Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep Breeds
Organisms have the capacity to detect day–night fluctuations through oscillators regulated by circadian clock genes, which are crucial for regulating various biological processes. Numerous studies have demonstrated a marked association between these genes and various growth traits of sheep. This stu...
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2025-01-01
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| author | Ziteng Wang Xiaohua Yi Mengzhe Yang Xiuzhu Sun Shuhui Wang |
| author_facet | Ziteng Wang Xiaohua Yi Mengzhe Yang Xiuzhu Sun Shuhui Wang |
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| description | Organisms have the capacity to detect day–night fluctuations through oscillators regulated by circadian clock genes, which are crucial for regulating various biological processes. Numerous studies have demonstrated a marked association between these genes and various growth traits of sheep. This study identified polymorphisms at 23 potential loci within five clock genes in four Chinese sheep breeds. Only two polymorphic insertion/deletions (InDels) were detected in <i>CLOCK</i> and <i>PER3</i> genes, respectively. The distribution of these two loci in four Chinese sheep breeds and their association with growth traits were further explored. A 12 bp deletion was found in the intron of the <i>CLOCK</i> gene (rs604230640), which was significantly associated with body height (<i>p</i> < 0.05), body oblique length (<i>p</i> < 0.05) and cannon girth (<i>p</i> < 0.05) in Hu sheep (HS). A 22 bp insertion in the intron of the <i>PER3</i> gene (rs600537720) with a dominant genotype of insertion/insertion (II) was found to have a significant association with chest depth (<i>p</i> < 0.05) in Small-Tail Han sheep (STHS), tail width (<i>p</i> < 0.05) in Tong Sheep (TS), and in Lanzhou fat-tailed sheep (LFTS). In conclusion, this study has elucidated the polymorphisms of <i>CLOCK</i> and <i>PER3</i> genes and has examined the influence of these two genes on the growth traits of sheep. Concurrently, the two molecular markers identified in <i>CLOCK</i> and <i>PER3</i> could potentially serve in the marker-assisted selection of growing-related traits in local Chinese sheep breeds. |
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| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-0fd6f851008c4adaa11a1e229909dae72025-01-24T13:52:04ZengMDPI AGVeterinary Sciences2306-73812025-01-011213910.3390/vetsci12010039Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep BreedsZiteng Wang0Xiaohua Yi1Mengzhe Yang2Xiuzhu Sun3Shuhui Wang4College of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaCollege of Grassland Agriculture, Northwest A&F University, Yangling 712100, ChinaCollege of Animal Science and Technology, Northwest A&F University, Yangling 712100, ChinaOrganisms have the capacity to detect day–night fluctuations through oscillators regulated by circadian clock genes, which are crucial for regulating various biological processes. Numerous studies have demonstrated a marked association between these genes and various growth traits of sheep. This study identified polymorphisms at 23 potential loci within five clock genes in four Chinese sheep breeds. Only two polymorphic insertion/deletions (InDels) were detected in <i>CLOCK</i> and <i>PER3</i> genes, respectively. The distribution of these two loci in four Chinese sheep breeds and their association with growth traits were further explored. A 12 bp deletion was found in the intron of the <i>CLOCK</i> gene (rs604230640), which was significantly associated with body height (<i>p</i> < 0.05), body oblique length (<i>p</i> < 0.05) and cannon girth (<i>p</i> < 0.05) in Hu sheep (HS). A 22 bp insertion in the intron of the <i>PER3</i> gene (rs600537720) with a dominant genotype of insertion/insertion (II) was found to have a significant association with chest depth (<i>p</i> < 0.05) in Small-Tail Han sheep (STHS), tail width (<i>p</i> < 0.05) in Tong Sheep (TS), and in Lanzhou fat-tailed sheep (LFTS). In conclusion, this study has elucidated the polymorphisms of <i>CLOCK</i> and <i>PER3</i> genes and has examined the influence of these two genes on the growth traits of sheep. Concurrently, the two molecular markers identified in <i>CLOCK</i> and <i>PER3</i> could potentially serve in the marker-assisted selection of growing-related traits in local Chinese sheep breeds.https://www.mdpi.com/2306-7381/12/1/39sheepcircadian clock geneinsertion/deletion (InDel)growth traitsassociation |
| spellingShingle | Ziteng Wang Xiaohua Yi Mengzhe Yang Xiuzhu Sun Shuhui Wang Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep Breeds Veterinary Sciences sheep circadian clock gene insertion/deletion (InDel) growth traits association |
| title | Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep Breeds |
| title_full | Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep Breeds |
| title_fullStr | Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep Breeds |
| title_full_unstemmed | Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep Breeds |
| title_short | Detection of Insertion/Deletions (InDel) Within Five Clock Genes and Their Associations with Growth Traits in Four Chinese Sheep Breeds |
| title_sort | detection of insertion deletions indel within five clock genes and their associations with growth traits in four chinese sheep breeds |
| topic | sheep circadian clock gene insertion/deletion (InDel) growth traits association |
| url | https://www.mdpi.com/2306-7381/12/1/39 |
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