Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterization

Agave, a plant that is primarily used for the production of alcoholic beverages (tequila), and plays a significant role in the culture and economy of Mexico. Recently, Agave has been introduced in Thailand, and has predominantly been used for landscaping and as a firebreak, owing to its resilience....

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Main Authors: Antika Boondaeng, Chanaporn Trakunjae, Nanthavut Niyomvong
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225001362
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author Antika Boondaeng
Chanaporn Trakunjae
Nanthavut Niyomvong
author_facet Antika Boondaeng
Chanaporn Trakunjae
Nanthavut Niyomvong
author_sort Antika Boondaeng
collection DOAJ
description Agave, a plant that is primarily used for the production of alcoholic beverages (tequila), and plays a significant role in the culture and economy of Mexico. Recently, Agave has been introduced in Thailand, and has predominantly been used for landscaping and as a firebreak, owing to its resilience. However, the water-soluble carbohydrates in Agave stems are an excellent substrate for fermentation processes. In this study, we aimed to utilize Agave sap for vinegar production via a sequential fermentation process using the yeast strain, Saccharomyces cerevisiae var. burgundy and S. cerevisiae var. montache in combination with acetic acid bacteria. The results showed that the combination of Agave vinegar and pineapple juice had the highest acetic acid concentration at 5.50 ± 0.09%, with the phenolic acid content and antioxidant activity of 359.12 ± 6.31 mg GAE/L and 176.21 ± 1.19 µg TE/g, respectively. Conversely, Agave vinegar diluted with water exhibited an acetic acid concentration of 2.37 ± 0.02%, with a phenolic acid content and antioxidant activity of 152.79 ± 2.68 mg GAE/L and 60.81 ± 3.78 µg TE/g, respectively. Through the results of this study we aim to augment the value of Agave, potentially transforming them into commercially sustainable crops.
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spelling doaj-art-0fa9d52bfbea488db55942f1ad9682ff2025-08-20T02:39:58ZengElsevierApplied Food Research2772-50222025-06-015110082610.1016/j.afres.2025.100826Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterizationAntika Boondaeng0Chanaporn Trakunjae1Nanthavut Niyomvong2Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok 10900, ThailandKasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok 10900, ThailandDepartment of Biology and Biotechnology, Faculty of Science and Technology, Nakhon Sawan Rajabhat University, Nakhon Sawan 60000, Thailand; Science Center, Nakhon Sawan Rajabhat University, Nakhon Sawan 60000, Thailand; Corresponding author.Agave, a plant that is primarily used for the production of alcoholic beverages (tequila), and plays a significant role in the culture and economy of Mexico. Recently, Agave has been introduced in Thailand, and has predominantly been used for landscaping and as a firebreak, owing to its resilience. However, the water-soluble carbohydrates in Agave stems are an excellent substrate for fermentation processes. In this study, we aimed to utilize Agave sap for vinegar production via a sequential fermentation process using the yeast strain, Saccharomyces cerevisiae var. burgundy and S. cerevisiae var. montache in combination with acetic acid bacteria. The results showed that the combination of Agave vinegar and pineapple juice had the highest acetic acid concentration at 5.50 ± 0.09%, with the phenolic acid content and antioxidant activity of 359.12 ± 6.31 mg GAE/L and 176.21 ± 1.19 µg TE/g, respectively. Conversely, Agave vinegar diluted with water exhibited an acetic acid concentration of 2.37 ± 0.02%, with a phenolic acid content and antioxidant activity of 152.79 ± 2.68 mg GAE/L and 60.81 ± 3.78 µg TE/g, respectively. Through the results of this study we aim to augment the value of Agave, potentially transforming them into commercially sustainable crops.http://www.sciencedirect.com/science/article/pii/S2772502225001362Agave sapVinegarSequential fermentationPhenolic compoundAntioxidant
spellingShingle Antika Boondaeng
Chanaporn Trakunjae
Nanthavut Niyomvong
Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterization
Applied Food Research
Agave sap
Vinegar
Sequential fermentation
Phenolic compound
Antioxidant
title Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterization
title_full Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterization
title_fullStr Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterization
title_full_unstemmed Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterization
title_short Optimization of Agave-based pineapple vinegar fermentation through sequential fermentation and chemical characterization
title_sort optimization of agave based pineapple vinegar fermentation through sequential fermentation and chemical characterization
topic Agave sap
Vinegar
Sequential fermentation
Phenolic compound
Antioxidant
url http://www.sciencedirect.com/science/article/pii/S2772502225001362
work_keys_str_mv AT antikaboondaeng optimizationofagavebasedpineapplevinegarfermentationthroughsequentialfermentationandchemicalcharacterization
AT chanaporntrakunjae optimizationofagavebasedpineapplevinegarfermentationthroughsequentialfermentationandchemicalcharacterization
AT nanthavutniyomvong optimizationofagavebasedpineapplevinegarfermentationthroughsequentialfermentationandchemicalcharacterization