New insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analyses
Abstract Background As an important prokaryotic model organism, Bacillus subtilis has been widely used in the industrial production of a variety of target products. The efficient secretion of target products has always been the main purpose of industrial microbial technology. The modification of gen...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-024-03700-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850086108318138368 |
|---|---|
| author | Huabiao Miao Xia Xiang Ling Cheng Qian Wu Zunxi Huang |
| author_facet | Huabiao Miao Xia Xiang Ling Cheng Qian Wu Zunxi Huang |
| author_sort | Huabiao Miao |
| collection | DOAJ |
| description | Abstract Background As an important prokaryotic model organism, Bacillus subtilis has been widely used in the industrial production of a variety of target products. The efficient secretion of target products has always been the main purpose of industrial microbial technology. The modification of gene regulatory networks is an important technical means to construct a factory of microbial cells that efficiently secretes target products. However, the regulatory network of the efficient expression of foreign genes in B. subtilis has not been studied at the translation level. Results In this study, Ribo-seq and RNA-seq technology were used to study the changes in differentially expressed genes during the efficient secretion of the protease PB92 by B. subtilis WB600, and the results revealed the gene regulatory network related to efficient secretion of foreign protein. The results revealed that the correlation between the differentially expressed genes of B. subtilis at the transcription and translation levels was only 0.5354. Forty-one common (transcription and translation) and 436 unique (translation) key differential gene sets that may be related to the efficient secretion of foreign proteins were revealed. KEGG enrichment analysis of these key gene sets revealed that they were involved mainly in the cell motility and central metabolic regulatory network of B. subtilis. Conclusion Our study provides important guidance for the construction of cell factories and metabolic networks for the efficient secretion of target products by B. subtilis. |
| format | Article |
| id | doaj-art-10e2b0406412414c81db77dedd67e356 |
| institution | DOAJ |
| issn | 1471-2180 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj-art-10e2b0406412414c81db77dedd67e3562025-08-20T02:43:35ZengBMCBMC Microbiology1471-21802024-12-0124111510.1186/s12866-024-03700-yNew insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analysesHuabiao Miao0Xia Xiang1Ling Cheng2Qian Wu3Zunxi Huang4School of Life Science, Yunnan Normal UniversitySchool of Life Science, Yunnan Normal UniversityGuangzhou Genedenovo Biotechnology Company LimitedSchool of Life Science, Yunnan Normal UniversitySchool of Life Science, Yunnan Normal UniversityAbstract Background As an important prokaryotic model organism, Bacillus subtilis has been widely used in the industrial production of a variety of target products. The efficient secretion of target products has always been the main purpose of industrial microbial technology. The modification of gene regulatory networks is an important technical means to construct a factory of microbial cells that efficiently secretes target products. However, the regulatory network of the efficient expression of foreign genes in B. subtilis has not been studied at the translation level. Results In this study, Ribo-seq and RNA-seq technology were used to study the changes in differentially expressed genes during the efficient secretion of the protease PB92 by B. subtilis WB600, and the results revealed the gene regulatory network related to efficient secretion of foreign protein. The results revealed that the correlation between the differentially expressed genes of B. subtilis at the transcription and translation levels was only 0.5354. Forty-one common (transcription and translation) and 436 unique (translation) key differential gene sets that may be related to the efficient secretion of foreign proteins were revealed. KEGG enrichment analysis of these key gene sets revealed that they were involved mainly in the cell motility and central metabolic regulatory network of B. subtilis. Conclusion Our study provides important guidance for the construction of cell factories and metabolic networks for the efficient secretion of target products by B. subtilis.https://doi.org/10.1186/s12866-024-03700-yBacillus subtilisRibosome profilingRNA sequencingForeign proteinGene regulatory networks |
| spellingShingle | Huabiao Miao Xia Xiang Ling Cheng Qian Wu Zunxi Huang New insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analyses BMC Microbiology Bacillus subtilis Ribosome profiling RNA sequencing Foreign protein Gene regulatory networks |
| title | New insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analyses |
| title_full | New insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analyses |
| title_fullStr | New insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analyses |
| title_full_unstemmed | New insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analyses |
| title_short | New insights into the efficient secretion of foreign protein in Bacillus subtilis via Ribo-seq and RNA-seq integrative analyses |
| title_sort | new insights into the efficient secretion of foreign protein in bacillus subtilis via ribo seq and rna seq integrative analyses |
| topic | Bacillus subtilis Ribosome profiling RNA sequencing Foreign protein Gene regulatory networks |
| url | https://doi.org/10.1186/s12866-024-03700-y |
| work_keys_str_mv | AT huabiaomiao newinsightsintotheefficientsecretionofforeignproteininbacillussubtilisviariboseqandrnaseqintegrativeanalyses AT xiaxiang newinsightsintotheefficientsecretionofforeignproteininbacillussubtilisviariboseqandrnaseqintegrativeanalyses AT lingcheng newinsightsintotheefficientsecretionofforeignproteininbacillussubtilisviariboseqandrnaseqintegrativeanalyses AT qianwu newinsightsintotheefficientsecretionofforeignproteininbacillussubtilisviariboseqandrnaseqintegrativeanalyses AT zunxihuang newinsightsintotheefficientsecretionofforeignproteininbacillussubtilisviariboseqandrnaseqintegrativeanalyses |