Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing
Abstract Procambarus clarkii is one of the important economic species in China and has been served as tasty food in recent years after being introduced to Nanjing. Significant problems of environment factors, such as salinity, pH and temperature, especially salinity, has the potential to result in s...
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ELS Publishing (ELSP)
2019-11-01
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| Series: | ExRNA |
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| Online Access: | http://link.springer.com/article/10.1186/s41544-019-0030-0 |
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| author | Fangfang Jin Yuling Sun |
| author_facet | Fangfang Jin Yuling Sun |
| author_sort | Fangfang Jin |
| collection | DOAJ |
| description | Abstract Procambarus clarkii is one of the important economic species in China and has been served as tasty food in recent years after being introduced to Nanjing. Significant problems of environment factors, such as salinity, pH and temperature, especially salinity, has the potential to result in significant economic losses in many crayfish-producing farms in China. miRNAs are a kind of ~ 22 nucleotide small non coding RNAs which were encoded by plants, animals and some viruses with functions in RNA silencing or post-transcription regulation. We constructed four sRNA library of P. clarkia from different tissues and treatments by using high-throughput sequencing technology. A total of 101 conserved miRNAs and two novel pre-miRNAs were identified and RT-qPCR were further performed to confirm existence of part of identified miRNAs. A genome wide expression profile of salt-tolerance miRNAs was proved and three miRNAs were further validated by RT-qPCR with dynamic response to different salinity stages. The study of miRNAs in P. clarkia can help us better understanding the role of miRNAs in salt-tolerance in P. clarkia. |
| format | Article |
| id | doaj-art-108c54d99f9d49ea8661df08ac3fa375 |
| institution | DOAJ |
| issn | 2398-0060 |
| language | English |
| publishDate | 2019-11-01 |
| publisher | ELS Publishing (ELSP) |
| record_format | Article |
| series | ExRNA |
| spelling | doaj-art-108c54d99f9d49ea8661df08ac3fa3752025-08-20T03:16:29ZengELS Publishing (ELSP)ExRNA2398-00602019-11-01111810.1186/s41544-019-0030-0Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencingFangfang Jin0Yuling Sun1State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing UniversityJiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal UniversityAbstract Procambarus clarkii is one of the important economic species in China and has been served as tasty food in recent years after being introduced to Nanjing. Significant problems of environment factors, such as salinity, pH and temperature, especially salinity, has the potential to result in significant economic losses in many crayfish-producing farms in China. miRNAs are a kind of ~ 22 nucleotide small non coding RNAs which were encoded by plants, animals and some viruses with functions in RNA silencing or post-transcription regulation. We constructed four sRNA library of P. clarkia from different tissues and treatments by using high-throughput sequencing technology. A total of 101 conserved miRNAs and two novel pre-miRNAs were identified and RT-qPCR were further performed to confirm existence of part of identified miRNAs. A genome wide expression profile of salt-tolerance miRNAs was proved and three miRNAs were further validated by RT-qPCR with dynamic response to different salinity stages. The study of miRNAs in P. clarkia can help us better understanding the role of miRNAs in salt-tolerance in P. clarkia.http://link.springer.com/article/10.1186/s41544-019-0030-0miRNACrayfishProcambarus clarkiaHigh-throughput sequencingSalt tolerance |
| spellingShingle | Fangfang Jin Yuling Sun Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing ExRNA miRNA Crayfish Procambarus clarkia High-throughput sequencing Salt tolerance |
| title | Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing |
| title_full | Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing |
| title_fullStr | Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing |
| title_full_unstemmed | Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing |
| title_short | Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing |
| title_sort | identification and characterization of salt tolerance relative mirnas in procambarus clarkii by high throughput sequencing |
| topic | miRNA Crayfish Procambarus clarkia High-throughput sequencing Salt tolerance |
| url | http://link.springer.com/article/10.1186/s41544-019-0030-0 |
| work_keys_str_mv | AT fangfangjin identificationandcharacterizationofsalttolerancerelativemirnasinprocambarusclarkiibyhighthroughputsequencing AT yulingsun identificationandcharacterizationofsalttolerancerelativemirnasinprocambarusclarkiibyhighthroughputsequencing |