Candida auris cells form giant lipid droplets to survive in harsh environments

Abstract The emerging fungal pathogen Candida auris is notorious for environmental persistence, which is a major contributor to outbreaks in healthcare settings. Here we report that giant lipid droplets (gLDs) inside C. auris cells play critical roles in the ability to survive harsh environments. C....

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Main Authors: Qiushi Zheng, Chao Li, Tianren Hu, Jian Bing, Clarissa J. Nobile, Haiqing Chu, Guanghua Huang
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08204-7
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author Qiushi Zheng
Chao Li
Tianren Hu
Jian Bing
Clarissa J. Nobile
Haiqing Chu
Guanghua Huang
author_facet Qiushi Zheng
Chao Li
Tianren Hu
Jian Bing
Clarissa J. Nobile
Haiqing Chu
Guanghua Huang
author_sort Qiushi Zheng
collection DOAJ
description Abstract The emerging fungal pathogen Candida auris is notorious for environmental persistence, which is a major contributor to outbreaks in healthcare settings. Here we report that giant lipid droplets (gLDs) inside C. auris cells play critical roles in the ability to survive harsh environments. C. auris cells that contain gLDs exhibit an increased tolerance to environmental stresses, antifungals, and host-associated antimicrobial peptides. These cells often undergo significant cell wall remodeling and sloughing of the outer layer of the cell wall. Lipidomics analysis indicates that cells with gLDs contain a significantly higher level of triacylglycerols, sterol esters, and other lipids, such as glycerolipids, sphingolipids, and sterol lipids. At the transcriptional level, a large set of differentially expressed genes was observed between C. auris cells with and without gLDs. Our study reveals that gLDs are a new strategy used by C. auris to adapt to stressful conditions and to persist in hospital environments.
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issn 2399-3642
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series Communications Biology
spelling doaj-art-0fa416895e7541f08fedfd2a7669f7de2025-08-20T02:34:19ZengNature PortfolioCommunications Biology2399-36422025-05-018111410.1038/s42003-025-08204-7Candida auris cells form giant lipid droplets to survive in harsh environmentsQiushi Zheng0Chao Li1Tianren Hu2Jian Bing3Clarissa J. Nobile4Haiqing Chu5Guanghua Huang6State Key Laboratory of Genetic Engineering, School of Life Sciences, Shanghai Institute of Infectious Disease and Biosecurity, and Department of infectious diseases, Huashan Hospital, Fudan UniversityState Key Laboratory of Genetic Engineering, School of Life Sciences, Shanghai Institute of Infectious Disease and Biosecurity, and Department of infectious diseases, Huashan Hospital, Fudan UniversityDepartment of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji UniversityState Key Laboratory of Genetic Engineering, School of Life Sciences, Shanghai Institute of Infectious Disease and Biosecurity, and Department of infectious diseases, Huashan Hospital, Fudan UniversityDepartment of Molecular and Cell Biology, University of CaliforniaDepartment of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji UniversityState Key Laboratory of Genetic Engineering, School of Life Sciences, Shanghai Institute of Infectious Disease and Biosecurity, and Department of infectious diseases, Huashan Hospital, Fudan UniversityAbstract The emerging fungal pathogen Candida auris is notorious for environmental persistence, which is a major contributor to outbreaks in healthcare settings. Here we report that giant lipid droplets (gLDs) inside C. auris cells play critical roles in the ability to survive harsh environments. C. auris cells that contain gLDs exhibit an increased tolerance to environmental stresses, antifungals, and host-associated antimicrobial peptides. These cells often undergo significant cell wall remodeling and sloughing of the outer layer of the cell wall. Lipidomics analysis indicates that cells with gLDs contain a significantly higher level of triacylglycerols, sterol esters, and other lipids, such as glycerolipids, sphingolipids, and sterol lipids. At the transcriptional level, a large set of differentially expressed genes was observed between C. auris cells with and without gLDs. Our study reveals that gLDs are a new strategy used by C. auris to adapt to stressful conditions and to persist in hospital environments.https://doi.org/10.1038/s42003-025-08204-7
spellingShingle Qiushi Zheng
Chao Li
Tianren Hu
Jian Bing
Clarissa J. Nobile
Haiqing Chu
Guanghua Huang
Candida auris cells form giant lipid droplets to survive in harsh environments
Communications Biology
title Candida auris cells form giant lipid droplets to survive in harsh environments
title_full Candida auris cells form giant lipid droplets to survive in harsh environments
title_fullStr Candida auris cells form giant lipid droplets to survive in harsh environments
title_full_unstemmed Candida auris cells form giant lipid droplets to survive in harsh environments
title_short Candida auris cells form giant lipid droplets to survive in harsh environments
title_sort candida auris cells form giant lipid droplets to survive in harsh environments
url https://doi.org/10.1038/s42003-025-08204-7
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