A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana roots
Abstract Objective Plants control the direction of root growth toward moisture due to their sessile nature, a phenomenon known as hydrotropism. In Arabidopsis thaliana, the MIZU-KUSSEI1 (MIZ1) protein is essential for the hydrotropic response. To investigate the molecular mechanism of hydrotropism i...
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BMC
2025-07-01
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| Online Access: | https://doi.org/10.1186/s13104-025-07345-6 |
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| author | Su-Hwan Kwak Wonkeun John Park |
| author_facet | Su-Hwan Kwak Wonkeun John Park |
| author_sort | Su-Hwan Kwak |
| collection | DOAJ |
| description | Abstract Objective Plants control the direction of root growth toward moisture due to their sessile nature, a phenomenon known as hydrotropism. In Arabidopsis thaliana, the MIZU-KUSSEI1 (MIZ1) protein is essential for the hydrotropic response. To investigate the molecular mechanism of hydrotropism in cotton, we examined the function of GhMIZ1, the closest homolog of Arabidopsis thaliana MIZ1. Results The GhMIZ1 gene, amplified from Gossypium hirsutum genomic DNA, was introduced into the Arabidopsis thaliana miz1 null mutant genome. Hydrotropism response in the transgenic mutant roots overexpressing GhMIZ1 was tested by using split-agarose plates containing 20% sorbitol. The expression of GhMIZ1 partially restored the hydrotropic response in miz1 mutant roots. This finding suggests that GhMIZ1 is capable of mediating hydrotropism in Arabidopsis thaliana, indicating that it serves as a functional homolog of MIZU-KUSSEI1. |
| format | Article |
| id | doaj-art-0f9933f19efe43848010eabde5d2397f |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
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| series | BMC Research Notes |
| spelling | doaj-art-0f9933f19efe43848010eabde5d2397f2025-08-20T03:42:47ZengBMCBMC Research Notes1756-05002025-07-011811710.1186/s13104-025-07345-6A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana rootsSu-Hwan Kwak0Wonkeun John Park1Department of Natural and Life Sciences, Long Island UniversityCrop Diversity & Improvement Laboratory, Pee Dee Research & Education Center, Clemson UniversityAbstract Objective Plants control the direction of root growth toward moisture due to their sessile nature, a phenomenon known as hydrotropism. In Arabidopsis thaliana, the MIZU-KUSSEI1 (MIZ1) protein is essential for the hydrotropic response. To investigate the molecular mechanism of hydrotropism in cotton, we examined the function of GhMIZ1, the closest homolog of Arabidopsis thaliana MIZ1. Results The GhMIZ1 gene, amplified from Gossypium hirsutum genomic DNA, was introduced into the Arabidopsis thaliana miz1 null mutant genome. Hydrotropism response in the transgenic mutant roots overexpressing GhMIZ1 was tested by using split-agarose plates containing 20% sorbitol. The expression of GhMIZ1 partially restored the hydrotropic response in miz1 mutant roots. This finding suggests that GhMIZ1 is capable of mediating hydrotropism in Arabidopsis thaliana, indicating that it serves as a functional homolog of MIZU-KUSSEI1.https://doi.org/10.1186/s13104-025-07345-6Arabidopsis thalianaCottonGossypium hirsutumHydrotropismMIZU-KUSSEI1 |
| spellingShingle | Su-Hwan Kwak Wonkeun John Park A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana roots BMC Research Notes Arabidopsis thaliana Cotton Gossypium hirsutum Hydrotropism MIZU-KUSSEI1 |
| title | A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana roots |
| title_full | A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana roots |
| title_fullStr | A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana roots |
| title_full_unstemmed | A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana roots |
| title_short | A functional complementation of a cotton MIZU-KUSSEI1 homolog in the hydrotropism response of Arabidopsis thaliana roots |
| title_sort | functional complementation of a cotton mizu kussei1 homolog in the hydrotropism response of arabidopsis thaliana roots |
| topic | Arabidopsis thaliana Cotton Gossypium hirsutum Hydrotropism MIZU-KUSSEI1 |
| url | https://doi.org/10.1186/s13104-025-07345-6 |
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