GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC
G protein-coupled receptor 34 (GPR34) is an orphan receptor within the G protein-coupled receptor (GPCR) superfamily, and its specific role in anaplastic thyroid carcinoma (ATC) remains to be elucidated. In this study, we observed that GPR34 was aberrantly upregulated in ATC and the deletion of GPR3...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/mi/5576056 |
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| author | Bokang Yan Jiaxing Guo Meiyuan Huang Zhecheng Li Jingyue Sun Hailong Tan Weiwei Lai Shi Chang |
| author_facet | Bokang Yan Jiaxing Guo Meiyuan Huang Zhecheng Li Jingyue Sun Hailong Tan Weiwei Lai Shi Chang |
| author_sort | Bokang Yan |
| collection | DOAJ |
| description | G protein-coupled receptor 34 (GPR34) is an orphan receptor within the G protein-coupled receptor (GPCR) superfamily, and its specific role in anaplastic thyroid carcinoma (ATC) remains to be elucidated. In this study, we observed that GPR34 was aberrantly upregulated in ATC and the deletion of GPR34 inhibited tumor progression both in vivo and in vitro. Additionally, suppression of GPR34 promoted ferroptosis in ATC cells. We further identified USP8 as a deubiquitinase (DUB) for GPR34, and the effects induced by GPR34 deletion were reversible through USP8 overexpression. Moreover, targeting USP8 with the inhibitor DUB-IN-3 effectively restrained ATC growth. Together, the present study revealed the role of GPR34 in ATC progression and ferroptosis, discovered its corresponding DUBs, and proposed GPR34 as a promising target for ATC therapy. |
| format | Article |
| id | doaj-art-106aed692eba46c98e1a83d77e8bfca0 |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-106aed692eba46c98e1a83d77e8bfca02025-08-26T00:00:06ZengWileyMediators of Inflammation1466-18612025-01-01202510.1155/mi/5576056GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATCBokang Yan0Jiaxing Guo1Meiyuan Huang2Zhecheng Li3Jingyue Sun4Hailong Tan5Weiwei Lai6Shi Chang7Institute of Large-Scale Scientific Facility and Centre for Zero Magnetic Field ScienceDepartment of HematologyDepartment of PathologyDepartment of General SurgeryDepartment of PathologyDepartment of General SurgeryHunan Provincial Key Laboratory of Pediatric OrthopedicsNational Clinical Research Center for Geriatric DisordersG protein-coupled receptor 34 (GPR34) is an orphan receptor within the G protein-coupled receptor (GPCR) superfamily, and its specific role in anaplastic thyroid carcinoma (ATC) remains to be elucidated. In this study, we observed that GPR34 was aberrantly upregulated in ATC and the deletion of GPR34 inhibited tumor progression both in vivo and in vitro. Additionally, suppression of GPR34 promoted ferroptosis in ATC cells. We further identified USP8 as a deubiquitinase (DUB) for GPR34, and the effects induced by GPR34 deletion were reversible through USP8 overexpression. Moreover, targeting USP8 with the inhibitor DUB-IN-3 effectively restrained ATC growth. Together, the present study revealed the role of GPR34 in ATC progression and ferroptosis, discovered its corresponding DUBs, and proposed GPR34 as a promising target for ATC therapy.http://dx.doi.org/10.1155/mi/5576056 |
| spellingShingle | Bokang Yan Jiaxing Guo Meiyuan Huang Zhecheng Li Jingyue Sun Hailong Tan Weiwei Lai Shi Chang GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC Mediators of Inflammation |
| title | GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC |
| title_full | GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC |
| title_fullStr | GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC |
| title_full_unstemmed | GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC |
| title_short | GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC |
| title_sort | gpr34 stabilized by deubiquitinase usp8 suppresses ferroptosis of atc |
| url | http://dx.doi.org/10.1155/mi/5576056 |
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