Gut commensal microbiota drive tailored macrophage responses
Summary: Macrophages serve as sentinels at the intestinal surface, responding to organismal cues to drive proinflammatory or tolerogenic responses. To date, studies of combinations of these cues do not fully capture the heterogeneity of macrophage responses. To address this gap, we performed multipl...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725009283 |
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| author | Josh Jones Karla Y. García-Martínez Yi-Yuan Lee Matt Rhee Rahul R. Nath Sola Takahashi Benjamin Grodner Iwijn De Vlaminck Cynthia A. Leifer Ilana L. Brito |
| author_facet | Josh Jones Karla Y. García-Martínez Yi-Yuan Lee Matt Rhee Rahul R. Nath Sola Takahashi Benjamin Grodner Iwijn De Vlaminck Cynthia A. Leifer Ilana L. Brito |
| author_sort | Josh Jones |
| collection | DOAJ |
| description | Summary: Macrophages serve as sentinels at the intestinal surface, responding to organismal cues to drive proinflammatory or tolerogenic responses. To date, studies of combinations of these cues do not fully capture the heterogeneity of macrophage responses. To address this gap, we performed multiplexed single-cell RNA sequencing on 74,476 human monocyte–derived macrophages following exposure to 15 bacteria, mostly commensals. We observe clusters that appeared only after macrophage exposure to bacteria, and transcriptional responses within each cluster varied by species and Gram status. The proportion of each cluster also varied among exposure conditions. Macrophages exposed to defined combinations of organisms revealed that Fusobacterium nucleatum drives inflammatory responses, whereas Mediterraneibacter gnavus tempers them. Overall, our results show that macrophages distinguish between commensal organisms, relevant to intestinal diseases characterized by altered microbiome compositions. This sequencing dataset will be a useful resource to probe human macrophage response to a broad range of bacteria. |
| format | Article |
| id | doaj-art-10ddeda87f524836aba0ef521e297e40 |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-10ddeda87f524836aba0ef521e297e402025-08-20T05:06:24ZengElsevierCell Reports2211-12472025-08-0144811615710.1016/j.celrep.2025.116157Gut commensal microbiota drive tailored macrophage responsesJosh Jones0Karla Y. García-Martínez1Yi-Yuan Lee2Matt Rhee3Rahul R. Nath4Sola Takahashi5Benjamin Grodner6Iwijn De Vlaminck7Cynthia A. Leifer8Ilana L. Brito9Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USACollege of Veterinary Medicine, Cornell University, Ithaca, NY, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USAMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USACollege of Veterinary Medicine, Cornell University, Ithaca, NY, USA; Corresponding authorMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY, USA; Corresponding authorSummary: Macrophages serve as sentinels at the intestinal surface, responding to organismal cues to drive proinflammatory or tolerogenic responses. To date, studies of combinations of these cues do not fully capture the heterogeneity of macrophage responses. To address this gap, we performed multiplexed single-cell RNA sequencing on 74,476 human monocyte–derived macrophages following exposure to 15 bacteria, mostly commensals. We observe clusters that appeared only after macrophage exposure to bacteria, and transcriptional responses within each cluster varied by species and Gram status. The proportion of each cluster also varied among exposure conditions. Macrophages exposed to defined combinations of organisms revealed that Fusobacterium nucleatum drives inflammatory responses, whereas Mediterraneibacter gnavus tempers them. Overall, our results show that macrophages distinguish between commensal organisms, relevant to intestinal diseases characterized by altered microbiome compositions. This sequencing dataset will be a useful resource to probe human macrophage response to a broad range of bacteria.http://www.sciencedirect.com/science/article/pii/S2211124725009283CP: ImmunologyCP: Microbiology |
| spellingShingle | Josh Jones Karla Y. García-Martínez Yi-Yuan Lee Matt Rhee Rahul R. Nath Sola Takahashi Benjamin Grodner Iwijn De Vlaminck Cynthia A. Leifer Ilana L. Brito Gut commensal microbiota drive tailored macrophage responses Cell Reports CP: Immunology CP: Microbiology |
| title | Gut commensal microbiota drive tailored macrophage responses |
| title_full | Gut commensal microbiota drive tailored macrophage responses |
| title_fullStr | Gut commensal microbiota drive tailored macrophage responses |
| title_full_unstemmed | Gut commensal microbiota drive tailored macrophage responses |
| title_short | Gut commensal microbiota drive tailored macrophage responses |
| title_sort | gut commensal microbiota drive tailored macrophage responses |
| topic | CP: Immunology CP: Microbiology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725009283 |
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