Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributes
This study explored the probiotic potential, antimicrobial, safety, and anti-biofilm properties of Lacticaseibacillus rhamnosus SMHA30. The strain exhibited the highest cell viability at pH 4.5 (8.16 log colony forming unit (CFU)/mL) and the lowest at pH 2.5 (7.08 log CFU/mL). In bile stability test...
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Elsevier
2025-06-01
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| Series: | Applied Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772502225001854 |
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| author | Behrooz Alizadeh Behbahani Hassan Barzegar Morteza Taki |
| author_facet | Behrooz Alizadeh Behbahani Hassan Barzegar Morteza Taki |
| author_sort | Behrooz Alizadeh Behbahani |
| collection | DOAJ |
| description | This study explored the probiotic potential, antimicrobial, safety, and anti-biofilm properties of Lacticaseibacillus rhamnosus SMHA30. The strain exhibited the highest cell viability at pH 4.5 (8.16 log colony forming unit (CFU)/mL) and the lowest at pH 2.5 (7.08 log CFU/mL). In bile stability tests, cell viability remained consistent across 0.30% and 0.50% bile concentrations (8.55 and 8.41 log CFU/mL). L. rhamnosus SMHA30 demonstrated a notable surface hydrophobicity of 57.61%, an auto-aggregation rate of 47.81%, and a co-aggregation rate of 50.50%, while adhering to Caco-2 cells at a rate of 12.95%. The strain effectively inhibited Listeria monocytogenes, with competition, inhibition, and displacement rates of 52.56%, 49.95%, and 24.95%, respectively. Furthermore, L. rhamnosus SMHA30 showed antioxidant activity with 2,2,-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging abilities of 53.60% and 61.43%, respectively, and demonstrated substantial anti-cancer effects on MCF-7, HeLa, and HT-29 cells. Additionally, the strain had a cholesterol assimilation rate of 52.95% and was resistant to ampicillin while being susceptible to other antibiotics. The strongest antibacterial effect was noted against L. monocytogenes, while the weakest was observed against Salmonella typhi. The minimum inhibitory concentration (MIC) was determined to be 7.81 mg/mL for L. monocytogenes and 125 mg/ mL for S. typhi. No biogenic amine production, DNase activity, or hemolysis was observed. In this research, Gaussian Process Regression (GPR) method was used to predict acid and bile tolerance. The results indicated that GPR can predict both the output parameters with high accuracy. Overall, L. rhamnosus SMHA30 represents a valuable candidate for further investigation in probiotic development and biofilm management strategies. |
| format | Article |
| id | doaj-art-0fc48ff1dfc540cc84b4bf8e247780f1 |
| institution | Kabale University |
| issn | 2772-5022 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Food Research |
| spelling | doaj-art-0fc48ff1dfc540cc84b4bf8e247780f12025-08-20T03:45:18ZengElsevierApplied Food Research2772-50222025-06-015110088210.1016/j.afres.2025.100882Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributesBehrooz Alizadeh Behbahani0Hassan Barzegar1Morteza Taki2Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran; Corresponding author.Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, IranDepartment of Agricultural Machinery and Mechanization Engineering, Faculty of Agricultural Engineering and Rural Development, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, IranThis study explored the probiotic potential, antimicrobial, safety, and anti-biofilm properties of Lacticaseibacillus rhamnosus SMHA30. The strain exhibited the highest cell viability at pH 4.5 (8.16 log colony forming unit (CFU)/mL) and the lowest at pH 2.5 (7.08 log CFU/mL). In bile stability tests, cell viability remained consistent across 0.30% and 0.50% bile concentrations (8.55 and 8.41 log CFU/mL). L. rhamnosus SMHA30 demonstrated a notable surface hydrophobicity of 57.61%, an auto-aggregation rate of 47.81%, and a co-aggregation rate of 50.50%, while adhering to Caco-2 cells at a rate of 12.95%. The strain effectively inhibited Listeria monocytogenes, with competition, inhibition, and displacement rates of 52.56%, 49.95%, and 24.95%, respectively. Furthermore, L. rhamnosus SMHA30 showed antioxidant activity with 2,2,-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging abilities of 53.60% and 61.43%, respectively, and demonstrated substantial anti-cancer effects on MCF-7, HeLa, and HT-29 cells. Additionally, the strain had a cholesterol assimilation rate of 52.95% and was resistant to ampicillin while being susceptible to other antibiotics. The strongest antibacterial effect was noted against L. monocytogenes, while the weakest was observed against Salmonella typhi. The minimum inhibitory concentration (MIC) was determined to be 7.81 mg/mL for L. monocytogenes and 125 mg/ mL for S. typhi. No biogenic amine production, DNase activity, or hemolysis was observed. In this research, Gaussian Process Regression (GPR) method was used to predict acid and bile tolerance. The results indicated that GPR can predict both the output parameters with high accuracy. Overall, L. rhamnosus SMHA30 represents a valuable candidate for further investigation in probiotic development and biofilm management strategies.http://www.sciencedirect.com/science/article/pii/S2772502225001854Anti-biofilmGaussian process regressionLacticaseibacillus rhamnosus SMHA30Probiotic potential |
| spellingShingle | Behrooz Alizadeh Behbahani Hassan Barzegar Morteza Taki Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributes Applied Food Research Anti-biofilm Gaussian process regression Lacticaseibacillus rhamnosus SMHA30 Probiotic potential |
| title | Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributes |
| title_full | Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributes |
| title_fullStr | Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributes |
| title_full_unstemmed | Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributes |
| title_short | Isolation of Lacticaseibacillus rhamnosus SMHA30 from traditional yogurt: A probiotic with antimicrobial, anti-biofilm, and safety attributes |
| title_sort | isolation of lacticaseibacillus rhamnosus smha30 from traditional yogurt a probiotic with antimicrobial anti biofilm and safety attributes |
| topic | Anti-biofilm Gaussian process regression Lacticaseibacillus rhamnosus SMHA30 Probiotic potential |
| url | http://www.sciencedirect.com/science/article/pii/S2772502225001854 |
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