Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings.
COVID-19 resulted in extensive morbidity and mortality worldwide. SARS-CoV-2 evolved rapidly, with increasing transmission due to Variants of Concern (VOC). Identifying VOC became important but genome submissions from low-middle income countries (LMIC) remained low leading to gaps in genomic epidemi...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLOS Global Public Health |
| Online Access: | https://doi.org/10.1371/journal.pgph.0001896 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850159949827538944 |
|---|---|
| author | Asghar Nasir Uzma Bashir Aamir Akbar Kanji Ali Raza Bukhari Zeeshan Ansar Najia Karim Ghanchi Kiran Iqbal Masood Azra Samreen Nazneen Islam Samina Ghani M Asif Syed Mansoor Wassan Syed Faisal Mahmood Zahra Hasan |
| author_facet | Asghar Nasir Uzma Bashir Aamir Akbar Kanji Ali Raza Bukhari Zeeshan Ansar Najia Karim Ghanchi Kiran Iqbal Masood Azra Samreen Nazneen Islam Samina Ghani M Asif Syed Mansoor Wassan Syed Faisal Mahmood Zahra Hasan |
| author_sort | Asghar Nasir |
| collection | DOAJ |
| description | COVID-19 resulted in extensive morbidity and mortality worldwide. SARS-CoV-2 evolved rapidly, with increasing transmission due to Variants of Concern (VOC). Identifying VOC became important but genome submissions from low-middle income countries (LMIC) remained low leading to gaps in genomic epidemiology. We demonstrate the use of a specific mutation RT-PCR based approach to identify VOC in SARS-CoV-2 positive samples through the pandemic in Pakistan. We selected 2150 SARS-CoV-2 PCR positive respiratory specimens tested between April 2021 and February 2022, at the Aga Khan University Hospital Clinical Laboratories, Karachi, Pakistan. Commercially available RT-PCR assays were used as required for mutations in Spike protein (N501Y, A570D, E484K, K417N, L452R, P681R and deletion69_70) to identify Alpha, Beta, Gamma, Delta, and Omicron variants respectively. Three pandemic waves associated with Alpha, Delta and Omicron occurred during the study period. Of the samples screened, VOC were identified in 81.7% of cases comprising mainly; Delta (37.2%), Alpha (29.8%) and Omicron (17.1%) variants. During 2021, Alpha variants were predominant in April and May; Beta and Gamma variants emerged in May and peaked in June; the Delta variant peaked in July and remained predominant until November. Omicron (BA.1) emerged in December 2021 and remained predominant until February 2022. The CT values of Alpha, Beta, Gamma and Delta were all significantly higher than that of Omicron variants (p<0.0001). We observed VOC through the pandemic waves using spike mutation specific RT-PCR assays. We show the spike mutation specific RT-PCR assay is a rapid, low-cost and adaptable for the identification of VOC as an adjunct approach to NGS to effectively inform the public health response. Further, by associating the VOC with CT values of its diagnostic PCR we gain information regarding the viral load of samples and therefore the level of transmission and disease severity in the population. |
| format | Article |
| id | doaj-art-103696218eee404f9190e4377c1be86d |
| institution | OA Journals |
| issn | 2767-3375 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLOS Global Public Health |
| spelling | doaj-art-103696218eee404f9190e4377c1be86d2025-08-20T02:23:19ZengPublic Library of Science (PLoS)PLOS Global Public Health2767-33752023-01-0136e000189610.1371/journal.pgph.0001896Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings.Asghar NasirUzma Bashir AamirAkbar KanjiAli Raza BukhariZeeshan AnsarNajia Karim GhanchiKiran Iqbal MasoodAzra SamreenNazneen IslamSamina GhaniM Asif SyedMansoor WassanSyed Faisal MahmoodZahra HasanCOVID-19 resulted in extensive morbidity and mortality worldwide. SARS-CoV-2 evolved rapidly, with increasing transmission due to Variants of Concern (VOC). Identifying VOC became important but genome submissions from low-middle income countries (LMIC) remained low leading to gaps in genomic epidemiology. We demonstrate the use of a specific mutation RT-PCR based approach to identify VOC in SARS-CoV-2 positive samples through the pandemic in Pakistan. We selected 2150 SARS-CoV-2 PCR positive respiratory specimens tested between April 2021 and February 2022, at the Aga Khan University Hospital Clinical Laboratories, Karachi, Pakistan. Commercially available RT-PCR assays were used as required for mutations in Spike protein (N501Y, A570D, E484K, K417N, L452R, P681R and deletion69_70) to identify Alpha, Beta, Gamma, Delta, and Omicron variants respectively. Three pandemic waves associated with Alpha, Delta and Omicron occurred during the study period. Of the samples screened, VOC were identified in 81.7% of cases comprising mainly; Delta (37.2%), Alpha (29.8%) and Omicron (17.1%) variants. During 2021, Alpha variants were predominant in April and May; Beta and Gamma variants emerged in May and peaked in June; the Delta variant peaked in July and remained predominant until November. Omicron (BA.1) emerged in December 2021 and remained predominant until February 2022. The CT values of Alpha, Beta, Gamma and Delta were all significantly higher than that of Omicron variants (p<0.0001). We observed VOC through the pandemic waves using spike mutation specific RT-PCR assays. We show the spike mutation specific RT-PCR assay is a rapid, low-cost and adaptable for the identification of VOC as an adjunct approach to NGS to effectively inform the public health response. Further, by associating the VOC with CT values of its diagnostic PCR we gain information regarding the viral load of samples and therefore the level of transmission and disease severity in the population.https://doi.org/10.1371/journal.pgph.0001896 |
| spellingShingle | Asghar Nasir Uzma Bashir Aamir Akbar Kanji Ali Raza Bukhari Zeeshan Ansar Najia Karim Ghanchi Kiran Iqbal Masood Azra Samreen Nazneen Islam Samina Ghani M Asif Syed Mansoor Wassan Syed Faisal Mahmood Zahra Hasan Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings. PLOS Global Public Health |
| title | Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings. |
| title_full | Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings. |
| title_fullStr | Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings. |
| title_full_unstemmed | Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings. |
| title_short | Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings. |
| title_sort | tracking sars cov 2 variants through pandemic waves using rt pcr testing in low resource settings |
| url | https://doi.org/10.1371/journal.pgph.0001896 |
| work_keys_str_mv | AT asgharnasir trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT uzmabashiraamir trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT akbarkanji trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT alirazabukhari trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT zeeshanansar trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT najiakarimghanchi trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT kiraniqbalmasood trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT azrasamreen trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT nazneenislam trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT saminaghani trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT masifsyed trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT mansoorwassan trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT syedfaisalmahmood trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings AT zahrahasan trackingsarscov2variantsthroughpandemicwavesusingrtpcrtestinginlowresourcesettings |