Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptides
Actinomycetes were isolated from mangrove soil samples and cultivated on starch casein nitrate (SCN) medium. The isolate was identified as Boudabousia marimammalium through 16S rRNA gene sequencing and phylogenetic analysis using MEGA X. Microscopic examination revealed filamentous mycelia with spir...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1574859/full |
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| author | Yalpi Karthik Srinivasa Krishnappa Manjula Ishwara Kalyani Ramakrishna D. Prabhudev S. H. Kumar K. M. Sanchit Thakur Laila A. Al-Shuraym Lamya Ahmed Alkeridis Ohud Muslat Aharthy Seham Sater Alhelaify Muntazir Mushtaq |
| author_facet | Yalpi Karthik Srinivasa Krishnappa Manjula Ishwara Kalyani Ramakrishna D. Prabhudev S. H. Kumar K. M. Sanchit Thakur Laila A. Al-Shuraym Lamya Ahmed Alkeridis Ohud Muslat Aharthy Seham Sater Alhelaify Muntazir Mushtaq |
| author_sort | Yalpi Karthik |
| collection | DOAJ |
| description | Actinomycetes were isolated from mangrove soil samples and cultivated on starch casein nitrate (SCN) medium. The isolate was identified as Boudabousia marimammalium through 16S rRNA gene sequencing and phylogenetic analysis using MEGA X. Microscopic examination revealed filamentous mycelia with spirally coiled spore chains bearing cylindrical, hairy spores with curved edges, consistent with the genus morphology. Crude protein was extracted, partially purified (11.52-fold), and quantified (0.195 mg/mL, 8.48% recovery). Antimicrobial activity was tested against a panel of bacterial pathogens, and anticancer potential was evaluated using PC3 prostate cancer cells. LC-MS was employed for compound identification. The crude protein extract exhibited significant antimicrobial activity against Proteus vulgaris (22 ± 0.6 mm), Salmonella typhimurium (15 ± 0.6 mm), Bacillus cereus (13 ± 0.6 mm), Pseudomonas aeruginosa (14 ± 0.6 mm), and Staphylococcus aureus (10 ± 0.6 mm). It also showed anticancer activity, causing 37.43% growth inhibition of PC3 cells at 200 µg. LC-MS analysis identified a dipeptide with a molecular weight of 351.45 Da, corresponding to Tryprostatin B, a known bioactive compound. The isolate B. marimammalium from mangrove soil produces bioactive peptides with dual antimicrobial and anticancer properties. |
| format | Article |
| id | doaj-art-0fff504b8e40442c8e238048a29caec1 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-0fff504b8e40442c8e238048a29caec12025-08-20T03:28:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15748591574859Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptidesYalpi Karthik0Srinivasa Krishnappa1Manjula Ishwara Kalyani2Ramakrishna D.3Prabhudev S. H.4Kumar K. M.5Sanchit Thakur6Laila A. Al-Shuraym7Lamya Ahmed Alkeridis8Ohud Muslat Aharthy9Seham Sater Alhelaify10Muntazir Mushtaq11Department of Studies and Research in Microbiology, Mangalore University, Kodagu, IndiaDepartment of Studies and Research in Biochemistry, Mangalore University, Kodagu, IndiaDepartment of Studies and Research in Microbiology, Mangalore University, Kodagu, IndiaDr. C.D Sagar Centre for Life Sciences, Department of Biotechnology, Dayananda Sagar College of Engineering, Dayananda Sagar Institutions, Bengaluru, IndiaDepartment of Studies and Research in Biochemistry, Mangalore University, Kodagu, IndiaDepartment of Bioinformatics, Pondicherry University, Pondicherry, IndiaMS Swaminathan School of Agriculture, Shoolini University, Solan, IndiaDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Biotechnology, Faculty of Science, Taif University, Taif, Saudi ArabiaDepartment of Biotechnology, Faculty of Science, Taif University, Taif, Saudi ArabiaMS Swaminathan School of Agriculture, Shoolini University, Solan, IndiaActinomycetes were isolated from mangrove soil samples and cultivated on starch casein nitrate (SCN) medium. The isolate was identified as Boudabousia marimammalium through 16S rRNA gene sequencing and phylogenetic analysis using MEGA X. Microscopic examination revealed filamentous mycelia with spirally coiled spore chains bearing cylindrical, hairy spores with curved edges, consistent with the genus morphology. Crude protein was extracted, partially purified (11.52-fold), and quantified (0.195 mg/mL, 8.48% recovery). Antimicrobial activity was tested against a panel of bacterial pathogens, and anticancer potential was evaluated using PC3 prostate cancer cells. LC-MS was employed for compound identification. The crude protein extract exhibited significant antimicrobial activity against Proteus vulgaris (22 ± 0.6 mm), Salmonella typhimurium (15 ± 0.6 mm), Bacillus cereus (13 ± 0.6 mm), Pseudomonas aeruginosa (14 ± 0.6 mm), and Staphylococcus aureus (10 ± 0.6 mm). It also showed anticancer activity, causing 37.43% growth inhibition of PC3 cells at 200 µg. LC-MS analysis identified a dipeptide with a molecular weight of 351.45 Da, corresponding to Tryprostatin B, a known bioactive compound. The isolate B. marimammalium from mangrove soil produces bioactive peptides with dual antimicrobial and anticancer properties.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1574859/fullactinomycetesantimicrobial peptidesanticancer peptidesBoudabousia marimammaliummangrove habitat |
| spellingShingle | Yalpi Karthik Srinivasa Krishnappa Manjula Ishwara Kalyani Ramakrishna D. Prabhudev S. H. Kumar K. M. Sanchit Thakur Laila A. Al-Shuraym Lamya Ahmed Alkeridis Ohud Muslat Aharthy Seham Sater Alhelaify Muntazir Mushtaq Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptides Frontiers in Microbiology actinomycetes antimicrobial peptides anticancer peptides Boudabousia marimammalium mangrove habitat |
| title | Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptides |
| title_full | Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptides |
| title_fullStr | Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptides |
| title_full_unstemmed | Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptides |
| title_short | Isolation and characterization of Boudabousia marimammalium from a mangrove habitat: identification of dual-function antimicrobial and anticancer peptides |
| title_sort | isolation and characterization of boudabousia marimammalium from a mangrove habitat identification of dual function antimicrobial and anticancer peptides |
| topic | actinomycetes antimicrobial peptides anticancer peptides Boudabousia marimammalium mangrove habitat |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1574859/full |
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