IFITM3 affects the level of antibody response after influenza vaccination
Interferon-induced transmembrane protein 3 (IFITM3) as an antiviral factor can inhibit replication of several viruses including influenza virus. A single-nucleotide polymorphism rs12252-C of IFITM3 results in a truncated IFITM3 protein lacking its first 21 amino acids, which is much higher in the Ha...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1756696 |
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| _version_ | 1850203284367736832 |
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| author | Na Lei Yan Li Qiang Sun Jian Lu Jianfang Zhou Zi Li Liqi Liu Junfeng Guo Kun Qin Haibin Wang Jianhong Zhao Chong Li Lingli Sun Dayan Wang Zhendong Zhao Yuelong Shu |
| author_facet | Na Lei Yan Li Qiang Sun Jian Lu Jianfang Zhou Zi Li Liqi Liu Junfeng Guo Kun Qin Haibin Wang Jianhong Zhao Chong Li Lingli Sun Dayan Wang Zhendong Zhao Yuelong Shu |
| author_sort | Na Lei |
| collection | DOAJ |
| description | Interferon-induced transmembrane protein 3 (IFITM3) as an antiviral factor can inhibit replication of several viruses including influenza virus. A single-nucleotide polymorphism rs12252-C of IFITM3 results in a truncated IFITM3 protein lacking its first 21 amino acids, which is much higher in the Han Chinese population and associated with severe illness in adults infected with pandemic influenza H1N1/09 virus. To investigate if IFITM3 or IFITM3 rs12252-C could affect the antibody response after influenza vaccination, we detected the haemagglutination inhibition (HI) of 171 healthy young adult volunteers (IFITM3 rs12252-C/C, C/T, T/T carriers) and in an IFITM3-deletion mouse model (Ifitm3-/-) after trivalent inactivated vaccine (TIV) immunization. Seroconversion rates for H1N1, H3N2 and B viruses in IFITM3 rs12252-C/C genotype carriers was lower compared with C/T and T/T donors. Significantly lower levels of specific antibodies to H1N1, H3N2 and B viruses and total IgG were observed in Ifitm3-/- mice. Correspondingly, the numbers of splenic germinal centre (GC) B cells, plasma cells, TIV-specific IgG+ antibody secreting cells and T follicular helper cells in Ifitm3-/- mice were lower compared with wild type mice. However, the number of memory B cells was higher in Ifitm3-/- mice at day 7 after booster. The HI level of Ifitm3-/- mice remained lower than WT mice after third vaccination. Moreover, the transcriptional network regulating GC B cell and plasma cell differentiation was abnormal in Ifitm3-/- mice. Our results indicate that IFITM3 deletion attenuated the antibody response. The mechanism of influenza-IFITM3 interactions affecting the antibody response requires further investigation. |
| format | Article |
| id | doaj-art-0f6ca8302b06402eae67002cfd7eac62 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-0f6ca8302b06402eae67002cfd7eac622025-08-20T02:11:34ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019197698710.1080/22221751.2020.1756696IFITM3 affects the level of antibody response after influenza vaccinationNa Lei0Yan Li1Qiang Sun2Jian Lu3Jianfang Zhou4Zi Li5Liqi Liu6Junfeng Guo7Kun Qin8Haibin Wang9Jianhong Zhao10Chong Li11Lingli Sun12Dayan Wang13Zhendong Zhao14Yuelong Shu15National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaChaoyang District Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaChaoyang District Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaChaoyang District Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaChaoyang District Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaNHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of ChinaNational Institute for Viral Disease Control and Prevention, Chinese Center for Disease Prevention and Control, Beijing, People’s Republic of ChinaInterferon-induced transmembrane protein 3 (IFITM3) as an antiviral factor can inhibit replication of several viruses including influenza virus. A single-nucleotide polymorphism rs12252-C of IFITM3 results in a truncated IFITM3 protein lacking its first 21 amino acids, which is much higher in the Han Chinese population and associated with severe illness in adults infected with pandemic influenza H1N1/09 virus. To investigate if IFITM3 or IFITM3 rs12252-C could affect the antibody response after influenza vaccination, we detected the haemagglutination inhibition (HI) of 171 healthy young adult volunteers (IFITM3 rs12252-C/C, C/T, T/T carriers) and in an IFITM3-deletion mouse model (Ifitm3-/-) after trivalent inactivated vaccine (TIV) immunization. Seroconversion rates for H1N1, H3N2 and B viruses in IFITM3 rs12252-C/C genotype carriers was lower compared with C/T and T/T donors. Significantly lower levels of specific antibodies to H1N1, H3N2 and B viruses and total IgG were observed in Ifitm3-/- mice. Correspondingly, the numbers of splenic germinal centre (GC) B cells, plasma cells, TIV-specific IgG+ antibody secreting cells and T follicular helper cells in Ifitm3-/- mice were lower compared with wild type mice. However, the number of memory B cells was higher in Ifitm3-/- mice at day 7 after booster. The HI level of Ifitm3-/- mice remained lower than WT mice after third vaccination. Moreover, the transcriptional network regulating GC B cell and plasma cell differentiation was abnormal in Ifitm3-/- mice. Our results indicate that IFITM3 deletion attenuated the antibody response. The mechanism of influenza-IFITM3 interactions affecting the antibody response requires further investigation.https://www.tandfonline.com/doi/10.1080/22221751.2020.1756696Interferon-induced transmembrane protein 3IFITM3SNP rs12252influenza virustrivalent inactivated vaccineimmune response |
| spellingShingle | Na Lei Yan Li Qiang Sun Jian Lu Jianfang Zhou Zi Li Liqi Liu Junfeng Guo Kun Qin Haibin Wang Jianhong Zhao Chong Li Lingli Sun Dayan Wang Zhendong Zhao Yuelong Shu IFITM3 affects the level of antibody response after influenza vaccination Emerging Microbes and Infections Interferon-induced transmembrane protein 3 IFITM3 SNP rs12252 influenza virus trivalent inactivated vaccine immune response |
| title | IFITM3 affects the level of antibody response after influenza vaccination |
| title_full | IFITM3 affects the level of antibody response after influenza vaccination |
| title_fullStr | IFITM3 affects the level of antibody response after influenza vaccination |
| title_full_unstemmed | IFITM3 affects the level of antibody response after influenza vaccination |
| title_short | IFITM3 affects the level of antibody response after influenza vaccination |
| title_sort | ifitm3 affects the level of antibody response after influenza vaccination |
| topic | Interferon-induced transmembrane protein 3 IFITM3 SNP rs12252 influenza virus trivalent inactivated vaccine immune response |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1756696 |
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