Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs
IntroductionPeriodontal disease is one of the most common oral diseases in dogs and humans. It starts with gingivitis, a reversible condition, and progresses to an irreversible condition, periodontitis. Unlike humans, the etiology of periodontal disease in dogs has not been widely studied. Many stud...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1515521/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841558744766349312 |
|---|---|
| author | Daehyun Kwon Daehyun Kwon Kisuk Bae Kwangsik Jang Kwangsik Jang Hyun Min Jo Hyun Min Jo Seong Soo Kang Seong Soo Kang Jonghoe Byun Se Eun Kim Se Eun Kim |
| author_facet | Daehyun Kwon Daehyun Kwon Kisuk Bae Kwangsik Jang Kwangsik Jang Hyun Min Jo Hyun Min Jo Seong Soo Kang Seong Soo Kang Jonghoe Byun Se Eun Kim Se Eun Kim |
| author_sort | Daehyun Kwon |
| collection | DOAJ |
| description | IntroductionPeriodontal disease is one of the most common oral diseases in dogs and humans. It starts with gingivitis, a reversible condition, and progresses to an irreversible condition, periodontitis. Unlike humans, the etiology of periodontal disease in dogs has not been widely studied. Many studies suggest that bacteria strongly implicated in human periodontal disease might also play a role in canine periodontal disease. In contrast to studies examining only the prevalence of bacteria, a recent study analyzed 336 gingival crevicular fluid (GCF) samples in dogs to evaluate the prevalence of 11 putative periodontopathic bacteria and the correlation and association of bacterial numbers individually and in combination with periodontal disease stages. Results showed that Treponema denticola (Td) was a strong prognostic biomarker for periodontitis in dogs. However, a limitation of this study was that samples were grouped according to the periodontal status of the target tooth only, without assessment of the overall oral health. Furthermore, the findings of this study revealed a need for validation in a larger sample size.Materials and methodsThis study ensured that the overall oral health assessment of dogs under 20 kg matched with sampled groups, thus eliminating the influence of environmental factors on the results. Furthermore, 1,054 GCF samples were analyzed using quantitative real-time polymerase chain reaction (qPCR) for 12 bacteria, including the same 11 putative periodontopathic bacteria [Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Td, Fusobacterium nucleatum (Fn), Prevotella nigrescens (Pn), Prevotella intermedia (Pi), Parvimonas micra (Pm), Eubacterium nodatum (En), Campylobacter rectus (Cr), and Eikenella corrodens (Ec)] and Porphyromonas gulae (P. gulae), suspected to be a major causative agent of periodontitis in dogs in some statistical evaluatioins.ResultsInterestingly, the present study found that Fn was strongly associated with gingivitis and reconfirmed a strong association between Td and periodontitis (irreversible periodontal disease). However, Aa showed no relevance, and P. gulae was not significantly associated with periodontal disease in dogs in this study.ConclusionThese findings suggest that Fn and Td would be robust biomarkers for the severity of periodontal disease in small dogs. |
| format | Article |
| id | doaj-art-106c633cbe924ba8810274d5c1c5c9d3 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-106c633cbe924ba8810274d5c1c5c9d32025-01-06T05:13:12ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15155211515521Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogsDaehyun Kwon0Daehyun Kwon1Kisuk Bae2Kwangsik Jang3Kwangsik Jang4Hyun Min Jo5Hyun Min Jo6Seong Soo Kang7Seong Soo Kang8Jonghoe Byun9Se Eun Kim10Se Eun Kim11MAY Veterinary Dental Hospital, Seoul, Republic of KoreaDepartment of Veterinary Surgery, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Republic of KoreaDepartment of Molecular Biology/Biological Sciences, School of Biomedical Sciences, Dankook University, Cheonan, Republic of KoreaDepartment of Veterinary Surgery, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Republic of KoreaBiomaterial R&BD Center, Chonnam National University, Gwangju, Republic of KoreaDepartment of Veterinary Surgery, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Republic of KoreaBiomaterial R&BD Center, Chonnam National University, Gwangju, Republic of KoreaDepartment of Veterinary Surgery, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Republic of KoreaBiomaterial R&BD Center, Chonnam National University, Gwangju, Republic of KoreaDepartment of Molecular Biology/Biological Sciences, School of Biomedical Sciences, Dankook University, Cheonan, Republic of KoreaDepartment of Veterinary