The influence of low-intensity ultrasound on the properties of cell cultures

In the present paper bovine kidney cell cultures were used as an experimental model for the study of the biophysical mechanism of ultrasonic action. In the first series of experiments functional and morphological changes in the cells immediately after sonication were evaluated. A decrease in viabil...

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Main Authors: Jiří ADLER, Ivo HRAZDIRA, Jitka HANZLOVÁ, Vera ZAJÍCOVÁ
Format: Article
Language:English
Published: Institute of Fundamental Technological Research Polish Academy of Sciences 2015-07-01
Series:Archives of Acoustics
Online Access:https://acoustics.ippt.pan.pl/index.php/aa/article/view/3183
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author Jiří ADLER
Ivo HRAZDIRA
Jitka HANZLOVÁ
Vera ZAJÍCOVÁ
author_facet Jiří ADLER
Ivo HRAZDIRA
Jitka HANZLOVÁ
Vera ZAJÍCOVÁ
author_sort Jiří ADLER
collection DOAJ
description In the present paper bovine kidney cell cultures were used as an experimental model for the study of the biophysical mechanism of ultrasonic action. In the first series of experiments functional and morphological changes in the cells immediately after sonication were evaluated. A decrease in viability and degenerative morphological changes in the cells were found. In the second series the sonicated cells were seeded in Roux bottles and grown in the optimal conditions. The growth properties of the cells were evaluated at different time intervals after sonication. Significant stimulation of the cell growth was demonstrated after the action of ultrasound intensity of 1.0 kWm^{-2}. However, after the action of ultrasound intensities above 3.0 kWm^{-2} the inhibition of the cell growth was found.
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id doaj-art-105d4ca3ce4344acb9865c01023ea463
institution Kabale University
issn 0137-5075
2300-262X
language English
publishDate 2015-07-01
publisher Institute of Fundamental Technological Research Polish Academy of Sciences
record_format Article
series Archives of Acoustics
spelling doaj-art-105d4ca3ce4344acb9865c01023ea4632025-08-20T03:27:56ZengInstitute of Fundamental Technological Research Polish Academy of SciencesArchives of Acoustics0137-50752300-262X2015-07-0191-2The influence of low-intensity ultrasound on the properties of cell culturesJiří ADLER0Ivo HRAZDIRA1Jitka HANZLOVÁ2Vera ZAJÍCOVÁ3Department of Biophysics, Department of Anatomy and Department of Epidemiology, Faculty of Medicine, Purkyné UniversityDepartment of Biophysics, Department of Anatomy and Department of Epidemiology, Faculty of Medicine, Purkyné UniversityDepartment of Biophysics, Department of Anatomy and Department of Epidemiology, Faculty of Medicine, Purkyné UniversityDepartment of Biophysics, Department of Anatomy and Department of Epidemiology, Faculty of Medicine, Purkyné UniversityIn the present paper bovine kidney cell cultures were used as an experimental model for the study of the biophysical mechanism of ultrasonic action. In the first series of experiments functional and morphological changes in the cells immediately after sonication were evaluated. A decrease in viability and degenerative morphological changes in the cells were found. In the second series the sonicated cells were seeded in Roux bottles and grown in the optimal conditions. The growth properties of the cells were evaluated at different time intervals after sonication. Significant stimulation of the cell growth was demonstrated after the action of ultrasound intensity of 1.0 kWm^{-2}. However, after the action of ultrasound intensities above 3.0 kWm^{-2} the inhibition of the cell growth was found.https://acoustics.ippt.pan.pl/index.php/aa/article/view/3183
spellingShingle Jiří ADLER
Ivo HRAZDIRA
Jitka HANZLOVÁ
Vera ZAJÍCOVÁ
The influence of low-intensity ultrasound on the properties of cell cultures
Archives of Acoustics
title The influence of low-intensity ultrasound on the properties of cell cultures
title_full The influence of low-intensity ultrasound on the properties of cell cultures
title_fullStr The influence of low-intensity ultrasound on the properties of cell cultures
title_full_unstemmed The influence of low-intensity ultrasound on the properties of cell cultures
title_short The influence of low-intensity ultrasound on the properties of cell cultures
title_sort influence of low intensity ultrasound on the properties of cell cultures
url https://acoustics.ippt.pan.pl/index.php/aa/article/view/3183
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