Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analyses
Pinus roxburghii and Pinus wallichiana are traditionally plants used for various medicinal purposes worldwide. The present study focused on evaluating the phytochemical compounds and the free radical scavenging potential in various parts of P. roxburghii and P. wallichiana through in vitro and in si...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Cogent Food & Agriculture |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/23311932.2024.2403648 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850247657861152768 |
|---|---|
| author | Saba Mehmood Arshad Mehmood Abbasi Hazrat Hussain Sabaz Ali Khan Hayfa Habes Almutairi Ahmed Mahmoud Ismail Mohamed S. Al Saikhan Hossam S. El-Beltagi |
| author_facet | Saba Mehmood Arshad Mehmood Abbasi Hazrat Hussain Sabaz Ali Khan Hayfa Habes Almutairi Ahmed Mahmoud Ismail Mohamed S. Al Saikhan Hossam S. El-Beltagi |
| author_sort | Saba Mehmood |
| collection | DOAJ |
| description | Pinus roxburghii and Pinus wallichiana are traditionally plants used for various medicinal purposes worldwide. The present study focused on evaluating the phytochemical compounds and the free radical scavenging potential in various parts of P. roxburghii and P. wallichiana through in vitro and in silico study. Crude extract of the fresh leaves was obtained using methanol while steam distillation method was used for the extraction of the essential oil. The phytochemicals were determined by standard methods. Antioxidant Potential of the phytochemicals was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), Hydroxyl radical (OH) inhibition, Ferric ion reducing power (FRAP) and Phosphomolybedenum complex (PMC) assays. The interactions between the total phenolics and flavonoids of the selected plants and virulent target proteins of the pathogenic bacteria were identified, characterized, and examined using Molecular Operating Environment (MOE) software. The fresh leaves of P. roxburghii, exhibited the highest total phenolic (744.6 ± 27.0 mg GAE/100 g) and carotenoid content (21.4 ± 0.4 mg/g) that was significantly varied from other samples. The EO of P. wallichiana, showed the highest total flavonoid content. In P. roxburghii and P. wallichiana, the fresh leaves exhibited the highest percentage of scavenging potential against the DPPH radical. Additionally, the EO from P. roxburghii and P. wallichiana have significant potential to reduce ferric ions, and molybdate ions, respectively) with significant differences. The molecular docking showed a significant interaction of compounds present in these pines leaves with Exotoxin A and Elastase B proteins. The essential oil (EO), hydrosol (HS), and crude extract derived from P. roxburghii and P. wallichiana could be exploited as natural sources of antioxidants, anti-inflammatory agents, and innovative medications against the human pathogen, Pseudomonas aeruginosa. |
| format | Article |
| id | doaj-art-0fa72bd191b54177aa9e12d3fc26b057 |
| institution | OA Journals |
| issn | 2331-1932 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cogent Food & Agriculture |
| spelling | doaj-art-0fa72bd191b54177aa9e12d3fc26b0572025-08-20T01:58:52ZengTaylor & Francis GroupCogent Food & Agriculture2331-19322024-12-0110110.1080/23311932.2024.2403648Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analysesSaba Mehmood0Arshad Mehmood Abbasi1Hazrat Hussain2Sabaz Ali Khan3Hayfa Habes Almutairi4Ahmed Mahmoud Ismail5Mohamed S. Al Saikhan6Hossam S. El-Beltagi7Department of Biotechnology, COMSATS University Islamabad-Abbottabad Campus, Abbottabad, PakistanDepartment of Environmental Sciences, COMSATS University Islamabad-Abbottabad Campus, Abbottabad, PakistanDepartment of Biotechnology, COMSATS University Islamabad-Abbottabad Campus, Abbottabad, PakistanDepartment of Biotechnology, COMSATS University Islamabad-Abbottabad Campus, Abbottabad, PakistanDepartment of Chemistry, College of Science, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaAgricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi ArabiaPinus roxburghii and Pinus wallichiana are traditionally plants used for various medicinal purposes worldwide. The present study focused on evaluating the phytochemical compounds and the free radical scavenging potential in various parts of P. roxburghii and P. wallichiana through in vitro and in silico study. Crude extract of the fresh leaves was obtained using methanol while steam distillation method was used for the extraction of the essential oil. The phytochemicals were determined by standard methods. Antioxidant Potential of the phytochemicals was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), Hydroxyl radical (OH) inhibition, Ferric ion reducing power (FRAP) and Phosphomolybedenum complex (PMC) assays. The interactions between the total phenolics and flavonoids of the selected plants and virulent target proteins of the pathogenic bacteria were identified, characterized, and examined using Molecular Operating Environment (MOE) software. The fresh leaves of P. roxburghii, exhibited the highest total phenolic (744.6 ± 27.0 mg GAE/100 g) and carotenoid content (21.4 ± 0.4 mg/g) that was significantly varied from other samples. The EO of P. wallichiana, showed the highest total flavonoid content. In P. roxburghii and P. wallichiana, the fresh leaves exhibited the highest percentage of scavenging potential against the DPPH radical. Additionally, the EO from P. roxburghii and P. wallichiana have significant potential to reduce ferric ions, and molybdate ions, respectively) with significant differences. The molecular docking showed a significant interaction of compounds present in these pines leaves with Exotoxin A and Elastase B proteins. The essential oil (EO), hydrosol (HS), and crude extract derived from P. roxburghii and P. wallichiana could be exploited as natural sources of antioxidants, anti-inflammatory agents, and innovative medications against the human pathogen, Pseudomonas aeruginosa.https://www.tandfonline.com/doi/10.1080/23311932.2024.2403648Antioxidant assaysessential oilflavonoidsmedicinal plantspineP. roxburghii |
| spellingShingle | Saba Mehmood Arshad Mehmood Abbasi Hazrat Hussain Sabaz Ali Khan Hayfa Habes Almutairi Ahmed Mahmoud Ismail Mohamed S. Al Saikhan Hossam S. El-Beltagi Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analyses Cogent Food & Agriculture Antioxidant assays essential oil flavonoids medicinal plants pine P. roxburghii |
| title | Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analyses |
| title_full | Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analyses |
| title_fullStr | Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analyses |
| title_full_unstemmed | Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analyses |
| title_short | Phytochemical profile, antioxidant and antibacterial activities analysis of crude extract and essential oil of Pinus roxburghii and Pinus wallichiana: in vitro and in silico analyses |
| title_sort | phytochemical profile antioxidant and antibacterial activities analysis of crude extract and essential oil of pinus roxburghii and pinus wallichiana in vitro and in silico analyses |
| topic | Antioxidant assays essential oil flavonoids medicinal plants pine P. roxburghii |
| url | https://www.tandfonline.com/doi/10.1080/23311932.2024.2403648 |
| work_keys_str_mv | AT sabamehmood phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses AT arshadmehmoodabbasi phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses AT hazrathussain phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses AT sabazalikhan phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses AT hayfahabesalmutairi phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses AT ahmedmahmoudismail phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses AT mohamedsalsaikhan phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses AT hossamselbeltagi phytochemicalprofileantioxidantandantibacterialactivitiesanalysisofcrudeextractandessentialoilofpinusroxburghiiandpinuswallichianainvitroandinsilicoanalyses |