</b>) also showed good efficacy. However, structural modification such as hydroxylation, methylation, or acetylation of the sesquiterpenes led to reduced activity. In contrast, polyacetylene derivatives displayed only mild inhibitory effects. Further evaluation of the active compounds against three <i>H. pylori</i> strains such as 51, 43504, and 26695 showed that atractylenolide I (<b>1</b>) had potent inhibitory effects against all three strains, with MIC<sub>50</sub> values of ranging from 27.3 to 48.6 μM and MIC<sub>90</sub> values from 45.4 to 87.2 μM. Atractylenolide III (<b>3</b>) exhibited selective activity against strain 51 with MIC<sub>50</sub> value of 89.9 μM. Both compounds also exhibited anti-inflammatory activity with IC<sub>90</sub> values of 23.3 and 31.1 μM, respectively, although they showed little effect on urease. This is the first report on the anti-<i>H. pylori</i> efficacy of various constituents of <i>A. macrocephala</i> and comparative analysis of inhibitory effects against several strains, which will provide scientific evidence supporting its potential as therapeutic agent for <i>H. pylori</i>-related infection. |
| format | Article |
| id | doaj-art-10f75b3dcd6c413d89cdab4f72fea4ae |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-10f75b3dcd6c413d89cdab4f72fea4ae2025-08-20T03:02:55ZengMDPI AGMolecules1420-30492025-07-013015314210.3390/molecules30153142Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i>So Yeong Jeong0Dong-Min Kang1Hyun-Jun Kim2Sang Won Yeon3Hak Hyun Lee4Min Hee Kim5Bang Yeon Hwang6Mi-Jeong Ahn7Mi Kyeong Lee8College of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of KoreaForest Medicinal Resources Research Center, National Institute of Forest Science, Yeongju 36040, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of KoreaCollege of Pharmacy and Research Institute of Pharmaceutical Sciences, Gyeongsang National University, Jinju 52828, Republic of KoreaCollege of Pharmacy, Chungbuk National University, Cheongju 28160, Republic of Korea<i>Helicobacter pylori</i>, a spiral-shaped bacterium found in the stomach, is associated with various gastrointestinal and systemic health conditions. Effective suppression of <i>H. pylori</i> is therefore critical for managing gastrointestinal diseases. In a search for natural products with anti-<i>H. pylori</i> activity, the extract of <i>Atractylodes macrocephala</i> rhizoma showed significant inhibitory effects. Chromatographic purification of <i>A. macrocephala</i> extract yielded thirteen compounds, which were identified as ten sesquiterpenes and three polyacetylenes by spectroscopic analysis. The sesquiterpene compounds belong to the eudesmane or eudesmane lactone types and exhibited structure-dependent efficacy. The major eudesmane lactone sesquiterpene, atractylenolide I (<b>1</b>), showed strong inhibitory activity comparable to metronidazole, a positive control, and atractylenolide III (<b>3</b>) also showed good efficacy. However, structural modification such as hydroxylation, methylation, or acetylation of the sesquiterpenes led to reduced activity. In contrast, polyacetylene derivatives displayed only mild inhibitory effects. Further evaluation of the active compounds against three <i>H. pylori</i> strains such as 51, 43504, and 26695 showed that atractylenolide I (<b>1</b>) had potent inhibitory effects against all three strains, with MIC<sub>50</sub> values of ranging from 27.3 to 48.6 μM and MIC<sub>90</sub> values from 45.4 to 87.2 μM. Atractylenolide III (<b>3</b>) exhibited selective activity against strain 51 with MIC<sub>50</sub> value of 89.9 μM. Both compounds also exhibited anti-inflammatory activity with IC<sub>90</sub> values of 23.3 and 31.1 μM, respectively, although they showed little effect on urease. This is the first report on the anti-<i>H. pylori</i> efficacy of various constituents of <i>A. macrocephala</i> and comparative analysis of inhibitory effects against several strains, which will provide scientific evidence supporting its potential as therapeutic agent for <i>H. pylori</i>-related infection.https://www.mdpi.com/1420-3049/30/15/3142<i>Atractylodes macrocephala</i>anti-<i>Helicobacter pylori</i>sesquiterpeneatractylenolide Ianti-inflammatory |
| spellingShingle | So Yeong Jeong Dong-Min Kang Hyun-Jun Kim Sang Won Yeon Hak Hyun Lee Min Hee Kim Bang Yeon Hwang Mi-Jeong Ahn Mi Kyeong Lee Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i> Molecules <i>Atractylodes macrocephala</i> anti-<i>Helicobacter pylori</i> sesquiterpene atractylenolide I anti-inflammatory |
| title | Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i> |
| title_full | Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i> |
| title_fullStr | Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i> |
| title_full_unstemmed | Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i> |
| title_short | Anti-<i>Helicobacter pylori</i> and Anti-Inflammatory Sesquiterpenoids from the Rhizoma of <i>Atractylodes macrocephala</i> |
| title_sort | anti i helicobacter pylori i and anti inflammatory sesquiterpenoids from the rhizoma of i atractylodes macrocephala i |
| topic | <i>Atractylodes macrocephala</i> anti-<i>Helicobacter pylori</i> sesquiterpene atractylenolide I anti-inflammatory |
| url | https://www.mdpi.com/1420-3049/30/15/3142 |
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