Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriers
Unravelling the features of the whole-body response to acute stress exposures is critical to understand this neglected building block of chronic stress. A single acute stress exposure rapidly modulates gut-brain axis signalling including intestinal permeability, but the mechanisms are unclear. Micro...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Brain, Behavior, & Immunity - Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266635462500050X |
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| author | Cristina Rosell-Cardona Sarah-Jane Leigh Emily Knox Emanuela Tirelli Joshua M. Lyte Michael S. Goodson Nancy Kelley-Loughnane Maria R. Aburto John F. Cryan Gerard Clarke |
| author_facet | Cristina Rosell-Cardona Sarah-Jane Leigh Emily Knox Emanuela Tirelli Joshua M. Lyte Michael S. Goodson Nancy Kelley-Loughnane Maria R. Aburto John F. Cryan Gerard Clarke |
| author_sort | Cristina Rosell-Cardona |
| collection | DOAJ |
| description | Unravelling the features of the whole-body response to acute stress exposures is critical to understand this neglected building block of chronic stress. A single acute stress exposure rapidly modulates gut-brain axis signalling including intestinal permeability, but the mechanisms are unclear. Microbially-produced metabolites such as short-chain fatty acids (SCFA) are key effectors within the gut-brain axis which can affect gut and brain function. The aim of this work is to determine if acute stress regulates SCFA production in the gut and to understand the associated implications for gastrointestinal and brain barrier function. Stress reduced caecal SCFA concentrations, primarily butyrate and acetate. These SCFAs prevented LPS-induced disruption of gut and brain barrier function in a dose-dependent manner in in vitro models. This functional protection was associated with altered tight-junction abundance and morphology. These results provide a better understanding of the role SCFAs have on barriers following acute stress. |
| format | Article |
| id | doaj-art-0fe72f62121843b39d754df7e861e4b6 |
| institution | DOAJ |
| issn | 2666-3546 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain, Behavior, & Immunity - Health |
| spelling | doaj-art-0fe72f62121843b39d754df7e861e4b62025-08-20T03:21:15ZengElsevierBrain, Behavior, & Immunity - Health2666-35462025-07-014610099210.1016/j.bbih.2025.100992Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriersCristina Rosell-Cardona0Sarah-Jane Leigh1Emily Knox2Emanuela Tirelli3Joshua M. Lyte4Michael S. Goodson5Nancy Kelley-Loughnane6Maria R. Aburto7John F. Cryan8Gerard Clarke9APC Microbiome Ireland, University College Cork, Cork, Ireland; Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland; Department of Psychiatry and Neurobehavioural Science, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, Ireland; Department of Anatomy and Neuroscience, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, Ireland711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, USA711th Human Performance Wing, Air Force Research Laboratory, Wright-Patterson Air Force Base, Dayton, OH, USAAPC Microbiome Ireland, University College Cork, Cork, Ireland; Department of Anatomy and Neuroscience, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, Ireland; Department of Anatomy and Neuroscience, University College Cork, Cork, IrelandAPC Microbiome Ireland, University College Cork, Cork, Ireland; Department of Psychiatry and Neurobehavioural Science, University College Cork, Cork, Ireland; Corresponding author. 1.15 Biosciences Building, Dept of Psychiatry and Neurobehavioural Science, University College Cork, Cork, Ireland. Tel.: +353 (0)21 4901408; fax: +353 (0)21 4901722.Unravelling the features of the whole-body response to acute stress exposures is critical to understand this neglected building block of chronic stress. A single acute stress exposure rapidly modulates gut-brain axis signalling including intestinal permeability, but the mechanisms are unclear. Microbially-produced metabolites such as short-chain fatty acids (SCFA) are key effectors within the gut-brain axis which can affect gut and brain function. The aim of this work is to determine if acute stress regulates SCFA production in the gut and to understand the associated implications for gastrointestinal and brain barrier function. Stress reduced caecal SCFA concentrations, primarily butyrate and acetate. These SCFAs prevented LPS-induced disruption of gut and brain barrier function in a dose-dependent manner in in vitro models. This functional protection was associated with altered tight-junction abundance and morphology. These results provide a better understanding of the role SCFAs have on barriers following acute stress.http://www.sciencedirect.com/science/article/pii/S266635462500050XAcute stressShort-chain fatty acidsMicrobiota-gut-brain axisBarriersMicrobial metabolites |
| spellingShingle | Cristina Rosell-Cardona Sarah-Jane Leigh Emily Knox Emanuela Tirelli Joshua M. Lyte Michael S. Goodson Nancy Kelley-Loughnane Maria R. Aburto John F. Cryan Gerard Clarke Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriers Brain, Behavior, & Immunity - Health Acute stress Short-chain fatty acids Microbiota-gut-brain axis Barriers Microbial metabolites |
| title | Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriers |
| title_full | Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriers |
| title_fullStr | Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriers |
| title_full_unstemmed | Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriers |
| title_short | Acute stress-induced alterations in short-chain fatty acids: Implications for the intestinal and blood brain barriers |
| title_sort | acute stress induced alterations in short chain fatty acids implications for the intestinal and blood brain barriers |
| topic | Acute stress Short-chain fatty acids Microbiota-gut-brain axis Barriers Microbial metabolites |
| url | http://www.sciencedirect.com/science/article/pii/S266635462500050X |
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