Fungal Warriors: Effects of <i>Beauveria bassiana</i> and <i>Purpureocillium lilacinum</i> on CCYV-Carrying Whiteflies

<i>Bemisia tabaci</i> is a major agricultural pest that affects both greenhouse and field crops by feeding on plant sap, which impairs plant growth, and by secreting honeydew, promotes sooty mold growth that further reduces photosynthesis. Additionally, these insects are vectors for viru...

Full description

Saved in:
Bibliographic Details
Main Authors: Dan Zhai, Hang Lu, Suyao Liu, Jialei Liu, Wanyu Zhang, Jingjing Wu, Jingjing Li, Rune Bai, Fengming Yan, Chenchen Zhao
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Biomolecules
Subjects:
Online Access:https://www.mdpi.com/2218-273X/15/4/593
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<i>Bemisia tabaci</i> is a major agricultural pest that affects both greenhouse and field crops by feeding on plant sap, which impairs plant growth, and by secreting honeydew, promotes sooty mold growth that further reduces photosynthesis. Additionally, these insects are vectors for viruses such as the cucurbit chlorotic yellows virus (CCYV), which causes significant damage to cucurbit crops. Traditional chemical pesticide treatments have limitations, including the development of resistance, harm to non-target organisms, and environmental contamination. Traditional chemical pesticides have limitations when it comes to controlling plants infested by CCYV and whitefly. However, the underlying reasons for these limitations remain unclear, as does the impact of entomopathogenic fungi on whitefly responses. This study explores the potential of using biological control agents, specifically <i>Beauveria bassiana</i> and <i>Purpureocillium lilacinum</i>, to manage whitefly populations and control CCYV transmission. Laboratory experiments were conducted to evaluate the pathogenicity of these fungi on non/viruliferous whitefly. The results indicated that both fungi effectively reduced whitefly populations, with <i>B. bassiana</i> showing particularly strong adverse effects. Whiteflies infected with CCYV exhibited a higher LC<sub>50</sub> to <i>B. bassiana</i> and <i>P. lilacinum</i>. Furthermore, bio-pesticides significantly altered the bacterial microbiome dynamics of the whitefly. Interestingly, CCYV increased the susceptibility of whiteflies to entomopathogenic fungus. The findings suggest that these biocontrol agents offer a sustainable alternative to chemical pesticides. Our study unraveled a new horizon for the multiple interaction theories among bio-pesticides–insects–symbionts–viruses.
ISSN:2218-273X