Surgery, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Republic of KoreaBiomaterial R&BD Center, Chonnam National University, Gwangju, Republic of KoreaIntroductionPeriodontal disease is one of the most common oral diseases in dogs and humans. It starts with gingivitis, a reversible condition, and progresses to an irreversible condition, periodontitis. Unlike humans, the etiology of periodontal disease in dogs has not been widely studied. Many studies suggest that bacteria strongly implicated in human periodontal disease might also play a role in canine periodontal disease. In contrast to studies examining only the prevalence of bacteria, a recent study analyzed 336 gingival crevicular fluid (GCF) samples in dogs to evaluate the prevalence of 11 putative periodontopathic bacteria and the correlation and association of bacterial numbers individually and in combination with periodontal disease stages. Results showed that Treponema denticola (Td) was a strong prognostic biomarker for periodontitis in dogs. However, a limitation of this study was that samples were grouped according to the periodontal status of the target tooth only, without assessment of the overall oral health. Furthermore, the findings of this study revealed a need for validation in a larger sample size.Materials and methodsThis study ensured that the overall oral health assessment of dogs under 20 kg matched with sampled groups, thus eliminating the influence of environmental factors on the results. Furthermore, 1,054 GCF samples were analyzed using quantitative real-time polymerase chain reaction (qPCR) for 12 bacteria, including the same 11 putative periodontopathic bacteria [Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), Td, Fusobacterium nucleatum (Fn), Prevotella nigrescens (Pn), Prevotella intermedia (Pi), Parvimonas micra (Pm), Eubacterium nodatum (En), Campylobacter rectus (Cr), and Eikenella corrodens (Ec)] and Porphyromonas gulae (P. gulae), suspected to be a major causative agent of periodontitis in dogs in some statistical evaluatioins.ResultsInterestingly, the present study found that Fn was strongly associated with gingivitis and reconfirmed a strong association between Td and periodontitis (irreversible periodontal disease). However, Aa showed no relevance, and P. gulae was not significantly associated with periodontal disease in dogs in this study.ConclusionThese findings suggest that Fn and Td would be robust biomarkers for the severity of periodontal disease in small dogs.https://www.frontiersin.org/articles/10.3389/fvets.2024.1515521/fullperiodontopathic bacteriaTreponema denticolaFusobacterium nucleatumbiomarkerquantitative real-time PCRcanine gingivitis |
| spellingShingle | Daehyun Kwon Daehyun Kwon Kisuk Bae Kwangsik Jang Kwangsik Jang Hyun Min Jo Hyun Min Jo Seong Soo Kang Seong Soo Kang Jonghoe Byun Se Eun Kim Se Eun Kim Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs Frontiers in Veterinary Science periodontopathic bacteria Treponema denticola Fusobacterium nucleatum biomarker quantitative real-time PCR canine gingivitis |
| title | Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs |
| title_full | Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs |
| title_fullStr | Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs |
| title_full_unstemmed | Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs |
| title_short | Fusobacterium nucleatum and Treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs |
| title_sort | fusobacterium nucleatum and treponema denticola are robust biomarkers for gingivitis and periodontitis in small dogs |
| topic | periodontopathic bacteria Treponema denticola Fusobacterium nucleatum biomarker quantitative real-time PCR canine gingivitis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1515521/full |
| work_keys_str_mv | AT daehyunkwon fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT daehyunkwon fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT kisukbae fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT kwangsikjang fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT kwangsikjang fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT hyunminjo fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT hyunminjo fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT seongsookang fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT seongsookang fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT jonghoebyun fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT seeunkim fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs AT seeunkim fusobacteriumnucleatumandtreponemadenticolaarerobustbiomarkersforgingivitisandperiodontitisinsmalldogs |