Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, India

Two fish species Acanthurus mata and Ephippus orbis of the order Acanthuriformes were collected from Gopalpur-onsea, Bay of Bengal, where they were recorded for the first time. Both the fishes were identified using the conventional taxonomic methods followed by the molecular taxonomic method of...

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Main Authors: BIJAYALAXMI SAHU, TAPAN KUMAR BARIK, AMIYA KUMAR PATEL
Format: Article
Language:English
Published: Asian Fisheries Society 2025-06-01
Series:Asian Fisheries Science
Online Access:https://asianfisheriessociety.org/publication/downloadfile.php?id=1464&file=Y0dSbUx6QXdOamN6T1Rnd01ERTNOVEV5TkRZeE5UQXVjR1Jt
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author BIJAYALAXMI SAHU
TAPAN KUMAR BARIK
AMIYA KUMAR PATEL
author_facet BIJAYALAXMI SAHU
TAPAN KUMAR BARIK
AMIYA KUMAR PATEL
author_sort BIJAYALAXMI SAHU
collection DOAJ
description Two fish species Acanthurus mata and Ephippus orbis of the order Acanthuriformes were collected from Gopalpur-onsea, Bay of Bengal, where they were recorded for the first time. Both the fishes were identified using the conventional taxonomic methods followed by the molecular taxonomic method of DNA barcoding using the mitochondrial cytochrome c oxidase subunit I (COI) gene. All COI barcodes produced in this investigation were matched with reference sequences of anticipated species by morphological identification. Based on DNA barcodes, neighbour-joining, and maximum likelihood phylogenetic trees were constructed, and all of the specimens found were clustered according to their species-level taxonomic categorisation. To derive haplotype diversity, a median-joining network was built. The current study additionally looked at the number of variable sites, parsimony informative sites, nucleotide diversity, and variation in amino acids. Significant genetic variations were detected among A. mata species from various geographic regions. However, E. orbis still has highly conserved natural populations with no significant variations. The findings of this work provide information on the phylogeny, population dynamics, divergence time, and genetic diversity of both fish species as well as substantial validation for the use of DNA barcode sequences for tracking species diversity.
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institution Kabale University
issn 0116-6514
2073-3720
language English
publishDate 2025-06-01
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series Asian Fisheries Science
spelling doaj-art-10150604cd5f43c5be6bb54e28a34da02025-08-20T03:32:19ZengAsian Fisheries SocietyAsian Fisheries Science0116-65142073-37202025-06-0138210.33997/j.afs.2025.38.2.004Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, IndiaBIJAYALAXMI SAHUTAPAN KUMAR BARIKAMIYA KUMAR PATEL Two fish species Acanthurus mata and Ephippus orbis of the order Acanthuriformes were collected from Gopalpur-onsea, Bay of Bengal, where they were recorded for the first time. Both the fishes were identified using the conventional taxonomic methods followed by the molecular taxonomic method of DNA barcoding using the mitochondrial cytochrome c oxidase subunit I (COI) gene. All COI barcodes produced in this investigation were matched with reference sequences of anticipated species by morphological identification. Based on DNA barcodes, neighbour-joining, and maximum likelihood phylogenetic trees were constructed, and all of the specimens found were clustered according to their species-level taxonomic categorisation. To derive haplotype diversity, a median-joining network was built. The current study additionally looked at the number of variable sites, parsimony informative sites, nucleotide diversity, and variation in amino acids. Significant genetic variations were detected among A. mata species from various geographic regions. However, E. orbis still has highly conserved natural populations with no significant variations. The findings of this work provide information on the phylogeny, population dynamics, divergence time, and genetic diversity of both fish species as well as substantial validation for the use of DNA barcode sequences for tracking species diversity.https://asianfisheriessociety.org/publication/downloadfile.php?id=1464&file=Y0dSbUx6QXdOamN6T1Rnd01ERTNOVEV5TkRZeE5UQXVjR1Jt
spellingShingle BIJAYALAXMI SAHU
TAPAN KUMAR BARIK
AMIYA KUMAR PATEL
Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, India
Asian Fisheries Science
title Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, India
title_full Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, India
title_fullStr Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, India
title_full_unstemmed Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, India
title_short Molecular Phylogenetic Analyses of Two Ray-Finned Fishes Acanthurus mata (Cuvier, 1829) and Ephippus orbis (Bloch, 1787) From the Odisha Coast, Bay of Bengal, India
title_sort molecular phylogenetic analyses of two ray finned fishes acanthurus mata cuvier 1829 and ephippus orbis bloch 1787 from the odisha coast bay of bengal india
url https://asianfisheriessociety.org/publication/downloadfile.php?id=1464&file=Y0dSbUx6QXdOamN6T1Rnd01ERTNOVEV5TkRZeE5UQXVjR1Jt
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AT tapankumarbarik molecularphylogeneticanalysesoftworayfinnedfishesacanthurusmatacuvier1829andephippusorbisbloch1787fromtheodishacoastbayofbengalindia
AT amiyakumarpatel molecularphylogeneticanalysesoftworayfinnedfishesacanthurusmatacuvier1829andephippusorbisbloch1787fromtheodishacoastbayofbengalindia