Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses
Lactoferrin (LF), a multifunctional glycoprotein found abundantly in bovine colostrum, is known for its regenerative and anti-inflammatory properties. In this study, we investigated the wound healing and immunomodulatory effects of colostrum-derived exosome-encapsulated lactoferrin (EV-exoLF) on der...
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MDPI AG
2025-07-01
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| author | Chu-Hsun Cheng Wei-Jer Hong Chien-Nien Li Yung-Hsueh Huang Jeng-Haw Tsai Chih-Yuan Huang Jen-Chin Wu Chan-Yen Kuo Wen-Chun Kuo |
| author_facet | Chu-Hsun Cheng Wei-Jer Hong Chien-Nien Li Yung-Hsueh Huang Jeng-Haw Tsai Chih-Yuan Huang Jen-Chin Wu Chan-Yen Kuo Wen-Chun Kuo |
| author_sort | Chu-Hsun Cheng |
| collection | DOAJ |
| description | Lactoferrin (LF), a multifunctional glycoprotein found abundantly in bovine colostrum, is known for its regenerative and anti-inflammatory properties. In this study, we investigated the wound healing and immunomodulatory effects of colostrum-derived exosome-encapsulated lactoferrin (EV-exoLF) on dermal fibroblasts. EV-exoLF was isolated and characterized via nanoparticle tracking analysis and flow cytometry. Functional assays demonstrated that EV-exoLF significantly promoted fibroblast viability and migration in both mouse NIH/3T3 and human HS-68 cell lines. Furthermore, EV-exoLF reduced the expression of pro-inflammatory cytokines (IL-1 and IL-6) and phosphorylated JNK in lipopolysaccharide (LPS)-treated fibroblasts. These findings suggest that EV-exoLF not only enhances fibroblast-mediated wound closure but also mitigates inflammation, highlighting its therapeutic potential in skin regeneration. Colostrum-derived exosomal lactoferrin may serve as a promising natural, cell-free strategy for managing inflammatory skin conditions and improving wound healing outcomes. |
| format | Article |
| id | doaj-art-10f60c8e617a46ffb2087f9f3ce276a2 |
| institution | DOAJ |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-10f60c8e617a46ffb2087f9f3ce276a22025-08-20T02:45:45ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-07-0147754910.3390/cimb47070549Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory ResponsesChu-Hsun Cheng0Wei-Jer Hong1Chien-Nien Li2Yung-Hsueh Huang3Jeng-Haw Tsai4Chih-Yuan Huang5Jen-Chin Wu6Chan-Yen Kuo7Wen-Chun Kuo8Bio-METS Biotech Consulting Co., Ltd., New Taipei City 23544, TaiwanJFM Dermatology Clinic, Taipei 104, TaiwanJFM Dermatology Clinic, Taipei 104, Taiwan20 SKIN Clinic, Taichung City 404, TaiwanBeauty Humanity Clinic, New Taipei City 234, TaiwanBuddhist Tzu Chi General Hospital, Taichung Branch, Taichung City 427213, TaiwanWu Jenchin Dermatology Clinic, Pingtung City 813755, TaiwanInstitute of Oral Medicine and Materials, College of Medicine, Tzu Chi University, Hualien 970, TaiwanBio-METS Biotech Consulting Co., Ltd., New Taipei City 23544, TaiwanLactoferrin (LF), a multifunctional glycoprotein found abundantly in bovine colostrum, is known for its regenerative and anti-inflammatory properties. In this study, we investigated the wound healing and immunomodulatory effects of colostrum-derived exosome-encapsulated lactoferrin (EV-exoLF) on dermal fibroblasts. EV-exoLF was isolated and characterized via nanoparticle tracking analysis and flow cytometry. Functional assays demonstrated that EV-exoLF significantly promoted fibroblast viability and migration in both mouse NIH/3T3 and human HS-68 cell lines. Furthermore, EV-exoLF reduced the expression of pro-inflammatory cytokines (IL-1 and IL-6) and phosphorylated JNK in lipopolysaccharide (LPS)-treated fibroblasts. These findings suggest that EV-exoLF not only enhances fibroblast-mediated wound closure but also mitigates inflammation, highlighting its therapeutic potential in skin regeneration. Colostrum-derived exosomal lactoferrin may serve as a promising natural, cell-free strategy for managing inflammatory skin conditions and improving wound healing outcomes.https://www.mdpi.com/1467-3045/47/7/549exosomeslactoferrincolostruminflammationfibroblastswound healing |
| spellingShingle | Chu-Hsun Cheng Wei-Jer Hong Chien-Nien Li Yung-Hsueh Huang Jeng-Haw Tsai Chih-Yuan Huang Jen-Chin Wu Chan-Yen Kuo Wen-Chun Kuo Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses Current Issues in Molecular Biology exosomes lactoferrin colostrum inflammation fibroblasts wound healing |
| title | Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses |
| title_full | Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses |
| title_fullStr | Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses |
| title_full_unstemmed | Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses |
| title_short | Colostrum-Derived Exosomal Lactoferrin Promotes Skin Fibroblast Regeneration by Suppressing Inflammatory Responses |
| title_sort | colostrum derived exosomal lactoferrin promotes skin fibroblast regeneration by suppressing inflammatory responses |
| topic | exosomes lactoferrin colostrum inflammation fibroblasts wound healing |
| url | https://www.mdpi.com/1467-3045/47/7/549 |
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