Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.

<h4>Background</h4>Neisseria meningitidis serogroup A is the main causative pathogen of meningitis epidemics in sub-Saharan Africa. In recent years, serogroup W135 has also been the cause of epidemics. Mass vaccination campaigns with polysaccharide vaccines are key elements in controllin...

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Main Authors: Philippe J Guerin, Lisbeth M Naess, Carole Fogg, Einar Rosenqvist, Loretxu Pinoges, Francis Bajunirwe, Carolyn Nabasumba, Ray Borrow, Leif O Frøholm, Salah Ghabri, Vincent Batwala, Rogers Twesigye, Ingeborg S Aaberge, John-Arne Røttingen, Patrice Piola, Dominique A Caugant
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS Neglected Tropical Diseases
Online Access:https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000342&type=printable
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author Philippe J Guerin
Lisbeth M Naess
Carole Fogg
Einar Rosenqvist
Loretxu Pinoges
Francis Bajunirwe
Carolyn Nabasumba
Ray Borrow
Leif O Frøholm
Salah Ghabri
Vincent Batwala
Rogers Twesigye
Ingeborg S Aaberge
John-Arne Røttingen
Patrice Piola
Dominique A Caugant
author_facet Philippe J Guerin
Lisbeth M Naess
Carole Fogg
Einar Rosenqvist
Loretxu Pinoges
Francis Bajunirwe
Carolyn Nabasumba
Ray Borrow
Leif O Frøholm
Salah Ghabri
Vincent Batwala
Rogers Twesigye
Ingeborg S Aaberge
John-Arne Røttingen
Patrice Piola
Dominique A Caugant
author_sort Philippe J Guerin
collection DOAJ
description <h4>Background</h4>Neisseria meningitidis serogroup A is the main causative pathogen of meningitis epidemics in sub-Saharan Africa. In recent years, serogroup W135 has also been the cause of epidemics. Mass vaccination campaigns with polysaccharide vaccines are key elements in controlling these epidemics. Facing global vaccine shortage, we explored the use of fractional doses of a licensed A/C/Y/W135 polysaccharide meningococcal vaccine.<h4>Methods and findings</h4>We conducted a randomized, non-inferiority trial in 750 healthy volunteers 2-19 years old in Mbarara, Uganda, to compare the immune response of the full dose of the vaccine versus fractional doses (1/5 or 1/10). Safety and tolerability data were collected for all subjects during the 4 weeks following the injection. Pre- and post-vaccination sera were analyzed by measuring serum bactericidal activity (SBA) with baby rabbit complement. A responder was defined as a subject with a > or =4-fold increase in SBA against a target strain from each serogroup and SBA titer > or =128. For serogroup W135, 94% and 97% of the vaccinees in the 1/5- and 1/10-dose arms, respectively, were responders, versus 94% in the full-dose arm; for serogroup A, 92% and 88% were responders, respectively, versus 95%. Non-inferiority was demonstrated between the full dose and both fractional doses in SBA seroresponse against serogroups W135 and Y, in total population analysis. Non-inferiority was shown between the full and 1/5 doses for serogroup A in the population non-immune prior to vaccination. Non-inferiority was not shown for any of the fractionate doses for serogroup C. Safety and tolerability data were favourable, as observed in other studies.<h4>Conclusions</h4>While the advent of conjugate A vaccine is anticipated to largely contribute to control serogroup A outbreaks in Africa, the scale-up of its production will not cover the entire "Meningitis Belt" target population for at least the next 3 to 5 years. In view of the current shortage of meningococcal vaccines for Africa, the use of 1/5 fractional doses should be considered as an alternative in mass vaccination campaigns.<h4>Trial registration</h4>ClinicalTrials.gov NCT00271479.
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spelling doaj-art-0fa0f81898a442ac9570fce2e9b11dff2025-08-20T02:20:09ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352008-01-01212e34210.1371/journal.pntd.0000342Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.Philippe J GuerinLisbeth M NaessCarole FoggEinar RosenqvistLoretxu PinogesFrancis BajunirweCarolyn NabasumbaRay BorrowLeif O FrøholmSalah GhabriVincent BatwalaRogers TwesigyeIngeborg S AabergeJohn-Arne RøttingenPatrice PiolaDominique A Caugant<h4>Background</h4>Neisseria meningitidis serogroup A is the main causative pathogen of meningitis epidemics in sub-Saharan Africa. In recent years, serogroup W135 has also been the cause of epidemics. Mass vaccination campaigns with polysaccharide vaccines are key elements in controlling these epidemics. Facing global vaccine shortage, we explored the use of fractional doses of a licensed A/C/Y/W135 polysaccharide meningococcal vaccine.<h4>Methods and findings</h4>We conducted a randomized, non-inferiority trial in 750 healthy volunteers 2-19 years old in Mbarara, Uganda, to compare the immune response of the full dose of the vaccine versus fractional doses (1/5 or 1/10). Safety and tolerability data were collected for all subjects during the 4 weeks following the injection. Pre- and post-vaccination sera were analyzed by measuring serum bactericidal activity (SBA) with baby rabbit complement. A responder was defined as a subject with a > or =4-fold increase in SBA against a target strain from each serogroup and SBA titer > or =128. For serogroup W135, 94% and 97% of the vaccinees in the 1/5- and 1/10-dose arms, respectively, were responders, versus 94% in the full-dose arm; for serogroup A, 92% and 88% were responders, respectively, versus 95%. Non-inferiority was demonstrated between the full dose and both fractional doses in SBA seroresponse against serogroups W135 and Y, in total population analysis. Non-inferiority was shown between the full and 1/5 doses for serogroup A in the population non-immune prior to vaccination. Non-inferiority was not shown for any of the fractionate doses for serogroup C. Safety and tolerability data were favourable, as observed in other studies.<h4>Conclusions</h4>While the advent of conjugate A vaccine is anticipated to largely contribute to control serogroup A outbreaks in Africa, the scale-up of its production will not cover the entire "Meningitis Belt" target population for at least the next 3 to 5 years. In view of the current shortage of meningococcal vaccines for Africa, the use of 1/5 fractional doses should be considered as an alternative in mass vaccination campaigns.<h4>Trial registration</h4>ClinicalTrials.gov NCT00271479.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000342&type=printable
spellingShingle Philippe J Guerin
Lisbeth M Naess
Carole Fogg
Einar Rosenqvist
Loretxu Pinoges
Francis Bajunirwe
Carolyn Nabasumba
Ray Borrow
Leif O Frøholm
Salah Ghabri
Vincent Batwala
Rogers Twesigye
Ingeborg S Aaberge
John-Arne Røttingen
Patrice Piola
Dominique A Caugant
Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.
PLoS Neglected Tropical Diseases
title Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.
title_full Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.
title_fullStr Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.
title_full_unstemmed Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.
title_short Immunogenicity of fractional doses of tetravalent a/c/y/w135 meningococcal polysaccharide vaccine: results from a randomized non-inferiority controlled trial in Uganda.
title_sort immunogenicity of fractional doses of tetravalent a c y w135 meningococcal polysaccharide vaccine results from a randomized non inferiority controlled trial in uganda
url https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000342&type=printable
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