Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells
Vagus nerve stimulation through alpha7 nicotine acetylcholine receptors (α7-nAChR) signaling had been demonstrated attenuation of inflammation. This study aimed to determine whether PNU-282987, a selective α7-nAChR agonist, affected activities of matrix metalloproteinase (MMP) and inflammatory cytok...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/2401027 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558365868294144 |
|---|---|
| author | Liping Liu Hongxian Wu Qunan Cao Zhenzhen Guo Anmin Ren Qiuyan Dai |
| author_facet | Liping Liu Hongxian Wu Qunan Cao Zhenzhen Guo Anmin Ren Qiuyan Dai |
| author_sort | Liping Liu |
| collection | DOAJ |
| description | Vagus nerve stimulation through alpha7 nicotine acetylcholine receptors (α7-nAChR) signaling had been demonstrated attenuation of inflammation. This study aimed to determine whether PNU-282987, a selective α7-nAChR agonist, affected activities of matrix metalloproteinase (MMP) and inflammatory cytokines in nicotine-treatment RAW264.7 and MOVAS cells and to assess the underlying molecular mechanisms. RAW264.7 and MOVAS cells were treated with nicotine at different concentrations (0, 1, 10, and 100 ng/ml) for 0–120 min. Nicotine markedly stimulated the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and c-Jun in RAW264.7 cells. Pretreatment with U0126 significantly suppressed phosphorylation of ERK1/2 and further attenuated nicotine-induced activation of c-Jun and upregulation of MMP-2, MMP-9, monocyte chemotactic protein- (MCP-) 1, and regulated upon activation normal T cell expressed and secreted (RANTES). Similarly, nicotine treatment also increased phosphorylation of c-Jun and expressions of MMP-2, MMP-9, MCP-1, and RANTES in MOVAS cells. When cells were pretreated with PNU-282987, nicotine-induced activations of ERK1/2 and c-Jun in RAW264.7 cells and c-Jun in MOVAS cells were effectively inhibited. Furthermore, nicotine-induced secretions of MMP-2, MMP-9, MCP-1, and RANTES were remarkably downregulated. Treatment with α7-nAChR agonist inhibits nicotine-induced upregulation of MMP and inflammatory cytokines through modulating ERK1/2/AP-1 signaling in RAW264.7 cells and AP-1 in MOVAS cells, providing a new therapeutic for abdominal aortic aneurysm. |
| format | Article |
| id | doaj-art-104daa5be7c64da9b6aceb26e290cfa3 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-104daa5be7c64da9b6aceb26e290cfa32025-02-03T01:32:30ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/24010272401027Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS CellsLiping Liu0Hongxian Wu1Qunan Cao2Zhenzhen Guo3Anmin Ren4Qiuyan Dai5Department of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai Jiaotong University, Shanghai, ChinaDepartment of Cardiology, Shanghai General Hospital of Nanjing Medical University, Shanghai Jiaotong University, Shanghai, ChinaVagus nerve stimulation through alpha7 nicotine acetylcholine receptors (α7-nAChR) signaling had been demonstrated attenuation of inflammation. This study aimed to determine whether PNU-282987, a selective α7-nAChR agonist, affected activities of matrix metalloproteinase (MMP) and inflammatory cytokines in nicotine-treatment RAW264.7 and MOVAS cells and to assess the underlying molecular mechanisms. RAW264.7 and MOVAS cells were treated with nicotine at different concentrations (0, 1, 10, and 100 ng/ml) for 0–120 min. Nicotine markedly stimulated the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and c-Jun in RAW264.7 cells. Pretreatment with U0126 significantly suppressed phosphorylation of ERK1/2 and further attenuated nicotine-induced activation of c-Jun and upregulation of MMP-2, MMP-9, monocyte chemotactic protein- (MCP-) 1, and regulated upon activation normal T cell expressed and secreted (RANTES). Similarly, nicotine treatment also increased phosphorylation of c-Jun and expressions of MMP-2, MMP-9, MCP-1, and RANTES in MOVAS cells. When cells were pretreated with PNU-282987, nicotine-induced activations of ERK1/2 and c-Jun in RAW264.7 cells and c-Jun in MOVAS cells were effectively inhibited. Furthermore, nicotine-induced secretions of MMP-2, MMP-9, MCP-1, and RANTES were remarkably downregulated. Treatment with α7-nAChR agonist inhibits nicotine-induced upregulation of MMP and inflammatory cytokines through modulating ERK1/2/AP-1 signaling in RAW264.7 cells and AP-1 in MOVAS cells, providing a new therapeutic for abdominal aortic aneurysm.http://dx.doi.org/10.1155/2017/2401027 |
| spellingShingle | Liping Liu Hongxian Wu Qunan Cao Zhenzhen Guo Anmin Ren Qiuyan Dai Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells Mediators of Inflammation |
| title | Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells |
| title_full | Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells |
| title_fullStr | Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells |
| title_full_unstemmed | Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells |
| title_short | Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264.7 and MOVAS Cells |
| title_sort | stimulation of alpha7 nicotinic acetylcholine receptor attenuates nicotine induced upregulation of mmp mcp 1 and rantes through modulating erk1 2 ap 1 signaling pathway in raw264 7 and movas cells |
| url | http://dx.doi.org/10.1155/2017/2401027 |
| work_keys_str_mv | AT lipingliu stimulationofalpha7nicotinicacetylcholinereceptorattenuatesnicotineinducedupregulationofmmpmcp1andrantesthroughmodulatingerk12ap1signalingpathwayinraw2647andmovascells AT hongxianwu stimulationofalpha7nicotinicacetylcholinereceptorattenuatesnicotineinducedupregulationofmmpmcp1andrantesthroughmodulatingerk12ap1signalingpathwayinraw2647andmovascells AT qunancao stimulationofalpha7nicotinicacetylcholinereceptorattenuatesnicotineinducedupregulationofmmpmcp1andrantesthroughmodulatingerk12ap1signalingpathwayinraw2647andmovascells AT zhenzhenguo stimulationofalpha7nicotinicacetylcholinereceptorattenuatesnicotineinducedupregulationofmmpmcp1andrantesthroughmodulatingerk12ap1signalingpathwayinraw2647andmovascells AT anminren stimulationofalpha7nicotinicacetylcholinereceptorattenuatesnicotineinducedupregulationofmmpmcp1andrantesthroughmodulatingerk12ap1signalingpathwayinraw2647andmovascells AT qiuyandai stimulationofalpha7nicotinicacetylcholinereceptorattenuatesnicotineinducedupregulationofmmpmcp1andrantesthroughmodulatingerk12ap1signalingpathwayinraw2647andmovascells |