Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil

Isolation and genetic characterization of Toxoplasma gondii from chickens from public markets in Pernambuco State, Brazil

This study aimed to evaluate the presence and viability of Toxoplasma gondii in chickens intended for human consumption in the Pernambuco State, Brazil. Blood and tissue samples were collected from 25 chickens sold in markets in Recife, Pernambuco. Samples were evaluated by indirect immunofluorescen...

Full description

Saved in:
Bibliographic Details
Main Authors: Renato Amorim da Silva, Raissa Santana Renovato, Hannah Tsuruzaki Kirzner de Barros e Silva, Maria Luiza Didier Marques, Pollyanne Raysa Fernandes Oliveira, Jéssica de Crasto Souza Carvalho-Reis, Paul M. Bartley, Frank Katzer, Érika Fernanda Torres Samico-Fernandes, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Current Research in Parasitology and Vector-Borne Diseases
Subjects:
Toxoplasma gondii
Chicken
Mouse bioassay
PCR-RFLP
Public health
Zoonosis
Online Access:http://www.sciencedirect.com/science/article/pii/S2667114X24000384
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to evaluate the presence and viability of Toxoplasma gondii in chickens intended for human consumption in the Pernambuco State, Brazil. Blood and tissue samples were collected from 25 chickens sold in markets in Recife, Pernambuco. Samples were evaluated by indirect immunofluorescence assay (IFA) to detect antibodies to T. gondii. Pools of brain and heart of seropositive chickens were subjected to bioassay in two Swiss Webster mice, which were evaluated for 45 days then tested by IFA to detect seroconversion. The mice were euthanized, and their brains were evaluated for cysts. Peritoneal lavage was also conducted in mice that exhibited clinical signs. Brains containing cysts or peritoneal lavage with tachyzoites were inoculated into MA-104 cells. Brains of mice inoculated with the same tissue were pooled and analysed by ITS1-PCR. We obtained a frequency of antibodies to T. gondii of 68.00% (17/25) in chickens, and a seroconversion rate of 70.58% (24/34) in mice. Detection of Toxoplasma ITS1 DNA confirmed an isolation rate of 41.1% (7/17). Three isolates were characterized by mnPCR-RFLP as genotypes ToxoDB#36 and ToxoDB#114. We highlight the occurrence of ToxoDB#36 in chickens in Pernambuco State and the parasitesʼ viability in chickens intended for human consumption.
ISSN:2667-114X

Similar Items