Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying Hens
Carotenoids from different sources have different structures and functions, and their dietary components benefit the health of various organisms. The effects of halophilic Archaea-derived C50 carotenoids on poultry egg quality and gut microbiota remain largely unexplored. In this study, we isolated...
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2024-12-01
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| author | Xufeng Dou Guodong Zhang Hao Tang Xiaoxue Chen Beibei Chen Yuxia Mei Haihong Jiao Min Ren |
| author_facet | Xufeng Dou Guodong Zhang Hao Tang Xiaoxue Chen Beibei Chen Yuxia Mei Haihong Jiao Min Ren |
| author_sort | Xufeng Dou |
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| description | Carotenoids from different sources have different structures and functions, and their dietary components benefit the health of various organisms. The effects of halophilic Archaea-derived C50 carotenoids on poultry egg quality and gut microbiota remain largely unexplored. In this study, we isolated a carotenoid-secreting strain of <i>Halalkalicoccus paucihalophilus</i>, TRM89021, from the Pamir Plateau. We characterized the carotenoid pigments produced by this strain; the major components were bacterioruberin and its derivatives. The effects of these carotenoids on the egg quality and cecal microbiota composition of hens were investigated. Compared to the basal diet group (BDG), supplementation with carotenoids in the carotenoids-supplemented diet group (CDG) resulted in significantly lower a* and b* scores at week 5 and lower b* scores and Haugh units at week 2, while egg strength and weight were higher. CDG also showed increased yolk antioxidant capacity, higher glutathione peroxidase levels, and significantly lower catalase levels (<i>p</i> < 0.05). Plasma analysis revealed elevated total bilirubin and aspartate aminotransferase levels, along with reduced inorganic phosphorus levels in the CDG (<i>p</i> < 0.05). No significant differences in cecal microbiota diversity were observed between the groups at any taxonomic level. This result suggests that halophilic archaea-derived carotenoids have the potential to be used as natural feed supplements to improve egg quality. Our study provides a theoretical basis for applying archaea-derived carotenoids in poultry diets. |
| format | Article |
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| issn | 2076-2615 |
| language | English |
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| spelling | doaj-art-10c445151a634644a91052b025ea19e62025-08-20T02:38:35ZengMDPI AGAnimals2076-26152024-12-011423347010.3390/ani14233470Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying HensXufeng Dou0Guodong Zhang1Hao Tang2Xiaoxue Chen3Beibei Chen4Yuxia Mei5Haihong Jiao6Min Ren7Key Laboratory of Conservation and Utilization of Biological Resources in the Tarim Basin, Alar 843300, ChinaCollege of Animal Science and Technology, Tarim University, Alar 843300, ChinaKey Laboratory of Conservation and Utilization of Biological Resources in the Tarim Basin, Alar 843300, ChinaCollege of Animal Science and Technology, Tarim University, Alar 843300, ChinaCollege of Animal Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430000, ChinaCollege of Animal Science and Technology, Tarim University, Alar 843300, ChinaKey Laboratory of Conservation and Utilization of Biological Resources in the Tarim Basin, Alar 843300, ChinaCarotenoids from different sources have different structures and functions, and their dietary components benefit the health of various organisms. The effects of halophilic Archaea-derived C50 carotenoids on poultry egg quality and gut microbiota remain largely unexplored. In this study, we isolated a carotenoid-secreting strain of <i>Halalkalicoccus paucihalophilus</i>, TRM89021, from the Pamir Plateau. We characterized the carotenoid pigments produced by this strain; the major components were bacterioruberin and its derivatives. The effects of these carotenoids on the egg quality and cecal microbiota composition of hens were investigated. Compared to the basal diet group (BDG), supplementation with carotenoids in the carotenoids-supplemented diet group (CDG) resulted in significantly lower a* and b* scores at week 5 and lower b* scores and Haugh units at week 2, while egg strength and weight were higher. CDG also showed increased yolk antioxidant capacity, higher glutathione peroxidase levels, and significantly lower catalase levels (<i>p</i> < 0.05). Plasma analysis revealed elevated total bilirubin and aspartate aminotransferase levels, along with reduced inorganic phosphorus levels in the CDG (<i>p</i> < 0.05). No significant differences in cecal microbiota diversity were observed between the groups at any taxonomic level. This result suggests that halophilic archaea-derived carotenoids have the potential to be used as natural feed supplements to improve egg quality. Our study provides a theoretical basis for applying archaea-derived carotenoids in poultry diets.https://www.mdpi.com/2076-2615/14/23/3470carotenoidsegg quality<i>Halalkalicoccus paucihalophilus</i>antioxidant capacitycecal microbiota |
| spellingShingle | Xufeng Dou Guodong Zhang Hao Tang Xiaoxue Chen Beibei Chen Yuxia Mei Haihong Jiao Min Ren Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying Hens Animals carotenoids egg quality <i>Halalkalicoccus paucihalophilus</i> antioxidant capacity cecal microbiota |
| title | Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying Hens |
| title_full | Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying Hens |
| title_fullStr | Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying Hens |
| title_full_unstemmed | Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying Hens |
| title_short | Carotenoids from Halophilic Archaea: A Novel Approach to Improve Egg Quality and Cecal Microbiota in Laying Hens |
| title_sort | carotenoids from halophilic archaea a novel approach to improve egg quality and cecal microbiota in laying hens |
| topic | carotenoids egg quality <i>Halalkalicoccus paucihalophilus</i> antioxidant capacity cecal microbiota |
| url | https://www.mdpi.com/2076-2615/14/23/3470 |
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