The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico Study
Pancreatic cancer affects many people and is quite aggressive. Metallic nanoparticles may be an alternative treatment for this disease. In this work, the antiproliferative activity of biosynthesized silver nanoparticles was evaluated. <i>Stenocereus queretaroensis</i> peel extract was us...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2304-6740/12/12/317 |
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| author | Angélica Sofía González-Garibay Alba Adriana Vallejo-Cardona Ariadna Abigail Villarreal-Amézquita Iván Moisés Sánchez-Hernández Omar Ricardo Torres-González Eduardo Padilla-Camberos |
| author_facet | Angélica Sofía González-Garibay Alba Adriana Vallejo-Cardona Ariadna Abigail Villarreal-Amézquita Iván Moisés Sánchez-Hernández Omar Ricardo Torres-González Eduardo Padilla-Camberos |
| author_sort | Angélica Sofía González-Garibay |
| collection | DOAJ |
| description | Pancreatic cancer affects many people and is quite aggressive. Metallic nanoparticles may be an alternative treatment for this disease. In this work, the antiproliferative activity of biosynthesized silver nanoparticles was evaluated. <i>Stenocereus queretaroensis</i> peel extract was used as a reducing agent for nanoparticle synthesis; the characterization was carried out using spectroscopic techniques, X-ray diffraction, and microscopy. The antiproliferative effect was evaluated in the MIA PaCa-2 pancreatic tumoral cell line, and a molecular docking test was run with the STAT3 protein. The results obtained show that it was possible to synthesize silver nanoparticles from the plant extract, which was confirmed through characterization studies. The average size of the nanoparticles was measured to be 48.8 nm, and they predominantly exhibited a spherical shape. The antiproliferative effect was demonstrated in the pancreatic cell line, with an IC<sub>50</sub> value of 15.66 µg/mL. The molecular docking analysis predicted a strong interaction with the STAT3 protein, with a binding energy value of −6.47 obtained. With these results, it is concluded that biosynthesized silver nanoparticles inhibit pancreatic tumor cell growth and may represent an innovative cancer treatment. |
| format | Article |
| id | doaj-art-0f7a2c6626284bdf9ba7defad8f7cbcd |
| institution | DOAJ |
| issn | 2304-6740 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Inorganics |
| spelling | doaj-art-0f7a2c6626284bdf9ba7defad8f7cbcd2025-08-20T02:53:37ZengMDPI AGInorganics2304-67402024-12-01121231710.3390/inorganics12120317The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico StudyAngélica Sofía González-Garibay0Alba Adriana Vallejo-Cardona1Ariadna Abigail Villarreal-Amézquita2Iván Moisés Sánchez-Hernández3Omar Ricardo Torres-González4Eduardo Padilla-Camberos5Medical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Av. Normalistas No. 800 Col. Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoMedical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Av. Normalistas No. 800 Col. Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoMedical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Av. Normalistas No. 800 Col. Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoMedical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Av. Normalistas No. 800 Col. Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoMedical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Av. Normalistas No. 800 Col. Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoMedical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco, A.C. (CIATEJ), Av. Normalistas No. 800 Col. Colinas de la Normal, Guadalajara C.P. 44270, Jalisco, MexicoPancreatic cancer affects many people and is quite aggressive. Metallic nanoparticles may be an alternative treatment for this disease. In this work, the antiproliferative activity of biosynthesized silver nanoparticles was evaluated. <i>Stenocereus queretaroensis</i> peel extract was used as a reducing agent for nanoparticle synthesis; the characterization was carried out using spectroscopic techniques, X-ray diffraction, and microscopy. The antiproliferative effect was evaluated in the MIA PaCa-2 pancreatic tumoral cell line, and a molecular docking test was run with the STAT3 protein. The results obtained show that it was possible to synthesize silver nanoparticles from the plant extract, which was confirmed through characterization studies. The average size of the nanoparticles was measured to be 48.8 nm, and they predominantly exhibited a spherical shape. The antiproliferative effect was demonstrated in the pancreatic cell line, with an IC<sub>50</sub> value of 15.66 µg/mL. The molecular docking analysis predicted a strong interaction with the STAT3 protein, with a binding energy value of −6.47 obtained. With these results, it is concluded that biosynthesized silver nanoparticles inhibit pancreatic tumor cell growth and may represent an innovative cancer treatment.https://www.mdpi.com/2304-6740/12/12/317silver nanoparticlesantiproliferative<i>Stenocereus queretaroensis</i> |
| spellingShingle | Angélica Sofía González-Garibay Alba Adriana Vallejo-Cardona Ariadna Abigail Villarreal-Amézquita Iván Moisés Sánchez-Hernández Omar Ricardo Torres-González Eduardo Padilla-Camberos The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico Study Inorganics silver nanoparticles antiproliferative <i>Stenocereus queretaroensis</i> |
| title | The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico Study |
| title_full | The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico Study |
| title_fullStr | The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico Study |
| title_full_unstemmed | The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico Study |
| title_short | The In Vitro Cytotoxic Potential of Biosynthesized Silver Nanoparticles in MIA PaCa-2 Cells Supported with an In Silico Study |
| title_sort | in vitro cytotoxic potential of biosynthesized silver nanoparticles in mia paca 2 cells supported with an in silico study |
| topic | silver nanoparticles antiproliferative <i>Stenocereus queretaroensis</i> |
| url | https://www.mdpi.com/2304-6740/12/12/317 |
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