Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer
Abstract DNA methylation is mediated by DNA methyltransferases (DNMTs), and the stability of their activity is essential for cellular fate. DNMT1 is considered one of the most promising targets for research. However, current detection techniques are limited in accurately quantifying its activity in...
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| Format: | Article |
| Language: | English |
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Wiley
2025-08-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202501064 |
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| author | Yingran Wang Guozhi Zhang Zhizhao Zhang Mengsi Zhang Jiao Chen Ke Wang Lu Liu Jing Bao Ming Chen Xiaowei Qi Mingxuan Gao |
| author_facet | Yingran Wang Guozhi Zhang Zhizhao Zhang Mengsi Zhang Jiao Chen Ke Wang Lu Liu Jing Bao Ming Chen Xiaowei Qi Mingxuan Gao |
| author_sort | Yingran Wang |
| collection | DOAJ |
| description | Abstract DNA methylation is mediated by DNA methyltransferases (DNMTs), and the stability of their activity is essential for cellular fate. DNMT1 is considered one of the most promising targets for research. However, current detection techniques are limited in accurately quantifying its activity in peripheral blood. Here, a reaction system is developed known as DNMT1 Identification by Variable Activity (DIVA) for the highly sensitive detection of DNMT1 activity in the peripheral blood of breast cancer patients. DIVA can detect DNMT1 at levels as low as 10−7 U mL−1, with minimal time and cost. This method is applied to analyze 271 clinical samples, successfully evaluating tumor burden in patients staged I‐IV. Finally, this method is utilized to assess the prognosis of 22 patients undergoing neoadjuvant therapy, demonstrating good consistency with ultrasound imaging results. It is believed that DIVA could serve as an effective auxiliary technique for both the early detection of breast cancer and evaluation of neoadjuvant therapy. |
| format | Article |
| id | doaj-art-109bc075893b4a19b3de8859d9604ff1 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-109bc075893b4a19b3de8859d9604ff12025-08-23T14:14:32ZengWileyAdvanced Science2198-38442025-08-011231n/an/a10.1002/advs.202501064Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast CancerYingran Wang0Guozhi Zhang1Zhizhao Zhang2Mengsi Zhang3Jiao Chen4Ke Wang5Lu Liu6Jing Bao7Ming Chen8Xiaowei Qi9Mingxuan Gao10Department of Clinical Laboratory Medicine Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Breast and Thyroid Surgery Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Breast and Thyroid Surgery Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaInstitute of Pathology and Southwest Cancer Center Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Clinical Laboratory Medicine Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Clinical Laboratory Medicine Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Clinical Laboratory Medicine Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Clinical Laboratory Medicine Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Clinical Laboratory Medicine Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Breast and Thyroid Surgery Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaDepartment of Clinical Laboratory Medicine Southwest Hospital Third Military Medical University (Army Medical University) Chongqing 400038 P. R. ChinaAbstract DNA methylation is mediated by DNA methyltransferases (DNMTs), and the stability of their activity is essential for cellular fate. DNMT1 is considered one of the most promising targets for research. However, current detection techniques are limited in accurately quantifying its activity in peripheral blood. Here, a reaction system is developed known as DNMT1 Identification by Variable Activity (DIVA) for the highly sensitive detection of DNMT1 activity in the peripheral blood of breast cancer patients. DIVA can detect DNMT1 at levels as low as 10−7 U mL−1, with minimal time and cost. This method is applied to analyze 271 clinical samples, successfully evaluating tumor burden in patients staged I‐IV. Finally, this method is utilized to assess the prognosis of 22 patients undergoing neoadjuvant therapy, demonstrating good consistency with ultrasound imaging results. It is believed that DIVA could serve as an effective auxiliary technique for both the early detection of breast cancer and evaluation of neoadjuvant therapy.https://doi.org/10.1002/advs.202501064breast cancermodularized tandem reactionneoadjuvant therapy response predictionplasma DNMT1tumor burden assessment |
| spellingShingle | Yingran Wang Guozhi Zhang Zhizhao Zhang Mengsi Zhang Jiao Chen Ke Wang Lu Liu Jing Bao Ming Chen Xiaowei Qi Mingxuan Gao Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer Advanced Science breast cancer modularized tandem reaction neoadjuvant therapy response prediction plasma DNMT1 tumor burden assessment |
| title | Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer |
| title_full | Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer |
| title_fullStr | Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer |
| title_full_unstemmed | Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer |
| title_short | Plasma DNMT1 Activity for Assessing Tumor Burden and Predicting Neoadjuvant Therapy Response in Breast Cancer |
| title_sort | plasma dnmt1 activity for assessing tumor burden and predicting neoadjuvant therapy response in breast cancer |
| topic | breast cancer modularized tandem reaction neoadjuvant therapy response prediction plasma DNMT1 tumor burden assessment |
| url | https://doi.org/10.1002/advs.202501064 |
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