Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity

The eco-friendly and cost-effective biological synthesis of nanomaterials is rapidly gaining attention. This study synthesized silver nanoparticles (AgNPs) using an aqueous extract of Senna italica leaves and silver nitrate (AgNO3). The synthesized AgNPs were characterized using UV-Vis spectroscopy,...

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Main Authors: Emad Abada, Fatimah Habib, Abdullah Mashraqi, Yosra Modafer, Wail Alsolami, Khatib Ismail, Abdullah Ali Alamri, Abadi M. Mashlawi, Abdel-Rahman M. Shater
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/ijm/2072594
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author Emad Abada
Fatimah Habib
Abdullah Mashraqi
Yosra Modafer
Wail Alsolami
Khatib Ismail
Abdullah Ali Alamri
Abadi M. Mashlawi
Abdel-Rahman M. Shater
author_facet Emad Abada
Fatimah Habib
Abdullah Mashraqi
Yosra Modafer
Wail Alsolami
Khatib Ismail
Abdullah Ali Alamri
Abadi M. Mashlawi
Abdel-Rahman M. Shater
author_sort Emad Abada
collection DOAJ
description The eco-friendly and cost-effective biological synthesis of nanomaterials is rapidly gaining attention. This study synthesized silver nanoparticles (AgNPs) using an aqueous extract of Senna italica leaves and silver nitrate (AgNO3). The synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). UV-Vis spectroscopy confirmed the formation of AgNPs, displaying a characteristic surface plasmon resonance peak at 445 nm. TEM and SEM analyses revealed spherical nanoparticles with sizes ranging from 12.7 to 24 nm. FTIR spectra identified bands at 1636 and 3496 cm−1, corresponding to C=O and O-H groups, indicating their role in stabilizing the nanoparticles. XRD analysis revealed diffraction planes at 111, 200, 220, and 311, consistent with the face-centered cubic structure of silver. The AgNPs demonstrated significant antimicrobial activity against fungi and Gram-negative and Gram-positive bacteria, with Escherichia coli showing the highest sensitivity (MIC=0.014  μg/mL). SEM analysis of E. coli showed that untreated cells retained their normal morphology, whereas AgNP-treated cells appeared shriveled and deformed. These results underscore the potential of Senna italica–derived AgNPs as effective antimicrobial agents. Future studies will be aimed at investigating the detailed mechanisms underlying the effects of AgNPs on bacterial cell structure and growth.
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spelling doaj-art-1025ac147a2e457ca0157cfd6076db372025-08-20T01:50:57ZengWileyInternational Journal of Microbiology1687-91982025-01-01202510.1155/ijm/2072594Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial ActivityEmad Abada0Fatimah Habib1Abdullah Mashraqi2Yosra Modafer3Wail Alsolami4Khatib Ismail5Abdullah Ali Alamri6Abadi M. Mashlawi7Abdel-Rahman M. Shater8Department of BiologyDepartment of BiologyDepartment of BiologyDepartment of BiologyDepartment of BiologyDepartment of BiologyDepartment of Physical SciencesDepartment of BiologyDepartment of BiologyThe eco-friendly and cost-effective biological synthesis of nanomaterials is rapidly gaining attention. This study synthesized silver nanoparticles (AgNPs) using an aqueous extract of Senna italica leaves and silver nitrate (AgNO3). The synthesized AgNPs were characterized using UV-Vis spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and X-ray diffraction (XRD). UV-Vis spectroscopy confirmed the formation of AgNPs, displaying a characteristic surface plasmon resonance peak at 445 nm. TEM and SEM analyses revealed spherical nanoparticles with sizes ranging from 12.7 to 24 nm. FTIR spectra identified bands at 1636 and 3496 cm−1, corresponding to C=O and O-H groups, indicating their role in stabilizing the nanoparticles. XRD analysis revealed diffraction planes at 111, 200, 220, and 311, consistent with the face-centered cubic structure of silver. The AgNPs demonstrated significant antimicrobial activity against fungi and Gram-negative and Gram-positive bacteria, with Escherichia coli showing the highest sensitivity (MIC=0.014  μg/mL). SEM analysis of E. coli showed that untreated cells retained their normal morphology, whereas AgNP-treated cells appeared shriveled and deformed. These results underscore the potential of Senna italica–derived AgNPs as effective antimicrobial agents. Future studies will be aimed at investigating the detailed mechanisms underlying the effects of AgNPs on bacterial cell structure and growth.http://dx.doi.org/10.1155/ijm/2072594
spellingShingle Emad Abada
Fatimah Habib
Abdullah Mashraqi
Yosra Modafer
Wail Alsolami
Khatib Ismail
Abdullah Ali Alamri
Abadi M. Mashlawi
Abdel-Rahman M. Shater
Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity
International Journal of Microbiology
title Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity
title_full Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity
title_fullStr Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity
title_full_unstemmed Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity
title_short Eco-Friendly Synthesis and Characterization of Senna italica–Derived Silver Nanoparticles With Broad-Spectrum Antimicrobial Activity
title_sort eco friendly synthesis and characterization of senna italica derived silver nanoparticles with broad spectrum antimicrobial activity
url http://dx.doi.org/10.1155/ijm/2072594
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