Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infection
An expansion of plasma anelloviruses and dysregulation of inflammation was associated with HIV-1 infection. However, how antiretroviral therapy (ART) affects the dynamics of plasma virome and cytokine profile remains largely unknown. To characterize the dynamics of plasma virome and cytokines in HIV...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2467168 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849719020834521088 |
|---|---|
| author | Yingying Ma Min Zhang Zhenyan Wang Le Cao Yanpeng Li Zhenzhou Wan Yakhouba Kane Gang Wang Xin Li Chiyu Zhang |
| author_facet | Yingying Ma Min Zhang Zhenyan Wang Le Cao Yanpeng Li Zhenzhou Wan Yakhouba Kane Gang Wang Xin Li Chiyu Zhang |
| author_sort | Yingying Ma |
| collection | DOAJ |
| description | An expansion of plasma anelloviruses and dysregulation of inflammation was associated with HIV-1 infection. However, how antiretroviral therapy (ART) affects the dynamics of plasma virome and cytokine profile remains largely unknown. To characterize the dynamics of plasma virome and cytokines in HIV-1-infected individuals before and during the first year of ART, a cohort of 26 HIV-1-infected individuals and 19 healthy controls was recruited. Blood samples were collected and subjected to metagenomic analysis and the measurement of 27 cytokines. Metagenomic analysis revealed an increased abundance and prevalence of human pegivirus type 1 (HPgV-1) and a slightly decreased diversity and abundance of anellovirus in plasma of HIV-1-infected individuals after ART. No obvious impact was observed on other plasma commensal viruses. Increased abundance and prevalence of HPgV-1 were further confirmed by RT-qPCR assay in a larger cohort of 114 HIV-1-infected individuals. Notably, most dysregulated cytokines were not fully restored by ART, with extremely abnormal levels of IL-10, GM-CSF, VEGF, and eotaxin, and a significantly increased level of plasma I-FABP. Anelloviruses showed significantly negative correlations with other commensal viruses except HPgV-1 but had positive correlations with several anti-inflammatory and Th1 cytokines. These results suggest that short-term ART may not significantly correct the virome and cytokine dysregulations induced by HIV-1 infection. The results highlight a need for further investigation into the long-term effects of ART on virome and cytokine profiles in HIV-1-infected individuals. |
| format | Article |
| id | doaj-art-0f84bfcedea2429281c5b32feb2d1c8e |
| institution | DOAJ |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-0f84bfcedea2429281c5b32feb2d1c8e2025-08-20T03:12:14ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2467168Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infectionYingying Ma0Min Zhang1Zhenyan Wang2Le Cao3Yanpeng Li4Zhenzhou Wan5Yakhouba Kane6Gang Wang7Xin Li8Chiyu Zhang9Shanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaMedical Laboratory,Taizhou Fourth People’s Hospital, Taizhou, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaShanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaAn expansion of plasma anelloviruses and dysregulation of inflammation was associated with HIV-1 infection. However, how antiretroviral therapy (ART) affects the dynamics of plasma virome and cytokine profile remains largely unknown. To characterize the dynamics of plasma virome and cytokines in HIV-1-infected individuals before and during the first year of ART, a cohort of 26 HIV-1-infected individuals and 19 healthy controls was recruited. Blood samples were collected and subjected to metagenomic analysis and the measurement of 27 cytokines. Metagenomic analysis revealed an increased abundance and prevalence of human pegivirus type 1 (HPgV-1) and a slightly decreased diversity and abundance of anellovirus in plasma of HIV-1-infected individuals after ART. No obvious impact was observed on other plasma commensal viruses. Increased abundance and prevalence of HPgV-1 were further confirmed by RT-qPCR assay in a larger cohort of 114 HIV-1-infected individuals. Notably, most dysregulated cytokines were not fully restored by ART, with extremely abnormal levels of IL-10, GM-CSF, VEGF, and eotaxin, and a significantly increased level of plasma I-FABP. Anelloviruses showed significantly negative correlations with other commensal viruses except HPgV-1 but had positive correlations with several anti-inflammatory and Th1 cytokines. These results suggest that short-term ART may not significantly correct the virome and cytokine dysregulations induced by HIV-1 infection. The results highlight a need for further investigation into the long-term effects of ART on virome and cytokine profiles in HIV-1-infected individuals.https://www.tandfonline.com/doi/10.1080/21505594.2025.2467168ViromeHIV-1ARTanellovirushuman pegivirus type 1cytokines |
| spellingShingle | Yingying Ma Min Zhang Zhenyan Wang Le Cao Yanpeng Li Zhenzhou Wan Yakhouba Kane Gang Wang Xin Li Chiyu Zhang Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infection Virulence Virome HIV-1 ART anellovirus human pegivirus type 1 cytokines |
| title | Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infection |
| title_full | Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infection |
| title_fullStr | Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infection |
| title_full_unstemmed | Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infection |
| title_short | Short-term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by HIV-1 infection |
| title_sort | short term antiretroviral therapy may not correct the dysregulations of plasma virome and cytokines induced by hiv 1 infection |
| topic | Virome HIV-1 ART anellovirus human pegivirus type 1 cytokines |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2467168 |
| work_keys_str_mv | AT yingyingma shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT minzhang shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT zhenyanwang shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT lecao shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT yanpengli shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT zhenzhouwan shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT yakhoubakane shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT gangwang shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT xinli shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection AT chiyuzhang shorttermantiretroviraltherapymaynotcorrectthedysregulationsofplasmaviromeandcytokinesinducedbyhiv1infection |