GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal Plants
Study’s Excerpt: • GC-MS profiles of some selected medicinal plants were accessed. • Beta-lactamase inhibition assay revealed some of the plants are potential inhibitors of beta-lacatamase enzymes. • Molecular docking simulations showed significant interactions between the bioactive compounds and pr...
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| Format: | Article |
| Language: | English |
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Umaru Musa Yar'adua University, Katsina, Nigeria
2024-12-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/660 |
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| author | Ubamaka W Ike Osinachi Samdavid Okpara Chidi Ijeoma Nosiri G Enyinnaya Eze Chinedu Aguwamba Chinomso Friday Aaron Okamgba, C. O. S C Okereke |
| author_facet | Ubamaka W Ike Osinachi Samdavid Okpara Chidi Ijeoma Nosiri G Enyinnaya Eze Chinedu Aguwamba Chinomso Friday Aaron Okamgba, C. O. S C Okereke |
| author_sort | Ubamaka W Ike |
| collection | DOAJ |
| description | Study’s Excerpt:
• GC-MS profiles of some selected medicinal plants were accessed.
• Beta-lactamase inhibition assay revealed some of the plants are potential inhibitors of beta-lacatamase enzymes.
• Molecular docking simulations showed significant interactions between the bioactive compounds and protein receptors.
Full Abstract:
Bacterial infections are continually developing resistance to conventional antibiotic agents, thereby prompting the search for bioactive compounds from plant parts that would serve as lead molecules in the discovery and development of new drugs. There is a need to explore sustainable, innovative, and safe natural therapeutic methods in preventing and managing AMR infections. Beta-lactamase secretion by bacteria is one of the main resistant mechanisms bacterial enzymes use to resist antibiotics. Hence, this study investigated the beta-lactamase inhibition potential of Salacia nitida and Rauvolfia vomitoria root extracts. Gas chromatography-mass spectrometry (GC-MS) analysis was carried out on the root extracts of S. nitida and R. vomitoria. Molecular docking was performed to determine the binding affinity and energy between class A beta-lactamase and selected bioactive compounds. Results from the beta-lactamase inhibition assay showed that S. nitida and R. vomitoria root extracts had 65.87% and 69.89% inhibitory activity, respectively, against the beta-lactamase enzyme, suggesting that these plant extracts have the potential to be used as a beta-lactamase inhibitor in combination with beta-lactam antibiotics. The GC-MS results revealed 10 bioactive compounds in S. nitida and 28 compounds in R. vomitoria. Molecular docking results showed favourable hydrogen bonds and Van der waal interactions between beta-lactamase and selected bioactive compounds. The findings suggest that these plant extracts possess significant beta-lactamase inhibition activity and this can further lend pharmacological support to their folklore uses as antibiotic agents.
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| format | Article |
| id | doaj-art-1011e10bfeb24c30b69a63440c3e5436 |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-1011e10bfeb24c30b69a63440c3e54362025-08-20T03:46:04ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222024-12-019210.47430/ujmr.2492.030GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal PlantsUbamaka W Ike0Osinachi Samdavid Okpara1Chidi Ijeoma Nosiri2G Enyinnaya Eze3Chinedu Aguwamba4Chinomso Friday Aaron5Okamgba, C. O.6S C Okereke7Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences Abia State University, Uturu, NigeriaDepartment of Biochemistry, Faculty of Biological Sciences, Abia State University, Uturu, NigeriaDepartment of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences Abia State University, Uturu, NigeriaDepartment of Biochemistry, Faculty of Biological Sciences, Abia State University, Uturu, NigeriaDepartment of Biochemistry, Faculty of Biological Sciences, Clifford University, Isialangwa, NigeriaDepartment of Biochemistry, Faculty of Biological Sciences, Abia State University, Uturu, NigeriaDepartment of Microbiology and Biotechnology, Faculty of Medical Laboratory Sciences, Abia State University, Uturu, NigeriaDepartment of Biochemistry, Faculty of Biological Sciences, Abia State University, Uturu, NigeriaStudy’s Excerpt: • GC-MS profiles of some selected medicinal plants were accessed. • Beta-lactamase inhibition assay revealed some of the plants are potential inhibitors of beta-lacatamase enzymes. • Molecular docking simulations showed significant interactions between the bioactive compounds and protein receptors. Full Abstract: Bacterial infections are continually developing resistance to conventional antibiotic agents, thereby prompting the search for bioactive compounds from plant parts that would serve as lead molecules in the discovery and development of new drugs. There is a need to explore sustainable, innovative, and safe natural therapeutic methods in preventing and managing AMR infections. Beta-lactamase secretion by bacteria is one of the main resistant mechanisms bacterial enzymes use to resist antibiotics. Hence, this study investigated the beta-lactamase inhibition potential of Salacia nitida and Rauvolfia vomitoria root extracts. Gas chromatography-mass spectrometry (GC-MS) analysis was carried out on the root extracts of S. nitida and R. vomitoria. Molecular docking was performed to determine the binding affinity and energy between class A beta-lactamase and selected bioactive compounds. Results from the beta-lactamase inhibition assay showed that S. nitida and R. vomitoria root extracts had 65.87% and 69.89% inhibitory activity, respectively, against the beta-lactamase enzyme, suggesting that these plant extracts have the potential to be used as a beta-lactamase inhibitor in combination with beta-lactam antibiotics. The GC-MS results revealed 10 bioactive compounds in S. nitida and 28 compounds in R. vomitoria. Molecular docking results showed favourable hydrogen bonds and Van der waal interactions between beta-lactamase and selected bioactive compounds. The findings suggest that these plant extracts possess significant beta-lactamase inhibition activity and this can further lend pharmacological support to their folklore uses as antibiotic agents. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/660Molecular dockingBeta-lactamaseAntimicrobialPhytoconstituentsBinding affinityBinding energy |
| spellingShingle | Ubamaka W Ike Osinachi Samdavid Okpara Chidi Ijeoma Nosiri G Enyinnaya Eze Chinedu Aguwamba Chinomso Friday Aaron Okamgba, C. O. S C Okereke GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal Plants UMYU Journal of Microbiology Research Molecular docking Beta-lactamase Antimicrobial Phytoconstituents Binding affinity Binding energy |
| title | GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal Plants |
| title_full | GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal Plants |
| title_fullStr | GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal Plants |
| title_full_unstemmed | GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal Plants |
| title_short | GC-MS profiling, Beta-lactamase Inhibition Assay, and Molecular Docking Studies on Selected Medicinal Plants |
| title_sort | gc ms profiling beta lactamase inhibition assay and molecular docking studies on selected medicinal plants |
| topic | Molecular docking Beta-lactamase Antimicrobial Phytoconstituents Binding affinity Binding energy |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/660 |
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