White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTI

Abstract Thalassemia is a hereditary hemoglobinopathy characterized by severe anemia and iron overload, which may affect brain microstructure. This study aims to investigate changes in the white matter (WM) microstructure of patients with β-thalassemia using diffusion tensor imaging (DTI) and tract-...

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Main Authors: Mingrui Yang, Guowei Chen, Chaotian Luo, Cheng Tang, Fei Peng, Rong Kong, Peng Peng
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-99505-8
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author Mingrui Yang
Guowei Chen
Chaotian Luo
Cheng Tang
Fei Peng
Rong Kong
Peng Peng
author_facet Mingrui Yang
Guowei Chen
Chaotian Luo
Cheng Tang
Fei Peng
Rong Kong
Peng Peng
author_sort Mingrui Yang
collection DOAJ
description Abstract Thalassemia is a hereditary hemoglobinopathy characterized by severe anemia and iron overload, which may affect brain microstructure. This study aims to investigate changes in the white matter (WM) microstructure of patients with β-thalassemia using diffusion tensor imaging (DTI) and tract-based spatial statistics (TBSS) analysis. The study included 40 patients with β-thalassemia (aged 18–45 years, mean age 30.65 years) and 40 healthy controls (aged 18–45 years, mean age 30.13 years). All participants underwent 3.0 T MRI scans. DTI data were processed using the FSL software package, focusing on the assessment of four indices: fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD). The receiver operating characteristic (ROC) curve area under the curve (AUC) was calculated for these indices to highlight the potential value of white matter regions in distinguishing between patient and control group. Compared to the control group, patients with β-thalassemia showed decreased FA and increased MD and RD in multiple white matter tracts. ROC curve analysis showed high AUC values for FA, MD, and RD (0.868, 0.854, and 0.847, respectively). The results suggest that patients with β-thalassemia may have widespread abnormalities in the white matter microstructure.
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spelling doaj-art-10191e0dacad418abe779ed650ff9e6d2025-08-20T01:53:23ZengNature PortfolioScientific Reports2045-23222025-05-0115111010.1038/s41598-025-99505-8White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTIMingrui Yang0Guowei Chen1Chaotian Luo2Cheng Tang3Fei Peng4Rong Kong5Peng Peng6Department of Radiology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Radiology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Radiology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Radiology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Radiology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Radiology, The First Affiliated Hospital of Guangxi Medical UniversityDepartment of Radiology, The First Affiliated Hospital of Guangxi Medical UniversityAbstract Thalassemia is a hereditary hemoglobinopathy characterized by severe anemia and iron overload, which may affect brain microstructure. This study aims to investigate changes in the white matter (WM) microstructure of patients with β-thalassemia using diffusion tensor imaging (DTI) and tract-based spatial statistics (TBSS) analysis. The study included 40 patients with β-thalassemia (aged 18–45 years, mean age 30.65 years) and 40 healthy controls (aged 18–45 years, mean age 30.13 years). All participants underwent 3.0 T MRI scans. DTI data were processed using the FSL software package, focusing on the assessment of four indices: fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD). The receiver operating characteristic (ROC) curve area under the curve (AUC) was calculated for these indices to highlight the potential value of white matter regions in distinguishing between patient and control group. Compared to the control group, patients with β-thalassemia showed decreased FA and increased MD and RD in multiple white matter tracts. ROC curve analysis showed high AUC values for FA, MD, and RD (0.868, 0.854, and 0.847, respectively). The results suggest that patients with β-thalassemia may have widespread abnormalities in the white matter microstructure.https://doi.org/10.1038/s41598-025-99505-8ThalassemiaWhite matterDiffusion tensor imagingTract-based spatial statisticsMicrostructural abnormalitiesIron overload
spellingShingle Mingrui Yang
Guowei Chen
Chaotian Luo
Cheng Tang
Fei Peng
Rong Kong
Peng Peng
White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTI
Scientific Reports
Thalassemia
White matter
Diffusion tensor imaging
Tract-based spatial statistics
Microstructural abnormalities
Iron overload
title White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTI
title_full White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTI
title_fullStr White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTI
title_full_unstemmed White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTI
title_short White matter microstructure abnormalities in thalassemia patients using TBSS analysis based on DTI
title_sort white matter microstructure abnormalities in thalassemia patients using tbss analysis based on dti
topic Thalassemia
White matter
Diffusion tensor imaging
Tract-based spatial statistics
Microstructural abnormalities
Iron overload
url https://doi.org/10.1038/s41598-025-99505-8
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