Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China

This study identifies and characterizes pathogenic Fusarium species and their trichothecene genotypes in wheat crops grown in seven regions of Hebei Province, China, from 2019 to 2021. Species-specific primers confirmed the morphological identification of 689 Fusarium strains recovered from wheat se...

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Main Authors: Kahsay Tadesse Mawcha, Mewael Kiros Assefa, Yiying Xu, Na Zhang, Wenxiang Yang, Dennis Ndolo
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:NFS Journal
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352364625000197
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author Kahsay Tadesse Mawcha
Mewael Kiros Assefa
Yiying Xu
Na Zhang
Wenxiang Yang
Dennis Ndolo
author_facet Kahsay Tadesse Mawcha
Mewael Kiros Assefa
Yiying Xu
Na Zhang
Wenxiang Yang
Dennis Ndolo
author_sort Kahsay Tadesse Mawcha
collection DOAJ
description This study identifies and characterizes pathogenic Fusarium species and their trichothecene genotypes in wheat crops grown in seven regions of Hebei Province, China, from 2019 to 2021. Species-specific primers confirmed the morphological identification of 689 Fusarium strains recovered from wheat seedlings with symptomatic crown/sub-crown tissues. The results suggest that Fusarium pseudograminearum was the most detected strain, accounting for 91 % of the identified strains. Other species, including F. graminearum, F. oxysporum, F. proliferatum, F. asiaticum, and F. culmorum, were detected at lower rates, ranging from 0.15 % to 8.56 %. Toxin genotype detection results showed that F. pseudograminearum and F. graminearum were the most common species linked to wheat crown rot, producing high detection rates of trichothecene genotypes. F. pseudograminearum strains had detection rates of 84.50 % for deoxynivalenol (DON) and other mycotoxins, including 3-acetyl-deoxynivalenol (3-AcDON) and 15-acetyl-deoxynivalenol (15-AcDON). On the other hand, strains (F. graminearum) of the 15-AcDON chemotype were detected at 15 L-AcDON, with detection rates of 34.37 %, 21.88 %, 21.88 %, and 18.75 % in Handan, Shijiazhuang, Xingtai, and Baoding, respectively. The study also indicated that F. pseudograminearum and F. graminearum had strong pathogenicity, while F. asiaticum and F. culmorum showed weak pathogenicity to wheat in Hebei Province. This study identifies F. pseudograminearum as the predominant cause of Fusarium crown rot in Hebei Province, with the majority of strains classified as DON genotypes.
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issn 2352-3646
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spelling doaj-art-0f81e32e5ff54f359bb6d4379e37bd512025-08-20T03:25:12ZengElsevierNFS Journal2352-36462025-08-013910023010.1016/j.nfs.2025.100230Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, ChinaKahsay Tadesse Mawcha0Mewael Kiros Assefa1Yiying Xu2Na Zhang3Wenxiang Yang4Dennis Ndolo5College of Plant Protection, Hebei Agricultural University, 289 Lingyusi Street, Baoding, Hebei 071001, China; Department of Plant Sciences, Aksum University Shire Campus, Shire, Tigray, Ethiopia; Biopesticides Group, International Centre for Genetic Engineering and Biotechnology, Cape Town, South AfricaDepartment of Plant Sciences, Aksum University Shire Campus, Shire, Tigray, Ethiopia; Plant Physiology Sector Department of Biology, Federal University of Lavras-UFLA, BrazilCollege of Plant Protection, Hebei Agricultural University, 289 Lingyusi Street, Baoding, Hebei 071001, ChinaCollege of Plant Protection, Hebei Agricultural University, 289 Lingyusi Street, Baoding, Hebei 071001, China; Corresponding authors.College of Plant Protection, Hebei Agricultural University, 289 Lingyusi Street, Baoding, Hebei 071001, China; Corresponding authors.Biopesticides Group, International Centre for Genetic Engineering and Biotechnology, Cape Town, South AfricaThis study identifies and characterizes pathogenic Fusarium species and their trichothecene genotypes in wheat crops grown in seven regions of Hebei Province, China, from 2019 to 2021. Species-specific primers confirmed the morphological identification of 689 Fusarium strains recovered from wheat seedlings with symptomatic crown/sub-crown tissues. The results suggest that Fusarium pseudograminearum was the most detected strain, accounting for 91 % of the identified strains. Other species, including F. graminearum, F. oxysporum, F. proliferatum, F. asiaticum, and F. culmorum, were detected at lower rates, ranging from 0.15 % to 8.56 %. Toxin genotype detection results showed that F. pseudograminearum and F. graminearum were the most common species linked to wheat crown rot, producing high detection rates of trichothecene genotypes. F. pseudograminearum strains had detection rates of 84.50 % for deoxynivalenol (DON) and other mycotoxins, including 3-acetyl-deoxynivalenol (3-AcDON) and 15-acetyl-deoxynivalenol (15-AcDON). On the other hand, strains (F. graminearum) of the 15-AcDON chemotype were detected at 15 L-AcDON, with detection rates of 34.37 %, 21.88 %, 21.88 %, and 18.75 % in Handan, Shijiazhuang, Xingtai, and Baoding, respectively. The study also indicated that F. pseudograminearum and F. graminearum had strong pathogenicity, while F. asiaticum and F. culmorum showed weak pathogenicity to wheat in Hebei Province. This study identifies F. pseudograminearum as the predominant cause of Fusarium crown rot in Hebei Province, with the majority of strains classified as DON genotypes.http://www.sciencedirect.com/science/article/pii/S2352364625000197ChemotypesFusarium speciesMycotoxinPathogenicityWheat crown rot
spellingShingle Kahsay Tadesse Mawcha
Mewael Kiros Assefa
Yiying Xu
Na Zhang
Wenxiang Yang
Dennis Ndolo
Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China
NFS Journal
Chemotypes
Fusarium species
Mycotoxin
Pathogenicity
Wheat crown rot
title Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China
title_full Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China
title_fullStr Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China
title_full_unstemmed Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China
title_short Characterization of Fusarium crown rot pathogens and trichothecene genotypes in wheat crops of Hebei province, China
title_sort characterization of fusarium crown rot pathogens and trichothecene genotypes in wheat crops of hebei province china
topic Chemotypes
Fusarium species
Mycotoxin
Pathogenicity
Wheat crown rot
url http://www.sciencedirect.com/science/article/pii/S2352364625000197
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