Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer
A mouse gastric cancer model is an important tool for studying the mechanisms of gastric cancer. To establish subcutaneously implanted tumors, MKN-45 cell suspensions and tumor tissues were implanted into the middle of the right armpit of nude mice. To generate an abdominal metastasis model, MKN-45...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/6856107 |
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| author | Yin Zhu Yi Hu Ming Cheng Chun-Yan Zeng Zhen Yang Xiao-Dong Zhou Jiang Chen Nong-Hua Lu |
| author_facet | Yin Zhu Yi Hu Ming Cheng Chun-Yan Zeng Zhen Yang Xiao-Dong Zhou Jiang Chen Nong-Hua Lu |
| author_sort | Yin Zhu |
| collection | DOAJ |
| description | A mouse gastric cancer model is an important tool for studying the mechanisms of gastric cancer. To establish subcutaneously implanted tumors, MKN-45 cell suspensions and tumor tissues were implanted into the middle of the right armpit of nude mice. To generate an abdominal metastasis model, MKN-45 cell suspensions and tumor tissue homogenates were implanted into the middle of the lower abdomen. We measured the weights of the nude mice and the longest dimension, shortest dimension, thickness, and volume of the tumor. We also analyzed the rate of tumor formation, the time required for tumor formation, and the number and size of abdominal tumors in the mice. The rates of formation of the subcutaneously implanted tumors were 100%, 0%, and 100% in the nude mice inoculated with 2 × 107 cells/mL or 1 × 107 cells/mL of the MKN-45 cell suspension or the tumor tissue homogenate (2 × 107 cells/mL), respectively. The rates of metastatic abdominal tumor formation were 100%, 50%, and 75% in mice inoculated with 5 × 107 cells/mL or 1 × 107 cells/mL of the tumor tissue homogenate or the MKN-45 cell suspension (5 × 107 cells/mL), respectively. We derived tumor tissues and tumor tissue homogenates from nude mice prior to establishing the subcutaneous model of implanted tumors and the abdominal metastasis model of gastric cancer, respectively. |
| format | Article |
| id | doaj-art-0f6a22e508ff4378a46a33dc07102aa4 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-0f6a22e508ff4378a46a33dc07102aa42025-08-20T03:54:11ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/68561076856107Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric CancerYin Zhu0Yi Hu1Ming Cheng2Chun-Yan Zeng3Zhen Yang4Xiao-Dong Zhou5Jiang Chen6Nong-Hua Lu7Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, ChinaA mouse gastric cancer model is an important tool for studying the mechanisms of gastric cancer. To establish subcutaneously implanted tumors, MKN-45 cell suspensions and tumor tissues were implanted into the middle of the right armpit of nude mice. To generate an abdominal metastasis model, MKN-45 cell suspensions and tumor tissue homogenates were implanted into the middle of the lower abdomen. We measured the weights of the nude mice and the longest dimension, shortest dimension, thickness, and volume of the tumor. We also analyzed the rate of tumor formation, the time required for tumor formation, and the number and size of abdominal tumors in the mice. The rates of formation of the subcutaneously implanted tumors were 100%, 0%, and 100% in the nude mice inoculated with 2 × 107 cells/mL or 1 × 107 cells/mL of the MKN-45 cell suspension or the tumor tissue homogenate (2 × 107 cells/mL), respectively. The rates of metastatic abdominal tumor formation were 100%, 50%, and 75% in mice inoculated with 5 × 107 cells/mL or 1 × 107 cells/mL of the tumor tissue homogenate or the MKN-45 cell suspension (5 × 107 cells/mL), respectively. We derived tumor tissues and tumor tissue homogenates from nude mice prior to establishing the subcutaneous model of implanted tumors and the abdominal metastasis model of gastric cancer, respectively.http://dx.doi.org/10.1155/2017/6856107 |
| spellingShingle | Yin Zhu Yi Hu Ming Cheng Chun-Yan Zeng Zhen Yang Xiao-Dong Zhou Jiang Chen Nong-Hua Lu Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer Gastroenterology Research and Practice |
| title | Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer |
| title_full | Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer |
| title_fullStr | Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer |
| title_full_unstemmed | Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer |
| title_short | Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer |
| title_sort | establishment and characterization of a nude mouse model of subcutaneously implanted tumors and abdominal metastasis in gastric cancer |
| url | http://dx.doi.org/10.1155/2017/6856107 |
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