Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol Background

During the postpartum period, domestic ruminants suffer elevated endogenous cortisol levels, which are associated with an increased risk of uterine infections. Selenium is a trace mineral nutrient with beneficial impacts on animals. The study aimed to investigate whether selenium yeast (SeY) could a...

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Main Authors: Changning Yuan, Hanqing Li, Min Zhang, Zhihao Wang, Junsheng Dong, Luying Cui, Long Guo, Kangjun Liu, Jianji Li, Heng Wang
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/5/693
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author Changning Yuan
Hanqing Li
Min Zhang
Zhihao Wang
Junsheng Dong
Luying Cui
Long Guo
Kangjun Liu
Jianji Li
Heng Wang
author_facet Changning Yuan
Hanqing Li
Min Zhang
Zhihao Wang
Junsheng Dong
Luying Cui
Long Guo
Kangjun Liu
Jianji Li
Heng Wang
author_sort Changning Yuan
collection DOAJ
description During the postpartum period, domestic ruminants suffer elevated endogenous cortisol levels, which are associated with an increased risk of uterine infections. Selenium is a trace mineral nutrient with beneficial impacts on animals. The study aimed to investigate whether selenium yeast (SeY) could attenuate <i>Escherichia coli</i> (<i>E. coli</i>)-induced endometrial injury in goats with high cortisol background. Goats were examined after oral SeY administration for 21 days and were treated with glacial acetic acid, <i>E. coli</i>, and hydrocortisone to establish an endometritis model with high cortisol background. The results showed that endometrial injury caused by <i>E. coli</i> was aggravated under high cortisol background. Supplementation with SeY alleviated endometrial inflammation and serum LDH content. The mRNA expression of pro-inflammatory cytokines and defensin beta 2 and the phosphorylation level of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-b (NF-κB) signaling pathways were decreased by SeY supplementation. Total antioxidant capacity and antioxidant enzymes activities were increased by SeY supplementation, but malondialdehyde and 4-hydroxynonenal content were decreased. Moreover, nuclear factor erythroid-2 related factor 2 (NRF2) in the nucleus, heme oxygenase-1, and NAD(P)H quinone dehydrogenase 1 were increased by SeY supplementation. So, supplementation with SeY alleviated <i>E. coli</i>-induced endometritis in goats by activating the NRF2 pathway and inhibiting the activation of the MAPK and NF-κB pathways under postpartum stress.
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spelling doaj-art-0fc4a42ba9fb4e93ad86c5fb055a3b952025-08-20T02:04:34ZengMDPI AGAnimals2076-26152025-02-0115569310.3390/ani15050693Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol BackgroundChangning Yuan0Hanqing Li1Min Zhang2Zhihao Wang3Junsheng Dong4Luying Cui5Long Guo6Kangjun Liu7Jianji Li8Heng Wang9College of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Shandong Agricultural University, Taian 271018, ChinaCollege of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaPancreatic Center, Department of Gastroenterology, Yangzhou Key Laboratory of Pancreatic Disease, The Afliated Hospital of Yangzhou University, Yangzhou University, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, ChinaDuring the postpartum period, domestic ruminants suffer elevated endogenous cortisol levels, which are associated with an increased risk of uterine infections. Selenium is a trace mineral nutrient with beneficial impacts on animals. The study aimed to investigate whether selenium yeast (SeY) could attenuate <i>Escherichia coli</i> (<i>E. coli</i>)-induced endometrial injury in goats with high cortisol background. Goats were examined after oral SeY administration for 21 days and were treated with glacial acetic acid, <i>E. coli</i>, and hydrocortisone to establish an endometritis model with high cortisol background. The results showed that endometrial injury caused by <i>E. coli</i> was aggravated under high cortisol background. Supplementation with SeY alleviated endometrial inflammation and serum LDH content. The mRNA expression of pro-inflammatory cytokines and defensin beta 2 and the phosphorylation level of the mitogen-activated protein kinase (MAPK) and nuclear factor kappa-b (NF-κB) signaling pathways were decreased by SeY supplementation. Total antioxidant capacity and antioxidant enzymes activities were increased by SeY supplementation, but malondialdehyde and 4-hydroxynonenal content were decreased. Moreover, nuclear factor erythroid-2 related factor 2 (NRF2) in the nucleus, heme oxygenase-1, and NAD(P)H quinone dehydrogenase 1 were increased by SeY supplementation. So, supplementation with SeY alleviated <i>E. coli</i>-induced endometritis in goats by activating the NRF2 pathway and inhibiting the activation of the MAPK and NF-κB pathways under postpartum stress.https://www.mdpi.com/2076-2615/15/5/693blood flow resistanceendometriuminflammationoxidative damagetrace elements
spellingShingle Changning Yuan
Hanqing Li
Min Zhang
Zhihao Wang
Junsheng Dong
Luying Cui
Long Guo
Kangjun Liu
Jianji Li
Heng Wang
Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol Background
Animals
blood flow resistance
endometrium
inflammation
oxidative damage
trace elements
title Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol Background
title_full Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol Background
title_fullStr Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol Background
title_full_unstemmed Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol Background
title_short Selenium Yeast Alleviates <i>Escherichia coli</i>-Induced Endometritis in Goats Under High Cortisol Background
title_sort selenium yeast alleviates i escherichia coli i induced endometritis in goats under high cortisol background
topic blood flow resistance
endometrium
inflammation
oxidative damage
trace elements
url https://www.mdpi.com/2076-2615/15/5/693
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