Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways

Non-coding RNAs play an important role in the pathogenesis of prostate cancer (PC). This study aims to characterize the role of GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms in the pathogenesis of PC. Both INS allele of GAS5 rs145204276 and A allele of HOTAIR rs4759314 were identified to incre...

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Main Authors: Zhi-Hai Deng, Gan-Shen Yu, Bin Pan, Zhen-Hua Feng, Qiang Huang, Jian-Zhong Deng, Bo Chen, Shi-Kun Yang
Format: Article
Language:English
Published: Taylor & Francis Group 2020-01-01
Series:Artificial Cells, Nanomedicine, and Biotechnology
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Online Access:https://www.tandfonline.com/doi/10.1080/21691401.2019.1709859
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author Zhi-Hai Deng
Gan-Shen Yu
Bin Pan
Zhen-Hua Feng
Qiang Huang
Jian-Zhong Deng
Bo Chen
Shi-Kun Yang
author_facet Zhi-Hai Deng
Gan-Shen Yu
Bin Pan
Zhen-Hua Feng
Qiang Huang
Jian-Zhong Deng
Bo Chen
Shi-Kun Yang
author_sort Zhi-Hai Deng
collection DOAJ
description Non-coding RNAs play an important role in the pathogenesis of prostate cancer (PC). This study aims to characterize the role of GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms in the pathogenesis of PC. Both INS allele of GAS5 rs145204276 and A allele of HOTAIR rs4759314 were identified to increase the survival of PC patients. And patients carrying DEL/DEL + AG genotypes tend to present higher levels of HMGB1, GAS5, HOTAIR and lower levels of miR-1284 and miR-22. In addition, the transcription activity of GAS5 promoter was increased by the deletion allele of rs145204276 polymorphism, while the G allele of rs4759314 polymorphism increased the transcription activity of HOTAIR promoter. GAS5 and HOTAIR could bind to miR-1284 and miR-22, respectively, while miR-1284 and miR-22 could bind to the 3′UTR of HMGB1. Compared with the control group, the expressions of miR-1284 or miR-22 were decreased with the presence of GAS5 or HOTAIR, and the expression of HMGB1 was the highest in the GAS5 + HOTAIR group. In summary, the findings of this study demonstrated that both GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms could affect the prognosis of PC by modulating the expression of HMGB1 via modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways.
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series Artificial Cells, Nanomedicine, and Biotechnology
spelling doaj-art-0fa4c989a7fc451fbb9b9ec1f25f36782025-08-20T03:41:51ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-0148143544210.1080/21691401.2019.1709859Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathwaysZhi-Hai Deng0Gan-Shen Yu1Bin Pan2Zhen-Hua Feng3Qiang Huang4Jian-Zhong Deng5Bo Chen6Shi-Kun Yang7Department of Urology, Gaozhou People's Hospital, Gaozhou, Guangdong, ChinaDepartment of Urology, Maoming People's Hospital, Maoming, Guangdong, ChinaDepartment of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, ChinaDepartment of Urology, Gaozhou People's Hospital, Gaozhou, Guangdong, ChinaDepartment of Urology, Gaozhou People's Hospital, Gaozhou, Guangdong, ChinaDepartment of Urology, Gaozhou People's Hospital, Gaozhou, Guangdong, ChinaDepartment of Urology, Gaozhou People's Hospital, Gaozhou, Guangdong, ChinaDepartment of Urology, Yanghe People's hospital, Suqian, Jiangsu, ChinaNon-coding RNAs play an important role in the pathogenesis of prostate cancer (PC). This study aims to characterize the role of GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms in the pathogenesis of PC. Both INS allele of GAS5 rs145204276 and A allele of HOTAIR rs4759314 were identified to increase the survival of PC patients. And patients carrying DEL/DEL + AG genotypes tend to present higher levels of HMGB1, GAS5, HOTAIR and lower levels of miR-1284 and miR-22. In addition, the transcription activity of GAS5 promoter was increased by the deletion allele of rs145204276 polymorphism, while the G allele of rs4759314 polymorphism increased the transcription activity of HOTAIR promoter. GAS5 and HOTAIR could bind to miR-1284 and miR-22, respectively, while miR-1284 and miR-22 could bind to the 3′UTR of HMGB1. Compared with the control group, the expressions of miR-1284 or miR-22 were decreased with the presence of GAS5 or HOTAIR, and the expression of HMGB1 was the highest in the GAS5 + HOTAIR group. In summary, the findings of this study demonstrated that both GAS5 rs145204276 and HOTAIR rs4759314 polymorphisms could affect the prognosis of PC by modulating the expression of HMGB1 via modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways.https://www.tandfonline.com/doi/10.1080/21691401.2019.1709859Prostate cancerrs145204276rs4759314miR-1284miR-22GAS5
spellingShingle Zhi-Hai Deng
Gan-Shen Yu
Bin Pan
Zhen-Hua Feng
Qiang Huang
Jian-Zhong Deng
Bo Chen
Shi-Kun Yang
Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways
Artificial Cells, Nanomedicine, and Biotechnology
Prostate cancer
rs145204276
rs4759314
miR-1284
miR-22
GAS5
title Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways
title_full Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways
title_fullStr Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways
title_full_unstemmed Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways
title_short Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways
title_sort rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the gas5 mir 1284 hmgb1 and hotair mir 22 hmgb1 signalling pathways
topic Prostate cancer
rs145204276
rs4759314
miR-1284
miR-22
GAS5
url https://www.tandfonline.com/doi/10.1080/21691401.2019.1709859
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