Prevalence of human papillomavirus genotype among Moroccan women during a local screening program
Introduction: Many studies have indicated a causal relationship between genital human papillomavirus (HPV) infections and cervical cancer. This study aimed to determine the prevalence and genotypes of six high-risk oncogenic human papillomaviruses in cervical lesions from Moroccan women with normal...
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The Journal of Infection in Developing Countries
2010-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/781 |
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| author | Zaitouna Alhamany Mohammed El Mzibri Aicha Kharbach Abderrahman Malihy Redouane Abouqal Hassan Jaddi Ali Benomar Mohammed Attaleb Najat Lamalmi Nadia Cherradi |
| author_facet | Zaitouna Alhamany Mohammed El Mzibri Aicha Kharbach Abderrahman Malihy Redouane Abouqal Hassan Jaddi Ali Benomar Mohammed Attaleb Najat Lamalmi Nadia Cherradi |
| author_sort | Zaitouna Alhamany |
| collection | DOAJ |
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Introduction: Many studies have indicated a causal relationship between genital human papillomavirus (HPV) infections and cervical cancer. This study aimed to determine the prevalence and genotypes of six high-risk oncogenic human papillomaviruses in cervical lesions from Moroccan women with normal and abnormal cytology.
Methodology: The study included 938 women from the Children's and Mothers' Pathology Department of Ibn Sina Hospital, Rabat. Cytopathology examination was done by routine PAP smear testing. HPV DNA testing was conducted using DNA amplification by Polymerase Chain Reaction with subsequent typing by hybridization with specific probes for HPV types 16, 18, 31, 33, 35 and 45.
Results: Cytopathology testing showed that only 16.3 % had an abnormal cytology, with a predominance of atypical squamous cell of undetermined significance (ASCUS) cases. The overall HPV prevalence was 15.7%. According to the cytology results, HPV infection was detected in 15.8% of normal and 14.38% of abnormal cases. Specific HPV genotyping showed a predominance of HPV 16 and 18. Double infection (HPV 16 + 18) was found in two cases whereas multiple infections (HPV 16+18+31) were detected in only one case.
Evaluation of the relationship between HPV status and some environmental risk factors, including individual, socio-economic, and hygiene status, showed a significant association between HPV infection and oral contraceptive use.
Conclusion: Based on these data, a combination of cytology and HPV DNA testing allows for identification of patients with a high risk of developing high-grade cervical lesions and improves cervical cancer prevention.
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| format | Article |
| id | doaj-art-0f635992c38245a0a24eb97a712aa4bb |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2010-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-0f635992c38245a0a24eb97a712aa4bb2025-08-20T02:14:22ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802010-09-0141110.3855/jidc.781Prevalence of human papillomavirus genotype among Moroccan women during a local screening programZaitouna Alhamany0Mohammed El Mzibri1Aicha Kharbach2Abderrahman Malihy3Redouane Abouqal4Hassan Jaddi5Ali Benomar6Mohammed Attaleb7Najat Lamalmi8Nadia Cherradi9Maternité Souissi, Centre Hospitalier Universitaire Ibn Sina. Rabat, MoroccoUnité de Biologie et Recherches Médicales, Centre National de l’Energie, des Sciences et Techniques Nucléaires, Rabat, MoroccoDépartement de Gynécologie Obstétrique. Maternité Souissi, Centre Hospitalier Universitaire Ibn Sina, RabatDépartement d’Anatomie pathologique. Maternité Souissi, Centre Hospitalier Universitaire Ibn Sina. RabatFaculté de Médecine et de Pharmacie. Université Mohamed V Souissi. RabatUnité de Biologie et Recherches Médicales, Centre National de l’Energie, des Sciences et Techniques Nucléaires, Rabat, MoroccoFaculté de Médecine et de Pharmacie. Université Mohamed V Souissi. RabatUnité de Biologie et Recherches Médicales, Centre National de l’Energie, des Sciences et Techniques Nucléaires, Rabat, MoroccoMaternité Souissi, Centre Hospitalier Universitaire Ibn Sina. RabatMaternité Souissi, Centre Hospitalier Universitaire Ibn Sina. Rabat Introduction: Many studies have indicated a causal relationship between genital human papillomavirus (HPV) infections and cervical cancer. This study aimed to determine the prevalence and genotypes of six high-risk oncogenic human papillomaviruses in cervical lesions from Moroccan women with normal and abnormal cytology. Methodology: The study included 938 women from the Children's and Mothers' Pathology Department of Ibn Sina Hospital, Rabat. Cytopathology examination was done by routine PAP smear testing. HPV DNA testing was conducted using DNA amplification by Polymerase Chain Reaction with subsequent typing by hybridization with specific probes for HPV types 16, 18, 31, 33, 35 and 45. Results: Cytopathology testing showed that only 16.3 % had an abnormal cytology, with a predominance of atypical squamous cell of undetermined significance (ASCUS) cases. The overall HPV prevalence was 15.7%. According to the cytology results, HPV infection was detected in 15.8% of normal and 14.38% of abnormal cases. Specific HPV genotyping showed a predominance of HPV 16 and 18. Double infection (HPV 16 + 18) was found in two cases whereas multiple infections (HPV 16+18+31) were detected in only one case. Evaluation of the relationship between HPV status and some environmental risk factors, including individual, socio-economic, and hygiene status, showed a significant association between HPV infection and oral contraceptive use. Conclusion: Based on these data, a combination of cytology and HPV DNA testing allows for identification of patients with a high risk of developing high-grade cervical lesions and improves cervical cancer prevention. https://jidc.org/index.php/journal/article/view/781HPVcervical cancerPCRMolecular hybridizationMorocco |
| spellingShingle | Zaitouna Alhamany Mohammed El Mzibri Aicha Kharbach Abderrahman Malihy Redouane Abouqal Hassan Jaddi Ali Benomar Mohammed Attaleb Najat Lamalmi Nadia Cherradi Prevalence of human papillomavirus genotype among Moroccan women during a local screening program Journal of Infection in Developing Countries HPV cervical cancer PCR Molecular hybridization Morocco |
| title | Prevalence of human papillomavirus genotype among Moroccan women during a local screening program |
| title_full | Prevalence of human papillomavirus genotype among Moroccan women during a local screening program |
| title_fullStr | Prevalence of human papillomavirus genotype among Moroccan women during a local screening program |
| title_full_unstemmed | Prevalence of human papillomavirus genotype among Moroccan women during a local screening program |
| title_short | Prevalence of human papillomavirus genotype among Moroccan women during a local screening program |
| title_sort | prevalence of human papillomavirus genotype among moroccan women during a local screening program |
| topic | HPV cervical cancer PCR Molecular hybridization Morocco |
| url | https://jidc.org/index.php/journal/article/view/781 |
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