Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosis
Introduction: Shigella continues to cause significant morbidity and mortality each year, mostly in under-five children living in developing countries. We investigated the association between Shigella virulence genes and shigellosis. Methodology: We randomly selected 61 S. flexneri strains isolat...
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The Journal of Infection in Developing Countries
2022-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/16783 |
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| author | Visnu Pritom Chowdhury Ishrat Jahan Azmi Md Ahshanul Haque Mohammad Rafiqul Islam Mahmuda Akter Shahin Mahmud Abu Syed Golam Faruque Kaisar Ali Talukder |
| author_facet | Visnu Pritom Chowdhury Ishrat Jahan Azmi Md Ahshanul Haque Mohammad Rafiqul Islam Mahmuda Akter Shahin Mahmud Abu Syed Golam Faruque Kaisar Ali Talukder |
| author_sort | Visnu Pritom Chowdhury |
| collection | DOAJ |
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Introduction: Shigella continues to cause significant morbidity and mortality each year, mostly in under-five children living in developing countries. We investigated the association between Shigella virulence genes and shigellosis.
Methodology: We randomly selected 61 S. flexneri strains isolated from patients in Bangladesh between 2009 and 2013, and evaluated the presence of 140 MDa large-virulence-plasmid (p140), and 22 virulence genes including ipaH, ial, toxin, and T3SS-related genes.
Results: We found p140 in 79% (n = 48) and ipaBCD in 90% (n = 55) strains, while seven strains were missing the p140. The prevalence of ial was 89%, ipgC and ipgE was 85%, and the prevalence for the remaining genes was < 85%. During the multivariate analysis, we found that instead of sen, the Shigella enterotoxin gene set along with several other virulence genes such as ipgA, icsB, ipgB1, spa15, and mxiC, were significantly influencing multiple clinical features relevant to shigellosis, including bloody stool, mucoid stool, and rectal straining.
Conclusions: We believe our model will help to determine the actual disease burden by directly looking for the genetic material in clinically suggestive patients, especially when detecting the causative organisms by traditional means is difficult.
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| format | Article |
| id | doaj-art-1065321f76ae4a9182fec0ca3d8fa066 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-1065321f76ae4a9182fec0ca3d8fa0662025-08-20T02:16:14ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802022-12-01161210.3855/jidc.16783Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosisVisnu Pritom Chowdhury0Ishrat Jahan Azmi1Md Ahshanul Haque2Mohammad Rafiqul Islam3Mahmuda Akter4Shahin Mahmud5Abu Syed Golam Faruque6Kaisar Ali Talukder7Department of Mathematics and Natural Sciences, BRAC University, Dhaka, BangladeshLaboratory Sciences and Services Division, International Centre for Diarrhoeal Disease Research (icddr,b), Dhaka, BangladeshNutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research (icddr,b), Dhaka, BangladeshDepartment of Mathematics and Natural Sciences, BRAC University, Dhaka, BangladeshLaboratory Sciences and Services Division, International Centre for Diarrhoeal Disease Research (icddr,b), Dhaka, BangladeshLaboratory Sciences and Services Division, International Centre for Diarrhoeal Disease Research (icddr,b), Dhaka, BangladeshNutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research (icddr,b), Dhaka, BangladeshLaboratory Sciences and Services Division, International Centre for Diarrhoeal Disease Research (icddr,b), Dhaka, Bangladesh Introduction: Shigella continues to cause significant morbidity and mortality each year, mostly in under-five children living in developing countries. We investigated the association between Shigella virulence genes and shigellosis. Methodology: We randomly selected 61 S. flexneri strains isolated from patients in Bangladesh between 2009 and 2013, and evaluated the presence of 140 MDa large-virulence-plasmid (p140), and 22 virulence genes including ipaH, ial, toxin, and T3SS-related genes. Results: We found p140 in 79% (n = 48) and ipaBCD in 90% (n = 55) strains, while seven strains were missing the p140. The prevalence of ial was 89%, ipgC and ipgE was 85%, and the prevalence for the remaining genes was < 85%. During the multivariate analysis, we found that instead of sen, the Shigella enterotoxin gene set along with several other virulence genes such as ipgA, icsB, ipgB1, spa15, and mxiC, were significantly influencing multiple clinical features relevant to shigellosis, including bloody stool, mucoid stool, and rectal straining. Conclusions: We believe our model will help to determine the actual disease burden by directly looking for the genetic material in clinically suggestive patients, especially when detecting the causative organisms by traditional means is difficult. https://jidc.org/index.php/journal/article/view/16783ShigellaShigellosisT3SSVirulence |
| spellingShingle | Visnu Pritom Chowdhury Ishrat Jahan Azmi Md Ahshanul Haque Mohammad Rafiqul Islam Mahmuda Akter Shahin Mahmud Abu Syed Golam Faruque Kaisar Ali Talukder Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosis Journal of Infection in Developing Countries Shigella Shigellosis T3SS Virulence |
| title | Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosis |
| title_full | Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosis |
| title_fullStr | Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosis |
| title_full_unstemmed | Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosis |
| title_short | Evaluation of the impact of Shigella virulence genes on the basis of clinical features observed in patients with shigellosis |
| title_sort | evaluation of the impact of shigella virulence genes on the basis of clinical features observed in patients with shigellosis |
| topic | Shigella Shigellosis T3SS Virulence |
| url | https://jidc.org/index.php/journal/article/view/16783 |
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