Transcriptome responses to Ralstonia solanacearum infection in tetraploid potato
Potato (Solanum tuberosum) is an important global food source, the growth of which can be severely impacted by Ralstonia solanacearum bacterial infection. Despite extensive research, the molecular mechanisms of potato resistance to this pathogen are imperfectly known. Huashu No. 12, a tetraploid pot...
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402500283X |
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| author | Zhuo Chen Shunwei Shao Xi Zhu Yu Zhang Zhendong Lan Hui Jin |
| author_facet | Zhuo Chen Shunwei Shao Xi Zhu Yu Zhang Zhendong Lan Hui Jin |
| author_sort | Zhuo Chen |
| collection | DOAJ |
| description | Potato (Solanum tuberosum) is an important global food source, the growth of which can be severely impacted by Ralstonia solanacearum bacterial infection. Despite extensive research, the molecular mechanisms of potato resistance to this pathogen are imperfectly known. Huashu No. 12, a tetraploid potato genotype, is highly resistant to R. solanacearum. We inoculate Huashu No. 12 and Longshu No. 7 (highly susceptible to R. solanacearum) with R. solanacearum to compare disease resistance in these two potato varieties. Huashu No. 12 has significantly higher resistance to R. solanacearum infection than Longshu No. 7, with increased lignin content, and an abundance of callose and strong autofluorescence in the phloem sieve tube. Enzymes (e.g., superoxide dismutase, catalase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase) contribute to R. solanacearum resistance in Huashu No. 12. Transcriptome sequencing reveals 659 differentially expressed genes between the two varieties, with the ethylene responsive factor family containing the most differentially expressed genes. Gene ontology and KEGG analyses provided further insights into the genetic basis and molecular mechanisms underlying plant defense against R. solanacearum disease. By demonstrating the importance of enzymes and differential gene expression in Huashu No. 12 resistance to R. solanacearum infection, the breeding of disease-resistant potato becomes increasingly feasible. |
| format | Article |
| id | doaj-art-0ff22d32ce0c44778df3f67610105909 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-0ff22d32ce0c44778df3f676101059092025-02-02T05:28:31ZengElsevierHeliyon2405-84402025-01-01112e41903Transcriptome responses to Ralstonia solanacearum infection in tetraploid potatoZhuo Chen0Shunwei Shao1Xi Zhu2Yu Zhang3Zhendong Lan4Hui Jin5Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China; Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China, Zhanjiang, ChinaKey Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China; College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China; Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China, Zhanjiang, ChinaKey Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China; Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China, Zhanjiang, ChinaKey Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China; College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China; Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China, Zhanjiang, China; Corresponding author. Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China.Potato (Solanum tuberosum) is an important global food source, the growth of which can be severely impacted by Ralstonia solanacearum bacterial infection. Despite extensive research, the molecular mechanisms of potato resistance to this pathogen are imperfectly known. Huashu No. 12, a tetraploid potato genotype, is highly resistant to R. solanacearum. We inoculate Huashu No. 12 and Longshu No. 7 (highly susceptible to R. solanacearum) with R. solanacearum to compare disease resistance in these two potato varieties. Huashu No. 12 has significantly higher resistance to R. solanacearum infection than Longshu No. 7, with increased lignin content, and an abundance of callose and strong autofluorescence in the phloem sieve tube. Enzymes (e.g., superoxide dismutase, catalase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase) contribute to R. solanacearum resistance in Huashu No. 12. Transcriptome sequencing reveals 659 differentially expressed genes between the two varieties, with the ethylene responsive factor family containing the most differentially expressed genes. Gene ontology and KEGG analyses provided further insights into the genetic basis and molecular mechanisms underlying plant defense against R. solanacearum disease. By demonstrating the importance of enzymes and differential gene expression in Huashu No. 12 resistance to R. solanacearum infection, the breeding of disease-resistant potato becomes increasingly feasible.http://www.sciencedirect.com/science/article/pii/S240584402500283XPotatoRalstonia solanacearumRNA-SeqDisease resistanceDifferentially expressed genes |
| spellingShingle | Zhuo Chen Shunwei Shao Xi Zhu Yu Zhang Zhendong Lan Hui Jin Transcriptome responses to Ralstonia solanacearum infection in tetraploid potato Heliyon Potato Ralstonia solanacearum RNA-Seq Disease resistance Differentially expressed genes |
| title | Transcriptome responses to Ralstonia solanacearum infection in tetraploid potato |
| title_full | Transcriptome responses to Ralstonia solanacearum infection in tetraploid potato |
| title_fullStr | Transcriptome responses to Ralstonia solanacearum infection in tetraploid potato |
| title_full_unstemmed | Transcriptome responses to Ralstonia solanacearum infection in tetraploid potato |
| title_short | Transcriptome responses to Ralstonia solanacearum infection in tetraploid potato |
| title_sort | transcriptome responses to ralstonia solanacearum infection in tetraploid potato |
| topic | Potato Ralstonia solanacearum RNA-Seq Disease resistance Differentially expressed genes |
| url | http://www.sciencedirect.com/science/article/pii/S240584402500283X |
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