The BnaBPs gene regulates flowering time and leaf angle in Brassica napus
Abstract The flowering time and plant architecture of Brassica napus were significantly associated with yield. In this study, we found that the BREVIPEDICELLUS/KNAT1(BP) gene regulated the flowering time and plant architecture of B. napus. However, the precise regulatory mechanism remains unclear. W...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-10-01
|
| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.70018 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832541386845454336 |
|---|---|
| author | Jiang Yu Yi‐Xuan Xue Rehman Sarwar Shi‐Hao Wei Rui Geng Yan‐Feng Zhang Jian‐Xin Mu Xiao‐Li Tan |
| author_facet | Jiang Yu Yi‐Xuan Xue Rehman Sarwar Shi‐Hao Wei Rui Geng Yan‐Feng Zhang Jian‐Xin Mu Xiao‐Li Tan |
| author_sort | Jiang Yu |
| collection | DOAJ |
| description | Abstract The flowering time and plant architecture of Brassica napus were significantly associated with yield. In this study, we found that the BREVIPEDICELLUS/KNAT1(BP) gene regulated the flowering time and plant architecture of B. napus. However, the precise regulatory mechanism remains unclear. We cloned two homologous BP genes, BnaBPA03 and BnaBPC03, from B. napus Xiaoyun. The protein sequence analysis showed two proteins containing conserved domains KNOX I, KNOX II, ELK, and HOX of the KONX protein family. The CRISPR/Cas9 knockout lines exhibited early budding and flowering time, coupled with floral organ abscission earlier and a larger leaf angle. On the contrary, overexpression plants displayed a phenotype that was the inverse of these characteristics. Furthermore, we observed upregulation of gibberellin and ethylene biosynthesis genes, as well as floral integrator genes in knocked‐out plants. The results revealed that BnaBPs play a role in flowering time, floral organ abscission, and leaf angle as well as germination processes mediated. Additionally, BnaBPs exerted an impact on the biosynthesis pathways of ethylene and GA. |
| format | Article |
| id | doaj-art-0f641776a50b403480600b7e18ddd548 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-0f641776a50b403480600b7e18ddd5482025-02-04T08:31:57ZengWileyPlant Direct2475-44552024-10-01810n/an/a10.1002/pld3.70018The BnaBPs gene regulates flowering time and leaf angle in Brassica napusJiang Yu0Yi‐Xuan Xue1Rehman Sarwar2Shi‐Hao Wei3Rui Geng4Yan‐Feng Zhang5Jian‐Xin Mu6Xiao‐Li Tan7School of Life Sciences Jiangsu University Zhenjiang Jiangsu ChinaSchool of Life Sciences Jiangsu University Zhenjiang Jiangsu ChinaSchool of Life Sciences Jiangsu University Zhenjiang Jiangsu ChinaHybrid Rape Research Center Shaanxi Prov Yangling Shanxi ChinaSchool of Life Sciences Jiangsu University Zhenjiang Jiangsu ChinaHybrid Rape Research Center Shaanxi Prov Yangling Shanxi ChinaHybrid Rape Research Center Shaanxi Prov Yangling Shanxi ChinaSchool of Life Sciences Jiangsu University Zhenjiang Jiangsu ChinaAbstract The flowering time and plant architecture of Brassica napus were significantly associated with yield. In this study, we found that the BREVIPEDICELLUS/KNAT1(BP) gene regulated the flowering time and plant architecture of B. napus. However, the precise regulatory mechanism remains unclear. We cloned two homologous BP genes, BnaBPA03 and BnaBPC03, from B. napus Xiaoyun. The protein sequence analysis showed two proteins containing conserved domains KNOX I, KNOX II, ELK, and HOX of the KONX protein family. The CRISPR/Cas9 knockout lines exhibited early budding and flowering time, coupled with floral organ abscission earlier and a larger leaf angle. On the contrary, overexpression plants displayed a phenotype that was the inverse of these characteristics. Furthermore, we observed upregulation of gibberellin and ethylene biosynthesis genes, as well as floral integrator genes in knocked‐out plants. The results revealed that BnaBPs play a role in flowering time, floral organ abscission, and leaf angle as well as germination processes mediated. Additionally, BnaBPs exerted an impact on the biosynthesis pathways of ethylene and GA.https://doi.org/10.1002/pld3.70018BnaBPBrassica napusflowering timeKNOX protein familyplant architecture |
| spellingShingle | Jiang Yu Yi‐Xuan Xue Rehman Sarwar Shi‐Hao Wei Rui Geng Yan‐Feng Zhang Jian‐Xin Mu Xiao‐Li Tan The BnaBPs gene regulates flowering time and leaf angle in Brassica napus Plant Direct BnaBP Brassica napus flowering time KNOX protein family plant architecture |
| title | The BnaBPs gene regulates flowering time and leaf angle in Brassica napus |
| title_full | The BnaBPs gene regulates flowering time and leaf angle in Brassica napus |
| title_fullStr | The BnaBPs gene regulates flowering time and leaf angle in Brassica napus |
| title_full_unstemmed | The BnaBPs gene regulates flowering time and leaf angle in Brassica napus |
| title_short | The BnaBPs gene regulates flowering time and leaf angle in Brassica napus |
| title_sort | bnabps gene regulates flowering time and leaf angle in brassica napus |
| topic | BnaBP Brassica napus flowering time KNOX protein family plant architecture |
| url | https://doi.org/10.1002/pld3.70018 |
| work_keys_str_mv | AT jiangyu thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT yixuanxue thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT rehmansarwar thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT shihaowei thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT ruigeng thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT yanfengzhang thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT jianxinmu thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT xiaolitan thebnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT jiangyu bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT yixuanxue bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT rehmansarwar bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT shihaowei bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT ruigeng bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT yanfengzhang bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT jianxinmu bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus AT xiaolitan bnabpsgeneregulatesfloweringtimeandleafangleinbrassicanapus |