Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings
Objective: Evaluate the dentinogenesis in the offspring of rats submitted to gestational protein restriction (GPR). Design: The offspring were evaluated at the 21st day of gestation (21 dG). Assessments were made of morphological parameters and the RANKL/OPG system - bone tissue maturation markers -...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426820301512 |
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| author | Bruno Calsa Beatriz Calloni Masiero Marcelo Augusto Marretto Esquisatto Rosana Catisti Milton Santamaria, Jr. |
| author_facet | Bruno Calsa Beatriz Calloni Masiero Marcelo Augusto Marretto Esquisatto Rosana Catisti Milton Santamaria, Jr. |
| author_sort | Bruno Calsa |
| collection | DOAJ |
| description | Objective: Evaluate the dentinogenesis in the offspring of rats submitted to gestational protein restriction (GPR). Design: The offspring were evaluated at the 21st day of gestation (21 dG). Assessments were made of morphological parameters and the RANKL/OPG system - bone tissue maturation markers - in the upper incisor tooth germ. Pregnant 10-week-old female Wistar rats were divided into normal protein (NP, 17% casein, n = 5) and low protein (LP, 6% casein, n = 5) diet groups. At 21 dG, the offspring maxillae were collected for histomorphometric and immunohistochemical analyses. Results: The LP group showed decreased thickness of the dentin and odontoblast cell layers on the tooth germ. GPR led to decreased OPG expression and increased RANKL expression in the incisor germ. Conclusion: The results suggested that gestational protein restriction altered odontoblast RANKL/OPG expression and decreased dentin matrix deposition and thickness in tooth development. |
| format | Article |
| id | doaj-art-1098d0cbb4e745da8bac49b8d09dfa86 |
| institution | DOAJ |
| issn | 2212-4268 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-1098d0cbb4e745da8bac49b8d09dfa862025-08-20T02:50:25ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682020-10-0110474374610.1016/j.jobcr.2020.10.007Gestational protein restriction alters the RANKL/OPG system in the dental germ of offspringsBruno Calsa0Beatriz Calloni Masiero1Marcelo Augusto Marretto Esquisatto2Rosana Catisti3Milton Santamaria, Jr.4Graduate Program in Biomedical Sciences, University Center of Hermínio Ometto Foundation - FHO, Araras, SP, BrazilGraduate Program in Biomedical Sciences, University Center of Hermínio Ometto Foundation - FHO, Araras, SP, BrazilGraduate Program in Biomedical Sciences, University Center of Hermínio Ometto Foundation - FHO, Araras, SP, BrazilGraduate Program in Biomedical Sciences, University Center of Hermínio Ometto Foundation - FHO, Araras, SP, BrazilGraduate Program in Biomedical Sciences, University Center of Hermínio Ometto Foundation - FHO, Araras, SP, Brazil; Graduate Program in Orthodontics, University Center of Hermínio Ometto Foundation, Araras, SP, Brazil; Corresponding author. Av. Maximiliano Baruto, 500, Postcode - 13607-339, Araras, SP, Brazil.Objective: Evaluate the dentinogenesis in the offspring of rats submitted to gestational protein restriction (GPR). Design: The offspring were evaluated at the 21st day of gestation (21 dG). Assessments were made of morphological parameters and the RANKL/OPG system - bone tissue maturation markers - in the upper incisor tooth germ. Pregnant 10-week-old female Wistar rats were divided into normal protein (NP, 17% casein, n = 5) and low protein (LP, 6% casein, n = 5) diet groups. At 21 dG, the offspring maxillae were collected for histomorphometric and immunohistochemical analyses. Results: The LP group showed decreased thickness of the dentin and odontoblast cell layers on the tooth germ. GPR led to decreased OPG expression and increased RANKL expression in the incisor germ. Conclusion: The results suggested that gestational protein restriction altered odontoblast RANKL/OPG expression and decreased dentin matrix deposition and thickness in tooth development.http://www.sciencedirect.com/science/article/pii/S2212426820301512OdontogenesisDentinogenesisGestational protein restrictionRANKLOsteoprotegerinDental development |
| spellingShingle | Bruno Calsa Beatriz Calloni Masiero Marcelo Augusto Marretto Esquisatto Rosana Catisti Milton Santamaria, Jr. Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings Journal of Oral Biology and Craniofacial Research Odontogenesis Dentinogenesis Gestational protein restriction RANKL Osteoprotegerin Dental development |
| title | Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings |
| title_full | Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings |
| title_fullStr | Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings |
| title_full_unstemmed | Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings |
| title_short | Gestational protein restriction alters the RANKL/OPG system in the dental germ of offsprings |
| title_sort | gestational protein restriction alters the rankl opg system in the dental germ of offsprings |
| topic | Odontogenesis Dentinogenesis Gestational protein restriction RANKL Osteoprotegerin Dental development |
| url | http://www.sciencedirect.com/science/article/pii/S2212426820301512 |
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