The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal artery
The current study represents the first attempt to investigate the effect of Adiantum capillus-veneris extract (ACVE) on goat’s isolated renal artery smooth muscle’s cell. Therefore, this research aims to investigate the possible action of Adiantum capillus-veneris extract (ACVE) (1×10-4–10-9 mg/ml)...
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Erbil Polytechnic University
2023-09-01
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| Series: | Polytechnic Journal |
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| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol12/iss1/9 |
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| author | Aveen Muhsen Asaad Ismail Salih Ibraheem |
| author_facet | Aveen Muhsen Asaad Ismail Salih Ibraheem |
| author_sort | Aveen Muhsen Asaad |
| collection | DOAJ |
| description | The current study represents the first attempt to investigate the effect of Adiantum capillus-veneris extract
(ACVE) on goat’s isolated renal artery smooth muscle’s cell. Therefore, this research aims to investigate the
possible action of Adiantum capillus-veneris extract (ACVE) (1×10-4–10-9 mg/ml) in the vascular activity of goat
isolated renal artery by using the organ bath and PowerLab data acquisition system, and the phytochemical
analysis for the ACVE extract was done by using gas chromatography-mass spectrometry (GC/MS).
The results of the current study showed that ACVE caused concentration-dependent relaxation of endothelium
intact renal artery rings precontracted with a high level of KCl (60 mM) or phenylephrine (PE) (10-5 M), also they
are exhibited potent inhibitory effects on PE, and less potent on KCl-induced contractions.
Renal artery rings preincubated with potassium (K+) channel blocker (tetraethylammonium, TEA), barium chloride
(BaCl2), 4-aminopyridine (4-AP), (indomethacin) and (clotrimazole) showed a significant effect in renal artery
smooth muscle relaxation induced by ACVE. While glibenclamide (Glib) and L-NAME did not exhibit any role in
the relaxation effect of ACVE.
Furthermore, the role of nifedipine L-type calcium channels blocker in the effects of ACVE suggests that a Ca2+
channel blocking mechanism has a relaxant effect in the goat’s renal artery smooth muscles. Renal artery rings
preincubated with nifedipine (1×10-5 and 3×10-5
), and ACVE (1×10-5 and 3×10-5
), produced a potent inhibitory
effect on CaCl2 induced contraction as compared with the control group, and also reduced the maximum
contraction.
Results of the current study, it can be concluded that ACVE have potent vasorelaxation effects on renal artery
rings which are mediated, partly, by the enhancement of PGI2, EET and modulating different K+ channels and Ltype Ca2+ channels activities. Additionally, the results of the current study provide the mechanism of action of
some constituents of the medicinal plant Adiantum capillus-veneris which can be exploited to develop more
specific drugs to be used for the treatment of various vascular diseases based on phytochemical analysis of the
extract. |
| format | Article |
| id | doaj-art-10e10635b1f340c3afeaebbbf79e55e0 |
| institution | OA Journals |
| issn | 2707-7799 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Erbil Polytechnic University |
| record_format | Article |
| series | Polytechnic Journal |
| spelling | doaj-art-10e10635b1f340c3afeaebbbf79e55e02025-08-20T02:02:16ZengErbil Polytechnic UniversityPolytechnic Journal2707-77992023-09-011216778https://doi.org/10.25156/ptj.v12n1y2022.pp67-78The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal arteryAveen Muhsen Asaad0Ismail Salih Ibraheem1Department of Medical Laboratory Technology, Koya Technical Institute, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq.Department of Biology, Faculty of Science and Health, Koya University, Erbil, Kurdistan Region, Iraq. The current study represents the first attempt to investigate the effect of Adiantum capillus-veneris extract (ACVE) on goat’s isolated renal artery smooth muscle’s cell. Therefore, this research aims to investigate the possible action of Adiantum capillus-veneris extract (ACVE) (1×10-4–10-9 mg/ml) in the vascular activity of goat isolated renal artery by using the organ bath and PowerLab data acquisition system, and the phytochemical analysis for the ACVE extract was done by using gas chromatography-mass spectrometry (GC/MS). The results of the current study showed that ACVE caused concentration-dependent relaxation of endothelium intact renal artery rings precontracted with a high level of KCl (60 mM) or phenylephrine (PE) (10-5 M), also they are exhibited potent inhibitory effects on PE, and less potent on KCl-induced contractions. Renal artery rings preincubated with potassium (K+) channel blocker (tetraethylammonium, TEA), barium chloride (BaCl2), 4-aminopyridine (4-AP), (indomethacin) and (clotrimazole) showed a significant effect in renal artery smooth muscle relaxation induced by ACVE. While glibenclamide (Glib) and L-NAME did not exhibit any role in the relaxation effect of ACVE. Furthermore, the role of nifedipine L-type calcium channels blocker in the effects of ACVE suggests that a Ca2+ channel blocking mechanism has a relaxant effect in the goat’s renal artery smooth muscles. Renal artery rings preincubated with nifedipine (1×10-5 and 3×10-5 ), and ACVE (1×10-5 and 3×10-5 ), produced a potent inhibitory effect on CaCl2 induced contraction as compared with the control group, and also reduced the maximum contraction. Results of the current study, it can be concluded that ACVE have potent vasorelaxation effects on renal artery rings which are mediated, partly, by the enhancement of PGI2, EET and modulating different K+ channels and Ltype Ca2+ channels activities. Additionally, the results of the current study provide the mechanism of action of some constituents of the medicinal plant Adiantum capillus-veneris which can be exploited to develop more specific drugs to be used for the treatment of various vascular diseases based on phytochemical analysis of the extract. https://polytechnic-journal.epu.edu.iq/home/vol12/iss1/9adiantum capillus,smooth muscle relaxation,renal artery,k+ channels blocker,ca2+channelsblocker. |
| spellingShingle | Aveen Muhsen Asaad Ismail Salih Ibraheem The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal artery Polytechnic Journal adiantum capillus, smooth muscle relaxation, renal artery, k+ channels blocker, ca2+channels blocker. |
| title | The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal artery |
| title_full | The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal artery |
| title_fullStr | The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal artery |
| title_full_unstemmed | The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal artery |
| title_short | The vasorelaxant effect of Adiantum capillus-veneries extract in the vascular activity of goat’s isolated renal artery |
| title_sort | vasorelaxant effect of adiantum capillus veneries extract in the vascular activity of goat s isolated renal artery |
| topic | adiantum capillus, smooth muscle relaxation, renal artery, k+ channels blocker, ca2+channels blocker. |
| url | https://polytechnic-journal.epu.edu.iq/home/vol12/iss1/9 |
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