Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry
Metronidazole (MNZ) is one of the most commonly used antibiotics in the food industry. High levels in food can lead to the development of antimicrobial resistance in humans, so it is important to monitor its levels in food. In the context of legal proceedings, it is frequently necessary to re-examin...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524009751 |
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| author | Mikhail Vokuev Artem Melekhin Anastasia Frolova Anton Bairov Igor Rodin Victor Tishchenko |
| author_facet | Mikhail Vokuev Artem Melekhin Anastasia Frolova Anton Bairov Igor Rodin Victor Tishchenko |
| author_sort | Mikhail Vokuev |
| collection | DOAJ |
| description | Metronidazole (MNZ) is one of the most commonly used antibiotics in the food industry. High levels in food can lead to the development of antimicrobial resistance in humans, so it is important to monitor its levels in food. In the context of legal proceedings, it is frequently necessary to re-examine samples after an extended period of time. It is therefore crucial to ensure that the analytes in question do not degrade during the storage period. In this study, HPLC-MS/MS approach was validated and used to analyze levels of MNZ and its hydroxy metabolite in chicken eggs during storage in the refrigerator (+4 °C) and in the freezer (−20 °C) for 3 months. An analyte solution was administered to hens to obtain eggs containing MNZ and hydroxymetronidazole (MNZ-OH). The dependence of the analyte content in eggs as a function of time after sampling was also investigated. |
| format | Article |
| id | doaj-art-0fdb436990fe4dcea44ed17dbffe759f |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-0fdb436990fe4dcea44ed17dbffe759f2025-02-12T05:31:54ZengElsevierFood Chemistry: X2590-15752025-01-0125102087Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometryMikhail Vokuev0Artem Melekhin1Anastasia Frolova2Anton Bairov3Igor Rodin4Victor Tishchenko5Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaFederal Centre for Animal Health, 600901 Vladimir, Russia; Corresponding author.Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaFederal Centre for Animal Health, 600901 Vladimir, RussiaDepartment of Chemistry, Lomonosov Moscow State University, 119991 Moscow, RussiaFederal Centre for Animal Health, 600901 Vladimir, RussiaMetronidazole (MNZ) is one of the most commonly used antibiotics in the food industry. High levels in food can lead to the development of antimicrobial resistance in humans, so it is important to monitor its levels in food. In the context of legal proceedings, it is frequently necessary to re-examine samples after an extended period of time. It is therefore crucial to ensure that the analytes in question do not degrade during the storage period. In this study, HPLC-MS/MS approach was validated and used to analyze levels of MNZ and its hydroxy metabolite in chicken eggs during storage in the refrigerator (+4 °C) and in the freezer (−20 °C) for 3 months. An analyte solution was administered to hens to obtain eggs containing MNZ and hydroxymetronidazole (MNZ-OH). The dependence of the analyte content in eggs as a function of time after sampling was also investigated.http://www.sciencedirect.com/science/article/pii/S2590157524009751MetronidazoleHydroxymetronidazoleLiquid chromatographyTandem mass spectrometry |
| spellingShingle | Mikhail Vokuev Artem Melekhin Anastasia Frolova Anton Bairov Igor Rodin Victor Tishchenko Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry Food Chemistry: X Metronidazole Hydroxymetronidazole Liquid chromatography Tandem mass spectrometry |
| title | Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry |
| title_full | Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry |
| title_fullStr | Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry |
| title_full_unstemmed | Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry |
| title_short | Storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry |
| title_sort | storage stability study of metronidazole and hydroxymetronidazole in chicken eggs by liquid chromatography tandem mass spectrometry |
| topic | Metronidazole Hydroxymetronidazole Liquid chromatography Tandem mass spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524009751 |
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