Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120

Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120

The difficulty and expense of preparing protein samples highly enriched in stable isotopes is a bottleneck for structural studies by nuclear magnetic resonance (NMR) spectroscopy. We have developed a new regulatable expression/labeling vector system in the cyanobacterium Anabaena sp. PCC 7120 using...

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Bibliographic Details
Main Authors: Dominique Desplancq, Corinne Bernard, Annie-Paule Sibler, Bruno Kieffer, Laurent Miguet, Noëlle Potier, Alain Van Dorsselaer, Etienne Weiss
Format: Article
Language:English
Published: Taylor & Francis Group 2005-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/05393RR02
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