Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant
Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound...
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Mustansiriyah University
2020-08-01
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| Series: | Al-Mustansiriyah Journal of Science |
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| Online Access: | http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/670 |
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| author | Ali A. Taha Nahida J. Hameed Farah Hamed Ali |
| author_facet | Ali A. Taha Nahida J. Hameed Farah Hamed Ali |
| author_sort | Ali A. Taha |
| collection | DOAJ |
| description | Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation. |
| format | Article |
| id | doaj-art-10122b6d212a4dfea97dee8274a2f0b2 |
| institution | OA Journals |
| issn | 1814-635X 2521-3520 |
| language | English |
| publishDate | 2020-08-01 |
| publisher | Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Science |
| spelling | doaj-art-10122b6d212a4dfea97dee8274a2f0b22025-08-20T02:18:23ZengMustansiriyah UniversityAl-Mustansiriyah Journal of Science1814-635X2521-35202020-08-01313142010.23851/mjs.v31i3.670327Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth PlantAli A. Taha0Nahida J. Hameed1Farah Hamed Ali2Applied Science Department, Biotechnology Division, University of Technology.Applied Science Department, Materials Division, University of Technology.Applied Science Department, Materials Division, University of Technology.Polycyclic aromatic hydrocarbons (PAHs) are recognized as a toxic, mutagenic and/or carcinogenic compounds, and their pollution of soil and aquifer is of increasing environmentally risk. Laccases (E.C. 1.10.3.2) are phenoloxidases catalyze the oxidation of PAHs in the presence of a mediator compound and hyacinth plant. In this study laccase from Trametes versicolor was immobilized into chitosan, and the potential to oxidize anthracene in the presence of 1-hydroxybenzotriazole (HBT) was examined. Results indicated that the immobilization enhanced the stability of laccase against temperature, pH, inhibitors and loading time compared with the other cases. The immobilized laccase-mediator system was as efficient as the free enzyme for oxidizing the tested PAHs. After 24h. of incubation, immobilized laccase–HBT showed a system oxidization more than immobilized laccase without (HBT) of PAHs; Chitosan with hyacinth plant and (HBT) resulted better conversion than chitosan with or without HBT. These results indicate a new chance for applying the immobilized laccase in bioremediation.http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/670polycyclic aromatic hydrocarbons pahimmobilized laccasetrametes versicolorbioremediationhbtconversationhyacinth plant. |
| spellingShingle | Ali A. Taha Nahida J. Hameed Farah Hamed Ali Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant Al-Mustansiriyah Journal of Science polycyclic aromatic hydrocarbons pah immobilized laccase trametes versicolor bioremediation hbt conversation hyacinth plant. |
| title | Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant |
| title_full | Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant |
| title_fullStr | Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant |
| title_full_unstemmed | Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant |
| title_short | Degradation of Anthracene by Immobilizing Laccase From Trametes Versicolor onto Chitosan Beads and Hyacinth Plant |
| title_sort | degradation of anthracene by immobilizing laccase from trametes versicolor onto chitosan beads and hyacinth plant |
| topic | polycyclic aromatic hydrocarbons pah immobilized laccase trametes versicolor bioremediation hbt conversation hyacinth plant. |
| url | http://mjs.uomustansiriyah.edu.iq/ojs1/index.php/MJS/article/view/670 |
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