Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides
We tested the effects of galactoglucomannan oligosaccharides (GGMOs) and/or cadmium (Cd) on peroxidase activity and the proteome in maize (Zea mays L.) roots and leaves. Our previous work confirmed that GGMOs ameliorate the symptoms of Cd stress in seedlings. Here, the plants were hydroponically cul...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000685 |
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| author | Diana Hačkuličová Eva Labancová Zuzana Vivodová Maksym Danchenko Kristína Holeková Marko Bajus Danica Kučerová Peter Baráth Karin Kollárová |
| author_facet | Diana Hačkuličová Eva Labancová Zuzana Vivodová Maksym Danchenko Kristína Holeková Marko Bajus Danica Kučerová Peter Baráth Karin Kollárová |
| author_sort | Diana Hačkuličová |
| collection | DOAJ |
| description | We tested the effects of galactoglucomannan oligosaccharides (GGMOs) and/or cadmium (Cd) on peroxidase activity and the proteome in maize (Zea mays L.) roots and leaves. Our previous work confirmed that GGMOs ameliorate the symptoms of Cd stress in seedlings. Here, the plants were hydroponically cultivated for 7 days, and the protein content and peroxidase activity were estimated in intracellular, neutral cell wall, and acidic cell wall protein fractions. The peroxidase activity varied between the plant organs as well as among the fractions and treatments. The GGMOs in the presence of Cd did not significantly influence content of peroxidases but modulated their activity, which implies posttranslational regulation. The changes in the content of various proteins (e.g., related to the defence reactions, cell wall structure/metabolism, and activation of plant hormones) caused by GGMOs and Cd indicate possible protective mechanisms that improve the vitality of maize seedlings exposed to metal stress. GGMOs partially reverted Cd-induced protein disbalance, which was a reoccurring phenomenon of mitigation in leaves. |
| format | Article |
| id | doaj-art-1016d9405db949dd92ba45c3bb1800ca |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-1016d9405db949dd92ba45c3bb1800ca2025-02-12T05:30:07ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117732Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharidesDiana Hačkuličová0Eva Labancová1Zuzana Vivodová2Maksym Danchenko3Kristína Holeková4Marko Bajus5Danica Kučerová6Peter Baráth7Karin Kollárová8Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaInstitute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaCorresponding author.; Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava 845 38, SlovakiaWe tested the effects of galactoglucomannan oligosaccharides (GGMOs) and/or cadmium (Cd) on peroxidase activity and the proteome in maize (Zea mays L.) roots and leaves. Our previous work confirmed that GGMOs ameliorate the symptoms of Cd stress in seedlings. Here, the plants were hydroponically cultivated for 7 days, and the protein content and peroxidase activity were estimated in intracellular, neutral cell wall, and acidic cell wall protein fractions. The peroxidase activity varied between the plant organs as well as among the fractions and treatments. The GGMOs in the presence of Cd did not significantly influence content of peroxidases but modulated their activity, which implies posttranslational regulation. The changes in the content of various proteins (e.g., related to the defence reactions, cell wall structure/metabolism, and activation of plant hormones) caused by GGMOs and Cd indicate possible protective mechanisms that improve the vitality of maize seedlings exposed to metal stress. GGMOs partially reverted Cd-induced protein disbalance, which was a reoccurring phenomenon of mitigation in leaves.http://www.sciencedirect.com/science/article/pii/S0147651325000685Cadmium toxicityCell wallDiscovery proteomicsPeroxidasesStress mitigationZea mays L |
| spellingShingle | Diana Hačkuličová Eva Labancová Zuzana Vivodová Maksym Danchenko Kristína Holeková Marko Bajus Danica Kučerová Peter Baráth Karin Kollárová Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides Ecotoxicology and Environmental Safety Cadmium toxicity Cell wall Discovery proteomics Peroxidases Stress mitigation Zea mays L |
| title | Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides |
| title_full | Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides |
| title_fullStr | Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides |
| title_full_unstemmed | Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides |
| title_short | Modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides |
| title_sort | modification of peroxidase activity and proteome in maize exposed to cadmium in the presence of galactoglucomannan oligosaccharides |
| topic | Cadmium toxicity Cell wall Discovery proteomics Peroxidases Stress mitigation Zea mays L |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000685 |
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