High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere
Saponins, natural plant compounds exhibiting health benefits, were extracted from B. vulgaris L. cultivar (cv.) Red Sphere and separated by high-speed counter-current chromatography (HSCCC) in a new solvent system composed of: TBME-BuOH-ACN-H2O (1:2:1:5, v/v/v/v ). The system was used in the head...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2020-02-01
|
| Series: | Polish Journal of Food and Nutrition Sciences |
| Subjects: | |
| Online Access: | http://www.journalssystem.com/pjfns/High-speed-counter-current-chromatography-in-separation-r-nand-identification-of,116416,0,2.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573602070790144 |
|---|---|
| author | Aneta Ewa Spórna-Kucab Sławomir Wybraniec |
| author_facet | Aneta Ewa Spórna-Kucab Sławomir Wybraniec |
| author_sort | Aneta Ewa Spórna-Kucab |
| collection | DOAJ |
| description | Saponins, natural plant compounds exhibiting health benefits, were extracted from B. vulgaris L. cultivar (cv.) Red Sphere and separated by high-speed counter-current chromatography (HSCCC) in a new solvent system composed of: TBME-BuOH-ACN-H2O (1:2:1:5, v/v/v/v ). The system was used in the head-to-tail mode. The flow rate of the mobile phase was 3 mL/min and the column rotation speed was 860 rpm. The retention of the stationary phase was 47%. The LC-MS/MS analyses were performed for the identification of separated saponins in the crude extract as well as HSCCC fractions,.
Significantly different elution orders of the analytes were observed in the HSCCC and HPLC systems, indicating the complementarity of both the techniques in the fractionation of saponins. Moreover, during the HSCCC separation, acetal-type saponins were eluted faster than pentose/hexose-type saponins and then dioxolane-type saponins. The separation of saponins in the HSCCC solvent system enabled the fractionation and preconcentration of 13 saponins for further fragmentation experiments in the LC-MS/MS system. Nine saponins were detected for the first time ever in B. vulgaris L. cv. Red Sphere. |
| format | Article |
| id | doaj-art-10f0981a35f84e148532eb831c42b49d |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2020-02-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-10f0981a35f84e148532eb831c42b49d2025-02-02T03:35:46ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072020-02-01701677410.31883/pjfns/116416116416High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red SphereAneta Ewa Spórna-Kucab0Sławomir Wybraniec1Department of Analytical Chemistry, Institute C-1, Cracow University of Technology, Faculty of Chemical Engineering and Technology, Warszawska Str. 24, 31-155, Cracow, PolandDepartment of Analytical Chemistry, Institute C-1, Cracow University of Technology, Faculty of Chemical Engineering and Technology, Warszawska Str. 24, 31-155, Cracow, PolandSaponins, natural plant compounds exhibiting health benefits, were extracted from B. vulgaris L. cultivar (cv.) Red Sphere and separated by high-speed counter-current chromatography (HSCCC) in a new solvent system composed of: TBME-BuOH-ACN-H2O (1:2:1:5, v/v/v/v ). The system was used in the head-to-tail mode. The flow rate of the mobile phase was 3 mL/min and the column rotation speed was 860 rpm. The retention of the stationary phase was 47%. The LC-MS/MS analyses were performed for the identification of separated saponins in the crude extract as well as HSCCC fractions,. Significantly different elution orders of the analytes were observed in the HSCCC and HPLC systems, indicating the complementarity of both the techniques in the fractionation of saponins. Moreover, during the HSCCC separation, acetal-type saponins were eluted faster than pentose/hexose-type saponins and then dioxolane-type saponins. The separation of saponins in the HSCCC solvent system enabled the fractionation and preconcentration of 13 saponins for further fragmentation experiments in the LC-MS/MS system. Nine saponins were detected for the first time ever in B. vulgaris L. cv. Red Sphere.http://www.journalssystem.com/pjfns/High-speed-counter-current-chromatography-in-separation-r-nand-identification-of,116416,0,2.htmlsaponinsbeta vulgaris lhigh-speed counter-current chromatography |
| spellingShingle | Aneta Ewa Spórna-Kucab Sławomir Wybraniec High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere Polish Journal of Food and Nutrition Sciences saponins beta vulgaris l high-speed counter-current chromatography |
| title | High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere |
| title_full | High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere |
| title_fullStr | High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere |
| title_full_unstemmed | High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere |
| title_short | High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere |
| title_sort | high speed counter current chromatography in separation and identification of saponins from beta vulgaris l cultivar red sphere |
| topic | saponins beta vulgaris l high-speed counter-current chromatography |
| url | http://www.journalssystem.com/pjfns/High-speed-counter-current-chromatography-in-separation-r-nand-identification-of,116416,0,2.html |
| work_keys_str_mv | AT anetaewaspornakucab highspeedcountercurrentchromatographyinseparationandidentificationofsaponinsfrombetavulgarislcultivarredsphere AT sławomirwybraniec highspeedcountercurrentchromatographyinseparationandidentificationofsaponinsfrombetavulgarislcultivarredsphere |